Ggt5 (NM_019235) Rat Tagged ORF Clone

CAT#: RR210284

Ggt5 (Myc-DDK-tagged ORF) - Rat gamma-glutamyltransferase 5 (Ggt5), (10 ug)

Product Images

Circular map for RR210284

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ggt5
• Synonyms: Ggta1; Ggtla1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210284 representing NM_019235
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATGGGGCCATAGGACCACAGTCTGCCTGGTCCTGCTGGGTGTGAGTCTGGGTCTGGCCATCATTG
  TGCTGGCTGTGGTCCTTCCTCATCACCAAGCCTCTTGCCGTCCCGATGCCTTCACTCGTGCTGCCGTAGC
  TGCTGATTCTAAGATCTGCTCAGATATTGGACGAGTCATCCTCCAGCAGCAGGGCTCACCTGTGGACGCC
  GCCATTGCTGCCCTCATCTGTACCGGTGTTGTTAACCCTCAGAGCATGGGCCTGGGCGGAGGGGTCGTCT
  TCACCATCTACAATGCATCTACAGGGAAGGTGGAGGTCATCAATGCTCGGGAGACAGTGCCAGCCAGCCA
  TGACCAGAGACTGCTAGACCAGTGTACAAATGCCCTGCCACTGTGCACAGGGGCCCAGTGGATCGGGGTT
  CCTGGTGAGCTCCGCGGCTATGCTGAGGCGCACCGCCGATACGGCCGCCTGCCTTGGGCTCAGCTTTTCC
  AGCCCACCATTGCCCTGCTCCGAGAGGGTTTCCGTGTGCCCCCTATCCTCAGTCAGTTCCTCAACACCAG
  CTTCCTGCAGCCCTGCTTGAATTCATCAACCCTGAGACAGCTCTTCTTCAATGGGACAGAAACCTTGAGA
  TCTCAGGACCCACTCCCATGGCCTGCGCTGGCCAACACCTTGGAGACTGTGGCTAAGGAGGGTGCAGAGG
  TCTTATACACCGGAAAGCTGGGACAAACGCTGGTGGAAGACATTGCCTGGCAAGGAAGCCAGCTGACGGT
  TCAGGACTTGGCTGCCTTCCGGCCGAAAGTGGTGGAACCCCTGGAGATGGCCCTAGGAAACTATACCCTG
  TACTCTCCACCGCCGCCGGCAGGGGGCGCTATTCTTAGCTTCATTCTCAACGTGCTGAAAGGGTTCAACT
  TCTCTGCAGAGACGGTGGCCGGGCCTGAAGGGAAGGTGAACATGTACCACCACCTTGTAGAGGCTCTCAA
  GTTCGCAGTAGGGCAGAGGTGGCGGCTATGGGACCCCTACAGCCACCCAGGGATACAGAACATCTCCCAG
  GACCTTCTCCGGGAGACTCTAGCCCAGCACATCCGCCAGCAGATTGATGGCCGTGGTGACCACCAGCTCA
  GCCATTACAACCTGTCCGGGGTCAGGGGCAACAGCATGGGCACGTCTCATGTGTCTGTGTTGGGAGAAGA
  CGGCAGCGCTGTGGCAGCCACCAGCACCATCAACACACCCTTTGGAGCGATGGTGTACTCCCCACGGACA
  GGCATCCTCCTCAACAACGAGCTACTAGACTTATGCTGGAGACACAAGCCAGGTTCAACGGTCACCCCTC
  CCCCTGTTCCAGGTGAGCAGCCACCATCATCCATGGTACCCTCCATCTTGATCAACGAAGTCCAGGGGTC
  GAAGCTGGTGATCGGTGGGGCTGGTGGGGAGCTTATCATCTCTGCTGTGACCCAGGCTATCGTGAATAAG
  CTGTGGCTTGGCTTTAGCCTGACAGACGCCATCGCAGCGCCCATCCTACATGTGAATAGCAAGGGCCACG
  TGGAATATGAGCCCAAGTTTAACCAGGAGGTTCGGAAGGGGCTACAGGACCGAGGCCAGAGCCAGAGCCA
  GAGCCAGAGGCCGGTTTTCCTGAATTCGGTCCAGGCTGTGTTTCAGGAAGGGCCCTGTGTCTATGCAGCA
  TCTGACCTGAGGAAGGCTGGAAAGGCTTCAGGATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210284 representing NM_019235
  Red=Cloning site Green=Tags(s)
  
  MAWGHRTTVCLVLLGVSLGLAIIVLAVVLPHHQASCRPDAFTRAAVAADSKICSDIGRVILQQQGSPVDA
  AIAALICTGVVNPQSMGLGGGVVFTIYNASTGKVEVINARETVPASHDQRLLDQCTNALPLCTGAQWIGV
  PGELRGYAEAHRRYGRLPWAQLFQPTIALLREGFRVPPILSQFLNTSFLQPCLNSSTLRQLFFNGTETLR
  SQDPLPWPALANTLETVAKEGAEVLYTGKLGQTLVEDIAWQGSQLTVQDLAAFRPKVVEPLEMALGNYTL
  YSPPPPAGGAILSFILNVLKGFNFSAETVAGPEGKVNMYHHLVEALKFAVGQRWRLWDPYSHPGIQNISQ
  DLLRETLAQHIRQQIDGRGDHQLSHYNLSGVRGNSMGTSHVSVLGEDGSAVAATSTINTPFGAMVYSPRT
  GILLNNELLDLCWRHKPGSTVTPPPVPGEQPPSSMVPSILINEVQGSKLVIGGAGGELIISAVTQAIVNK
  LWLGFSLTDAIAAPILHVNSKGHVEYEPKFNQEVRKGLQDRGQSQSQSQRPVFLNSVQAVFQEGPCVYAA
  SDLRKAGKASGY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019235
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019235.2, NP_062108.2
• RefSeq Size: 2636 bp
• RefSeq ORF: 1719 bp
• Locus ID: 29566
• UniProt ID: Q5PQV0
• Cytogenetics: 20p12
• MW: 61.6 kDa
• Gene Summary: involved in protecting the lungs from stress due to oxidation or other types of toxics [RGD, Feb 2006]