Wnt6 (NM_001108226) Rat Tagged ORF Clone

CAT#: RR210259

Wnt6 (Myc-DDK-tagged ORF) - Rat wingless-type MMTV integration site family, member 6 (Wnt6), (10 ug)

Product Images

Circular map for RR210259

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wnt6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210259 representing NM_001108226
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCGCCGGTGCCCTCCTGCCTCGGACTGCTGCTGCTGCTGCTCTTGTGCCCTGCGCACGTCGGTG
  GACTGTGGTGGGCCGTGGGCAGCCCCCTGGTCATGGATCCTACCAGCATCTGCAGGAAGGCCAGGAGGCT
  GGCAGGAAGGCAGGCTGAGCTGTGCCAGGCGGAGCCGGAAGTAGTGGCAGAGCTTGCCCGGGGCGCAAGA
  CTGGGGGTTCGAGAATGTCAGTTCCAGTTCCGTTTCCGACGCTGGAACTGCTCCAGTCACAGCAAGGCCT
  TTGGGCGCGTCCTGCAGCAGGACATTCGAGAGACAGCTTTTGTGTTTGCAATTACCGCTGCGGGTGCCAG
  TCACGCGGTCACTCAGGCCTGTTCCATGGGAGAGCTCCTACAGTGTGGTTGTCAGGCACCCCGCGGGCGC
  GCACCGCCTAGGCCCTCCGGCCTTCTGGGCACTCCTGGACCTCCGGGACCAACTGGCTCTCCAGATGCCA
  GCGCAGCCTGGGAGTGGGGAGGCTGCGGAGATGATGTCGACTTTGGGGATGAGAAGTCAAGACTCTTTAT
  GGATGCACAGCACAAGCGGGGACGTGGAGATATCCGTGCTTTGGTGCAACTGCATAACAACGAGGCAGGC
  AGACTGGCGGTGCGGAGCCACACGCGCACCGAGTGTAAGTGCCATGGGCTTTCGGGTTCCTGTGCTCTGC
  GCACCTGCTGGCAGAAGCTGCCACCGTTCCGCGAGGTGGGCGCACGGCTGCTGGAGCGCTTCCACGGCGC
  CTCACGCGTCATGGGCACCAACGACGGCAAAGCTCTGTTGCCTGCGGTCCGCACCCTCAAGCCTCCCGGC
  CGAGCCGATCTCCTCTACGCAGCCGATTCACCCGACTTCTGCGCCCCCAACCGGCGCACCGGTTCACCGG
  GCACGCGCGGCCGCGCCTGCAACAGCAGTGCCCCGGACCTTAGCGGCTGCGACCTGTTGTGCTGCGGTCG
  CGGACACCGCCAGGAGAGCGTACAGCTCGAGGAGAACTGCCTGTGCCGCTTCCACTGGTGCTGCGTGGTG
  CAATGCCACCGCTGCCGCGTGCGCAAGGAGCTCAGCCTGTGCCTC
  
  CAAGCTTAACTAGCTAGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATC
  TGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAAAC
  Protein Sequence

  >RR210259 representing NM_001108226
  Red=Cloning site Green=Tags(s)
  
  MLPPVPSCLGLLLLLLLCPAHVGGLWWAVGSPLVMDPTSICRKARRLAGRQAELCQAEPEVVAELARGAR
  LGVRECQFQFRFRRWNCSSHSKAFGRVLQQDIRETAFVFAITAAGASHAVTQACSMGELLQCGCQAPRGR
  APPRPSGLLGTPGPPGPTGSPDASAAWEWGGCGDDVDFGDEKSRLFMDAQHKRGRGDIRALVQLHNNEAG
  RLAVRSHTRTECKCHGLSGSCALRTCWQKLPPFREVGARLLERFHGASRVMGTNDGKALLPAVRTLKPPG
  RADLLYAADSPDFCAPNRRTGSPGTRGRACNSSAPDLSGCDLLCCGRGHRQESVQLEENCLCRFHWCCVV
  QCHRCRVRKELSLCL
  
  NNNNEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-HindIII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108226
• ORF Size: 1095 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108226.1, NP_001101696.1
• RefSeq Size: 2063 bp
• RefSeq ORF: 1098 bp
• Locus ID: 316526
• Cytogenetics: 9q33
• MW: 39.7 kDa