Rasgrp2 (NM_001082977) Rat Tagged ORF Clone

CAT#: RR214269

Rasgrp2 (Myc-DDK-tagged ORF) - Rat RAS guanyl releasing protein 2 (calcium and DAG-regulated) (Rasgrp2), (10 ug)

Product Images

Circular map for RR214269

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rasgrp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214269 representing NM_001082977
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCACTCTGGACCTGGACAAGGGTTGCACCGTGGAGGAGCTGCTCCGTGGCTGTATCGAAGCCT
  TTGATGACTCTGGAAAGGTGCGAGATCCACAGCTAGTGCGCATGTTTCTCATGATGCACCCCTGGTACAT
  ACCTTCCTCTCAGCTGGCTTCGAAACTGCTCCACTTCTATCAGCAATCCCGGAAGGACAACTCCAATTCC
  CTACAGGTGAAAACCTGTCACCTGGTCAGGTACTGGGTCTCAGCCTTCCCAGCAGAGTTCGACTTGAACC
  CAGAGCTGGCTGAACAGATCAAGGAGCTGAAGGCTCTGTTAGACCAAGAAGGAAACCGCAGGCACAGCAG
  CCTCATCGACATCGAGAGTGTCCCCACCTACAAGTGGAAGCGGCAGGTGACCCAGCGGAACCCTGTGGAA
  CAGAAAAAGCGCAAGATGTCCCTGTTGTTTGATCACTTGGAGCCTATGGAACTGGCAGAACATCTCACCT
  ACTTGGAGTATCGGTCCTTCTGCAAGATCCTGTTCCAGGACTATCACAGCTTTGTGACTCATGGCTGCAC
  TGTAGACAATCCGGTCCTGGAGCGATTCATCTCCCTCTTCAACAGTGTCTCTCAGTGGGTCCAACTCATG
  ATCCTCAGCAAGCCCACAGCCACGCAGCGGGCGCTGGTCATCACACATTTCGTGCATGTGGCAGAGAAGC
  TGCTGCAGCTGCAGAACTTCAACACGTTGATGGCCGTCGTGGGAGGCCTGAGCCACAGCTCCATCTCACG
  CCTCAAGGAGACCCACAGCCATGTCAGCCCTGACACCATCAAGCTCTGGGAAGGTCTGACAGAACTAGTG
  ACAGCTACTGGCAACTACAGCAACTACCGGCGAAGGCTGGCGGCCTGCGTGGGCTTCCGCTTTCCTATCC
  TGGGTGTGCACCTCAAGGATCTAGTGGCTCTGCAGCTGGCTCTGCCTGACTGGCTGGACCCAGGTCGGAC
  CCGGCTCAATGGAGCCAAGATGAGGCAGCTTTTCAGCATTCTGGAGGAGTTGGCTATGGTGACCAGTCTT
  CGACCACCAGTGCAAGCCAACCCCGACCTGCTGAGTCTGCTCACGGTGTCCCTGGACCAGTATCAGACGG
  AGGATGAGCTGTATCAGCTCTCTCTGCAGCGAGAGCCACGTTCCAAGTCATCGCCCACCAGCCCCACCAG
  CTGCACCCCGCCTCCCCGGCCGCCTGTGCTGGAAGAGTGGACCTCAGTTGCCAAGCCTAAGCTGGACCAA
  GCCTTGGTGGCCGAGCACATTGAGAAGATGGTGGAGTCTGTGTTCCGGAACTTTGACGTTGATGGGGACG
  GTCACATCTCCCAGGAGGAGTTCCAGATCATCCGGGGCAACTTCCCTTATCTCAGCGCCTTTGGGGACTT
  GGACCAGAACCAGGATGGCTGCATCAGCCGCGAGGAGATGATCTCCTACTTCCTGCGCTCCAGCTCGGTG
  CTGGGAGGCCGCATGGGCTTCGTACACAACCTCCAGGAGAGTAACTCGCTAAGGCCGGTCGCCTGCCGAC
  ACTGCAAAGCTCTGATCCTGGGCATCTACAAGCAGGGCCTCAAATGTAGAGCTTGTGGTGTGAACTGCCA
  CAAGCAGTGCAAAGACCGACTGTCAGTGGAATGTCGCCGCCGCGCCCAGAGTGTGAGCCTGGAGGGCTCT
  GCACCCTCTCCCTCACCCACACATACCCACCATCGGGCCTTCAGCTTCTCCCTGCCTCGCCCAGGCAGGC
  GCAGCTCTCGGCCTCCAGAGATCCGTGAAGAGGAGGTGCAGACTGTGGAAGATGGTGTGTTCGACATCCA
  CTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214269 representing NM_001082977
  Red=Cloning site Green=Tags(s)
  
  MASTLDLDKGCTVEELLRGCIEAFDDSGKVRDPQLVRMFLMMHPWYIPSSQLASKLLHFYQQSRKDNSNS
  LQVKTCHLVRYWVSAFPAEFDLNPELAEQIKELKALLDQEGNRRHSSLIDIESVPTYKWKRQVTQRNPVE
  QKKRKMSLLFDHLEPMELAEHLTYLEYRSFCKILFQDYHSFVTHGCTVDNPVLERFISLFNSVSQWVQLM
  ILSKPTATQRALVITHFVHVAEKLLQLQNFNTLMAVVGGLSHSSISRLKETHSHVSPDTIKLWEGLTELV
  TATGNYSNYRRRLAACVGFRFPILGVHLKDLVALQLALPDWLDPGRTRLNGAKMRQLFSILEELAMVTSL
  RPPVQANPDLLSLLTVSLDQYQTEDELYQLSLQREPRSKSSPTSPTSCTPPPRPPVLEEWTSVAKPKLDQ
  ALVAEHIEKMVESVFRNFDVDGDGHISQEEFQIIRGNFPYLSAFGDLDQNQDGCISREEMISYFLRSSSV
  LGGRMGFVHNLQESNSLRPVACRHCKALILGIYKQGLKCRACGVNCHKQCKDRLSVECRRRAQSVSLEGS
  APSPSPTHTHHRAFSFSLPRPGRRSSRPPEIREEEVQTVEDGVFDIHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001082977
• ORF Size: 1824 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001082977.1, NP_001076446.1
• RefSeq Size: 2228 bp
• RefSeq ORF: 1827 bp
• Locus ID: 361714
• UniProt ID: P0C643
• Cytogenetics: 1q43
• MW: 69.3 kDa
• Gene Summary: Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.[UniProtKB/Swiss-Prot Function]