Egln2 (NM_001004083) Rat Tagged ORF Clone

CAT#: RR212035

Egln2 (Myc-DDK-tagged ORF) - Rat EGL nine homolog 2 (C. elegans) (Egln2), (10 ug)

Product Images

Circular map for RR212035

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Egln2
• Synonyms: HIF-PH1; HPH-1; HPH-3; PHD-1; PHD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212035 representing NM_001004083
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCCCGTGCCAGCCGCAGGCCCTGAATCAAGCTCTCCCTCAGTTGCCAGGGTCTGTGTCAGAGT
  CCTTGGAGCCTAGCCGAGCCAGGATGGGGGTGGAGAGTTACCTGCCCTGTCCTCTGCTCCCCTCCTACCA
  CCGTTCAGGAGCATCTGGGGAGGCCTCGGCTGGCAACGGGACCCCCAGAACCACAGCCACTGCTACTACT
  ACCACTGCCAGTCCCCTGCGGGAGGGCTTTGGTGGGCAGGATGGTGGTGAGCTGTGGCCACTGCAGAGTG
  AAGGTGCTGCTGCGTTGGTTACCAAGGAGTGCCAGCGACTGGCGGCCCAGGGTGCCCGACCTGAGGCCCC
  CAAACGGAAGTGGGCCAAGGATGGTGGGGATGCCCCTTCACCCAGCAAGCGACCGTGGGCCAGGCAAGAG
  AACCAGGAGGCCAAGGGGGAAAGTGGTGTGGGCTGTGACAGCGGTGGCGGCAGCAGCAACAGCACTACCC
  ATAGCAGTGGCGAGGCAAGTTCTAGGCTGAGGGAGGAAGCCCAGCCCTCTGCACCTGAGCGCCTGGCCTT
  GGACTATATTGTGCCTTGCATGCGGTACTATGGTATCTGCGTCAAGGACAACTTCTTGGGGGCAGTACTG
  GGTGGCCGTGTGCTGGCTGAGGTGGAAGCCCTGAAGTGGGGCGGGCGTCTACGTGATGGGCAACTAGTGA
  GCCAGCGGGCGATCCCACCGCGCAGCATTCGTGGGGACCAGATTGCCTGGGTAGAAGGCCACGAGCCAGG
  CTGCCGGAGCATTGGTGCCCTCATGGCTCATGTGGACGCAGTAATCCGCCACTGTGCAGGGCGGCTGGGC
  AACTACGTCATCAATGGGCGCACCAAGGCCATGGTGGCGTGTTACCCAGGCAATGGGCTCGGGTACGTGA
  GGCATGTTGACAATCCCCACGGCGATGGGCGCTGCATCACCTGTATCTATTACCTGAATCAGAACTGGGA
  TGTTAAGGTGCATGGCGGCCTGCTGCAGATCTTCCCTGAGGGTCGGCCAGTGGTAGCCAACATCGAGCCA
  CTCTTTGACCGGTTGCTCATTTTCTGGTCTGACCGACGGAATCCACATGAGGTGAAGCCAGCCTATGCCA
  CCAGGTACGCCATCACTGTCTGGTATTTTGATGCCAAGGAGCGGGCAGCAGCCAGAGACAAGTATCAGCT
  AGCATCGGGACAGAAAGGTGTTCAAGTACCCGTGTCACAGCCAGCTACACCTACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212035 representing NM_001004083
  Red=Cloning site Green=Tags(s)
  
  MDSPCQPQALNQALPQLPGSVSESLEPSRARMGVESYLPCPLLPSYHRSGASGEASAGNGTPRTTATATT
  TTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQE
  NQEAKGESGVGCDSGGGSSNSTTHSSGEASSRLREEAQPSAPERLALDYIVPCMRYYGICVKDNFLGAVL
  GGRVLAEVEALKWGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCAGRLG
  NYVINGRTKAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEP
  LFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPATPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004083
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004083.1, NP_001004083.1
• RefSeq Size: 2013 bp
• RefSeq ORF: 1248 bp
• Locus ID: 308457
• UniProt ID: Q6AYU4
• Cytogenetics: 1q21
• MW: 44.7 kDa
• Gene Summary: HIF-prolyl hydroxylase 1; might be involved in might play a role in hypoxic preconditioning [RGD, Feb 2006]