Ass1 (NM_013157) Rat Tagged ORF Clone

CAT#: RR214954

Ass1 (Myc-DDK-tagged ORF) - Rat argininosuccinate synthetase 1 (Ass1), (10 ug)

Product Images

Circular map for RR214954

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ass1
• Synonyms: Ass; ASSA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214954 representing NM_013157
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGCAAGGGCTCTGTGGTTCTGGCCTACAGTGGTGGTCTGGACACCTCCTGCATCCTCGTGTGGC
  TGAAGGAACAAGGCTATGATGTCATCGCCTACCTGGCCAACATTGGCCAGAAGGAAGACTTTGAGGAAGC
  CAGGAAGAAGGCACTGAAGCTTGGGGCCAAAAAGGTGTTCATTGAGGATGTAAGCAAGGAGTTTGTGGAA
  GAGTTCATCTGGCCTGCTGTCCAGTCCAGTGCACTCTATGAGGACCGCTATCTCCTAGGCACCTCTCTCG
  CCAGGCCTTGCATAGCTCGCAAACAAGTGGAAATTGCCCAGCGCGAAGGGGCCAAGTATGTGTCTCACGG
  CGCCACGGGGAAGGGCAATGACCAGGTCCGCTTTGAGCTCACCTGCTACTCGTTAGCACCCCAGATTAAG
  GTCATCGCCCCCTGGAGGATGCCCGAGTTTTACAACCGGTTCAAGGGCCGAAATGATTTGATGGAATACG
  CAAAGCAACATGGAATCCCCATCCCTGTCACCCCCAAGAGCCCCTGGAGCATGGATGAGAACCTTATGCA
  CATCAGCTACGAGGCTGGAATCCTGGAAAACCCCAAGAACCAAGCACCTCCAGGTCTCTACACAAAAACT
  CAGGACCCTGCCAAAGCACCCAACACCCCAGATGTCCTTGAGATAGAATTCAAAAAAGGGGTCCCTGTGA
  AGGTGACCAACGTCAAAGATGGCACTACCCACAGCACATCCTTGGACCTCTTCATGTACCTGAATGAAGT
  TGCGGGCAAGCATGGAGTAGGGCGCATTGACATCGTGGAGAACCGCTTCATTGGAATGAAGTCCCGGGGT
  ATCTACGAGACCCCAGCAGGGACCATCCTTTACCACGCTCATTTAGACATAGAGGCCTTCACCATGGATC
  GGGAAGTACGCAAAATCAAGCAGGGCCTGGGCCTCAAATTCGCAGAGCTCGTATACACCGGTTTCTGGCA
  CAGCCCTGAATGTGAATTTGTTCGCCACTGCATCGACAAGTCCCAGGAACGGGTGGAAGGAAAGGTGCAG
  GTATCTGTCTTCAAGGGCCAGGTGTACATCCTTGGCCGGGAGTCTCCACTTTCACTATACAATGAAGAGC
  TGGTGAGCATGAACGTACAGGGTGACTATGAACCCATTGATGCCACCGGCTTCATCAATATCAACTCGCT
  CAGGCTGAAGGAGTACCATCGCCTTCAGAGCAAGGTCACCGCCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214954 representing NM_013157
  Red=Cloning site Green=Tags(s)
  
  MSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSKEFVE
  EFIWPAVQSSALYEDRYLLGTSLARPCIARKQVEIAQREGAKYVSHGATGKGNDQVRFELTCYSLAPQIK
  VIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKSPWSMDENLMHISYEAGILENPKNQAPPGLYTKT
  QDPAKAPNTPDVLEIEFKKGVPVKVTNVKDGTTHSTSLDLFMYLNEVAGKHGVGRIDIVENRFIGMKSRG
  IYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIDKSQERVEGKVQ
  VSVFKGQVYILGRESPLSLYNEELVSMNVQGDYEPIDATGFININSLRLKEYHRLQSKVTAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013157
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013157.3, NP_037289.1
• RefSeq Size: 1495 bp
• RefSeq ORF: 1239 bp
• Locus ID: 25698
• UniProt ID: P09034
• Cytogenetics: 3p12
• MW: 46.5 kDa
• Gene Summary: catalyzes the conversion of ATP, L-citrulline, and L-aspartate to AMP, diphosphate, and L-argininosuccinate in arginine biosynthesis; may regulate nitric oxide production [RGD, Feb 2006]