Jmjd6 (NM_001012143) Rat Tagged ORF Clone

CAT#: RR209542

Jmjd6 (Myc-DDK-tagged ORF) - Rat jumonji domain containing 6 (Jmjd6), (10 ug)

Product Images

Circular map for RR209542

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Jmjd6
• Synonyms: Ptdsr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209542 representing NM_001012143
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCACAAGAGCAAGAAGCGCATCCGCGAGGCCAAGCGGAGTGCGCGGCCCGAGCTCAAGGACTCGC
  TTGACTGGACGCGACACAATTACTATGAGAGCTACCCGCTGAGCCCTGCGGCCGTGCCGGATAACGTGGA
  GAGAGCTGATGCTTTGCAGCTGTCGGTGAAAGAATTTGTAGAGCGGTATGAAAGACCTTACAAGCCCGTG
  GTTCTGCTGAATGCACAAGAGGGCTGGTCCGCACAGGAGAAATGGACTCTGGAGCGTCTCAAAAGGAAAT
  ACCGGAACCAGAAGTTCAAATGCGGTGAGGATAATGACGGCTACTCGGTGAAGATGAAAATGAAATATTA
  CATCGAGTACATGGAGAGCACCCGCGATGACAGTCCCCTTTACATCTTTGATAGCAGCTATGGTGAACAC
  CCCAAAAGAAGGAAACTTTTGGAAGACTATAAGGTGCCCAAGTTTTTCACAGATGATCTTTTCCAATATG
  CGGGGGAGAAGCGCAGACCCCCTTACAGGTGGTTTGTGATGGGGCCACCACGTTCTGGAACTGGGATTCA
  CATTGACCCTCTGGGGACCAGTGCCTGGAATGCCTTAGTTCAGGGTCACAAGCGGTGGTGCCTGTTCCCA
  ACAAACACACCCCGAGAACTCATCAAGGTGACCCGAGAAGAAGGAGGAAACCAACAGGATGAAGCAATTA
  CCTGGTTTAATGTCATTTATCCCCGGACACAGCTTCCAACCTGGCCACCCGAATTCAAACCCCTGGAGAT
  ATTGCAGAAACCAGGAGAAACTGTCTTTGTACCAGGGGGCTGGTGGCACGTCGTCCTCAATCTTGACACC
  ACCATTGCCATCACCCAGAACTTTGCCAGCAGCACCAACTTCCCTGTTGTGTGGCACAAGACGGTAAGAG
  GGCGGCCAAAGTTATCCAGGAAGTGGTATAGGATCTTGAAGCAGGAGCACCCTGAGCTGGCAGTGCTTGC
  CGACGCTGTTGACCTCCAAGAGTCCACAGGCATCGCCTCTGACAGCTCCAGTGACTCTTCTAGCTCCTCC
  AGCTCCAGCTCGTCGGACTCAGACTCGGAGTGTGAATCTGGGTCAGAAGGTGATGGGACAACACATCGCA
  GGAAGAAGAGGAGAACTTGCAGCATGGTGGGAAATGGGGACACTACCTCACAGGATGACTGCGTGAGCAA
  AGAGCGCAGTTCCTCCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209542 representing NM_001012143
  Red=Cloning site Green=Tags(s)
  
  MNHKSKKRIREAKRSARPELKDSLDWTRHNYYESYPLSPAAVPDNVERADALQLSVKEFVERYERPYKPV
  VLLNAQEGWSAQEKWTLERLKRKYRNQKFKCGEDNDGYSVKMKMKYYIEYMESTRDDSPLYIFDSSYGEH
  PKRRKLLEDYKVPKFFTDDLFQYAGEKRRPPYRWFVMGPPRSGTGIHIDPLGTSAWNALVQGHKRWCLFP
  TNTPRELIKVTREEGGNQQDEAITWFNVIYPRTQLPTWPPEFKPLEILQKPGETVFVPGGWWHVVLNLDT
  TIAITQNFASSTNFPVVWHKTVRGRPKLSRKWYRILKQEHPELAVLADAVDLQESTGIASDSSSDSSSSS
  SSSSSDSDSECESGSEGDGTTHRRKKRRTCSMVGNGDTTSQDDCVSKERSSSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012143
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012143.2, NP_001012143.2
• RefSeq Size: 1730 bp
• RefSeq ORF: 1212 bp
• Locus ID: 360665
• UniProt ID: Q6AYK2
• Cytogenetics: 10q32.2
• MW: 46.5 kDa
• Gene Summary: Dioxygenase that can both act as a arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Regulates RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. Hydroxylates its own N-terminus, which is required for homooligomerization. In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which preferentially demethylates asymmetric dimethylation. Demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), including mono-, symmetric di- and asymmetric dimethylated forms, thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. In collaboration with BRD4, interacts with the positive transcription elongation factor b (P-TEFb) complex in its active form to regulate polymerase II promoter-proximal pause release for transcriptional activation of a large cohort of genes. On distal enhancers, so called anti-pause enhancers, demethylates both histone H4R3me2 and the methyl cap of 7SKsnRNA leading to the dismissal of the 7SKsnRNA:HEXIM1 inhibitor complex. After removal of repressive marks, the complex BRD4:JMJD6 attract and retain the P-TEFb complex on chromatin, leading to its activation, promoter-proximal polymerase II pause release, and transcriptional activation. Demethylates other arginine methylated-proteins such as ESR1. Has no histone lysine demethylase activity (By similarity). Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65 (By similarity). Seems to be necessary for the regulation of macrophage cytokine responses (By similarity).[UniProtKB/Swiss-Prot Function]