Rnaseh1 (NM_001286938) Rat Tagged ORF Clone

CAT#: RR216176

Rnaseh1 (myc-DDK-tagged) - Rat ribonuclease H1 (Rnaseh1), transcript variant 1

Product Images

Circular map for RR216176

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnaseh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216176 representing NM_001286938
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTATGCGGTGAGGAGAGGCCGCAGGACCGGGGTCTTCCTGAGTTGGAGTGAGTGCAAAGCACAGG
  TGGACCGGTTCCCTGCTGCCAGGTTTAAGAAATTTGCCACAGAAGATGAGGCCTGGGCCTTTGTCAGGAG
  CTCTTCCAGTCCGGACGGTTCAAAAGGGCAGGAGAGTGCGCATGTGCAAAAGTTACAGGTGAAGACCAGC
  AAGCGGCCTCGGGAGCCTCTGGGTGAAGAGGAGGAACCTCCAGAGCCAGGGGCAAAGCACACAAGACAGG
  ACACGGAGCCAGCTGCTCTAGTGAGCAAGGATGCATTTTCTTATATGGGAGAGTCAGTCGTTGTCTACAC
  GGATGGCTGTTGCTCCAGTAATGGGCGGAAGCGGGCACGAGCAGGAATCGGCGTTTACTGGGGGCCAGGC
  CACCCCTTAAATGTAGGCATAAGGCTTCCTGGGCGACAGACAAACCAGAGGGCTGAGATCCATGCAGCCT
  GTAAAGCCATCACGCAAGCCAAGGCTCAGAACATCAGCAAGCTGGTCCTGTACACAGACAGCATGTTCAC
  CATCAACGGGATAACTAACTGGGTTCAGGGCTGGAAGAAGAATGGCTGGAGAACGAGTACAGGGAAAGAC
  GTGATCAACAAGGAGGACTTCATGGAGCTGGATGAGCTCACCCAGGGCATGGACATCCAGTGGATGCACA
  TTCCTGGCCACTCAGGATTTGTGGGCAACGAAGAAGCTGACAGACTGGCAAGGGAAGGAGCGAAGCAATC
  TGAGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216176 representing NM_001286938
  Red=Cloning site Green=Tags(s)
  
  MFYAVRRGRRTGVFLSWSECKAQVDRFPAARFKKFATEDEAWAFVRSSSSPDGSKGQESAHVQKLQVKTS
  KRPREPLGEEEEPPEPGAKHTRQDTEPAALVSKDAFSYMGESVVVYTDGCCSSNGRKRARAGIGVYWGPG
  HPLNVGIRLPGRQTNQRAEIHAACKAITQAKAQNISKLVLYTDSMFTINGITNWVQGWKKNGWRTSTGKD
  VINKEDFMELDELTQGMDIQWMHIPGHSGFVGNEEADRLAREGAKQSEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286938
• ORF Size: 777 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286938.1, NP_001273867.1
• RefSeq Size: 1400 bp
• RefSeq ORF: 780 bp
• Locus ID: 298933
• UniProt ID: Q5BK46
• Cytogenetics: 6q16
• MW: 28.8 kDa
• Gene Summary: This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which encodes 7aa in rat. [provided by RefSeq, Nov 2013]