Kcnk10 (NM_023096) Rat Tagged ORF Clone

CAT#: RR212265

Kcnk10 (Myc-DDK-tagged ORF) - Rat potassium channel, subfamily K, member 10 (Kcnk10), (10 ug)

Product Images

Circular map for RR212265

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnk10
• Synonyms: TREK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212265 representing NM_023096
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATTTCCAATCGAGACGCCAAGAAAGCAGGTGAACTGGGATCCTAAAGTGGCCGTGCCTGCAGCAG
  CCCCACCAGTGTGCCAGCCCAAGAGTGCCACTAACGGGCACCATCCTGTTCCTCGACTCTCCATTTCCTC
  CAGAGCCACGGTGGTAGCCAGGATGGAAGGCGCCTCCCAGGGAGGCCTGCAAACAGTTATGAAATGGAAA
  ACAGTGGTTGCCATCTTTGTGGTGGTCGTGGTCTACCTCGTCACCGGTGGCCTTGTCTTCCGGGCATTGG
  AACAGCCCTTTGAGAGCAGTCAGAAGAACACAATTGCCTTGGAGAAGGCAGAATTCTTGAGAGATCATAT
  CTGTGTGAGCCCCCAGGAACTGGAGACACTGATCCAGCACGCACTTGATGCTGATAATGCAGGGGTCAGC
  CCGGTAGGAAACTCCTCCAACAGCAGCAGTCACTGGGACCTCGGAAGTGCCTTCTTCTTTGCTGGGACGG
  TCATCACGACCATAGGGTATGGGAATATTGCTCCGAGTACTGAAGGAGGCAAAATCTTTTGTATTTTATA
  CGCCATCTTTGGGATCCCGCTCTTCGGTTTCTTATTGGCTGGAATTGGAGACCAACTTGGAACCATCTTT
  GGCAAAAGCATTGCAAGAGTGGAAAAGGTTTTCCGAAAAAAGCAAGTGAGTCAGACCAAGATCCGGGTCA
  TCTCAACTATTCTTTTCATCTTGGCTGGCTGCATCGTGTTTGTGACGATCCCTGCTGTCATCTTTAAATA
  CATAGAGGGCTGGACCGCCTTGGAGTCCATCTACTTTGTGGTAGTCACTCTGACCACAGTGGGCTTTGGT
  GATTTTGTGGCAGGGGGAAATGCTGGCATCAATTACCGAGAATGGTACAAGCCGCTGGTGTGGTTTTGGA
  TCCTTGTTGGCCTTGCCTACTTTGCAGCTGTCCTCAGTATGATTGGAGACTGGCTGCGAGTTTTATCCAA
  AAAGACAAAAGAAGAGGTGGGTGAGATCAAAGCCCATGCAGCTGAATGGAAGGCTAATGTCACTGCTGAG
  TTCCGGGAGACGAGACGACGGCTCAGCGTTGAGATCCATGACAAGCTGCAACGGGCAGCCACCATCCGAA
  GTATGGAGCGCCGGAGACTGGGATTGGACCAGAGGGCCCACTCACTGGACATGCTTTCCCCCGAGAAGCG
  CTCTGTCTTTGCAGCCCTGGACACAGGCCGTTTCAAGGCCTCATCCCAGGAGAGTATCAACAACAGACCC
  AATAACCTACGCCTTAAGGGGCCAGAACAGCTCAACAAACACGGGCAGGGCGCTTCTGAGGACAACATCA
  TCAACAAGTTTGGGTCCACCTCCAAACTCACAAAGAGGAAAAACAAAGATCTCAAAAAGACCTTACCTGA
  GGATGTTCAGAAAATCTACAAAACCTTCCGGAATTACTCTCTGGATGAAGAGAAGAAAGAGGACGAGACA
  GAGAAGATGTGTAACTCAGACAACTCCAGCACAGCCATGCTGACAGAGTGCATACAGCAGCAAGCTGAGA
  TGGAGAATGGAATGGTACCCATGGACACCAAAGACCAGGGGCTGGAGAACAATTCTTTACTTGAAGACAG
  AAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212265 representing NM_023096
  Red=Cloning site Green=Tags(s)
  
  MKFPIETPRKQVNWDPKVAVPAAAPPVCQPKSATNGHHPVPRLSISSRATVVARMEGASQGGLQTVMKWK
  TVVAIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHICVSPQELETLIQHALDADNAGVS
  PVGNSSNSSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIF
  GKSIARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFG
  DFVAGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEIKAHAAEWKANVTAE
  FRETRRRLSVEIHDKLQRAATIRSMERRRLGLDQRAHSLDMLSPEKRSVFAALDTGRFKASSQESINNRP
  NNLRLKGPEQLNKHGQGASEDNIINKFGSTSKLTKRKNKDLKKTLPEDVQKIYKTFRNYSLDEEKKEDET
  EKMCNSDNSSTAMLTECIQQQAEMENGMVPMDTKDQGLENNSLLEDRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023096
• ORF Size: 1614 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023096.2, NP_075584.1
• RefSeq Size: 3056 bp
• RefSeq ORF: 1617 bp
• Locus ID: 65272
• UniProt ID: Q9JIS4
• Cytogenetics: 6q32
• MW: 59.8 kDa
• Gene Summary: tandem-pore K(+) channel that is a mechanosensitive and fatty acid-stimulated K(+) channel [RGD, Feb 2006]