Dhrs7 (NM_001271394) Rat Tagged ORF Clone

CAT#: RR216403

RGD1565002 (myc-DDK-tagged) - Rat similar to Dehydrogenase/reductase SDR family member 7 precursor (Retinal short-chain dehydrogenase/reductase 4) (RGD1565002)

Product Images

Circular map for RR216403

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dhrs7
• Synonyms: RGD1565002
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216403 representing NM_001271394
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGGGAGCTGCTGCTCTGGCTGCTGGCGCTGTGCGCAGTGATCCTGCCCCTGGTGCAGTTCCTAC
  GCTTCCTGCGGGCAGATGCCGACCTAACCTTGTTGTGGGCCGAGTGGCAAGGGCGACGCCCAGAATGGGA
  GCTGACCGACATGGTGGTGTGGATTACTGGAGCATCAAGCGGCATTGGTGAGGAGCTGGCTTTCCAGTTA
  TCTAAACTGGGGGTCTGTCTGGTGCTGTCAGCCCGAAGGGGGCAGGAGCTGGAGCGGGTGAAGAGAAGAT
  GCCTGGAGAATGGCAATTTAAAAGAAAAAGATATACTGGTTCTGCCCCTTGACTTGACTGACACAAGTTC
  CCATGAGGCAGCGACCAAAGCCGTTCTGCAGGAATTTGGTAAAATTGACATTTTGGTCAACAACGGTGGA
  AGATCCCAGCGTTCCTTAGTTCTGGAAACCAACTTGGAAGTCTTCAAGGAGCTGATGAATCTGAACTACT
  TAGGGACGGTGTCCTTGACCAAGTGTGTTCTGCCTCACATGGTAGAGAGGAAACAAGGGAAGATTGTTAC
  TGTGAATAGCCTTGCGGGAATTGCATCGGTGTCCCTTTCCAGTGGCTACTGTGCCAGCAAGCATGCTCTT
  CGGGGATTTTTCAATGCCCTCCATAGTGAGCTTGGCAAATATCCAGGCATAACACTTTGCAACGTTTACC
  CAGGGCCTGTGCAGTCTAATGTTGTGAAGAACGCCCTAACTGAAGAATTAACGAAGCCCATGAGAGAGAA
  TATAGACCAGAGTTACAAGATGCCGACAAGCCGCTGTGTGCGGCTGATGCTCATCTCCATGGCCAACGAT
  CTGAAGGAAGTTTGGATCTCTGACCAACCCATATTGTTGGGTGCATACATTTGGCAGTATATGCCAACCT
  GGGCCTTATGGCTAACCTGCAAGTTGGGGGAGAAAAGAATCCAAAACTTTAGGAATAATTTGGATCCAGA
  CTCGCAATATACATTGTGGAAGCCAAAGAGGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216403 representing NM_001271394
  Red=Cloning site Green=Tags(s)
  
  MSWELLLWLLALCAVILPLVQFLRFLRADADLTLLWAEWQGRRPEWELTDMVVWITGASSGIGEELAFQL
  SKLGVCLVLSARRGQELERVKRRCLENGNLKEKDILVLPLDLTDTSSHEAATKAVLQEFGKIDILVNNGG
  RSQRSLVLETNLEVFKELMNLNYLGTVSLTKCVLPHMVERKQGKIVTVNSLAGIASVSLSSGYCASKHAL
  RGFFNALHSELGKYPGITLCNVYPGPVQSNVVKNALTEELTKPMRENIDQSYKMPTSRCVRLMLISMAND
  LKEVWISDQPILLGAYIWQYMPTWALWLTCKLGEKRIQNFRNNLDPDSQYTLWKPKRD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271394
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271394.1, NP_001258323.1
• RefSeq Size: 1285 bp
• RefSeq ORF: 1017 bp
• Locus ID: 299135
• Cytogenetics: 6q24
• MW: 38.3 kDa