Cfb (NM_212466) Rat Tagged ORF Clone

CAT#: RR209436

Cfb (Myc-DDK-tagged ORF) - Rat complement factor B (Cfb), (10 ug)

Product Images

Circular map for RR209436

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cfb
• Synonyms: Bf; Da1-24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209436 representing NM_212466
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAATGGAGGGTCCCCAGCTCTGCTTAGTCCTCTTGGTCTTAGGCCTCTCCTCCGGAGGTGTGAGCG
  CAACTCCAGTGCTTGAGGCCCGGCCCCAGGTCTCTTGCTCTCTGGAGGGAGTAGAGATCAAAGGCGGCTC
  CTTCCAACTTCTCCAAGACGGTCAGGCCCTGGAGTACCTGTGTCCCTCTGGCTTCTACCCATACCCTGTG
  CAGACTCGAACCTGCAAATCCACAGGCTCCTGGAGTGTCCTCCAGACCCGGGACCAAAAGATTGTCAAGA
  AGGCAGAATGCAGAGCAATACGCTGCCCACGACCACAGGACTTTGAAAATGGGGAGTTCTGGCCCCGGTC
  CCCCTACTACAACCTGAGTGATCAGATTTCTTTTCAATGCTATGATGGCTACACTCTCCGGGGCTCTGCT
  AATCGCACCTGCCAAGAGAATGGCCGGTGGGATGGGCAAACAGCAATCTGTGATGATGGAGCGGGATACT
  GTCCCAACCCGGGTATTCCTATTGGGACAAGGAAGGTGGGAAGCCAGTACCGTCTTGAAGACACTGTCAC
  TTACCACTGTAGTCGGGGACTTGTCCTACGTGGCTCCCAGCAGCGAAGGTGCCAGGAAGGTGGCTCGTGG
  AGTGGGACAGAGCCTTCCTGCCAAGATTCCTTCATGTACGACAGCCCTCAAGAGGTGGCCGAAGCATTTC
  TATCCTCCCTGACAGAGACCATCGAAGGAGCAGATGCGGAGGATGGGCACAGCCCAGGGGAACAGCAGAA
  GAGGAAGATTATCCTGGACCCCTCGGGCTCCATGAATATCTACATGGTGCTGGATGGATCCGACAGCATC
  GGGGCCAGCAACTTCACAGGGGCCAAGCGGTGTCTCGCCAACTTGATTGAGAAGGTGGCGAGTTATGGGG
  TGAAGCCAAGATACGGTCTAGTGACATATGCCACAGTCCCCAAAGTCTTGGTCAGAGTGTCTGAGGAGAG
  GAGTAGTGATGCCGACTGGGTCACAGAGAAGCTCAACCAAATCAGTTATGAAGACCACAAGCTGAAGTCA
  GGGACCAACACCAAGAAGGCTCTCCAGGCTGTATACAGCATGATGAGCTGGCCAGGGGATGCTCCGCCTG
  AAGGCTGGAATCGAACCCGCCACGTCATCATCATCATGACTGATGGCTTGCACAACATGGGTGGAGACCC
  TGTCACTGTCATTGAGGACATCCGAGACTTGCTGGATATTGGCAGGGATCGCAAAAATCCCCGGGAGGAT
  TATTTGGATGTGTATGTGTTTGGGGTCGGGCCTCTGGTGGACCCTGTGAACATCAATGCCTTGGCTTCCA
  AAAAGAACAATGAGCAGCATGTGTTCAAGGTCAAGGACATGGAGGATCTGGAGAACGTCTTCTACAAAAT
  GATCGATGAAACCAAATCTCTGGGTCTCTGTGGCATGGTGTGGGAGCATCAGAAAGGCGGTGATTATTAC
  AAGCAACCATGGCAAGCCAAGATCTCAGTCACTCGTCCTCTGAAAGGACATGAGAACTGTATGGGGGCCG
  TGGTGTCCGAGTACTTCGTGCTGACAGCAGCGCATTGCTTCACAGTGGAAGATCAGAAACACTCCATCAA
  GGTCAACGTGGAGGGGAAAAGGCGGGACCTGGAGATTGAAGAGGTCCTGTTCCACCCTAATTACGACATC
  AATGGGAAAAAGGCAGAAGGAATCTCTGAGTTCTATGACTATGATGTTGCCCTCATCAAGCTCAAGACCA
  AGCTGAAGTACAGCCAGACTCTCAGGCCCATCTGTCTCCCCTGCACAGAGGGAACCACCCGAGCCTTGCG
  GCTTCCTCAGACAGCCACCTGCAAACAGCACAAGGAAGAGTTGCTCCCTATGAAGGACGTCAAAGCTCTG
  TTTGTATCCGAGGAAGGGAAGAAGCTGACCCGGAAGGAGGTGTACATCAAGAATGGGGAAAAGAAAGCCA
  GTTGTGAGAGAGATGCTACAAAGGCCCAAGGCTATGAGAAGGTCAAAGTTGCCTCTGAGGTGGTCACCCC
  CAGGTTCCTGTGCACCGGAGGGGTAGATCCCTATGCTGACCCCAACACATGCAAAGGAGACTCCGGGGGC
  CCTCTCATTGTTCACAAGAGAAGCCGCTTCATTCAAGTTGGTGTGATCAGCTGGGGAGTAGTGGATGTCT
  GCAAAGACCCGAGGCGGCAACAGTTGGTGCCCTCCTATGCCCGGGACTTCCACATCAATCTCTTCCAGGT
  GCTGCCCTGGCTAAAGGAGAAGCTCAAAGACGAGGACTTGGGTTTCTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209436 representing NM_212466
  Red=Cloning site Green=Tags(s)
  
  MTMEGPQLCLVLLVLGLSSGGVSATPVLEARPQVSCSLEGVEIKGGSFQLLQDGQALEYLCPSGFYPYPV
  QTRTCKSTGSWSVLQTRDQKIVKKAECRAIRCPRPQDFENGEFWPRSPYYNLSDQISFQCYDGYTLRGSA
  NRTCQENGRWDGQTAICDDGAGYCPNPGIPIGTRKVGSQYRLEDTVTYHCSRGLVLRGSQQRRCQEGGSW
  SGTEPSCQDSFMYDSPQEVAEAFLSSLTETIEGADAEDGHSPGEQQKRKIILDPSGSMNIYMVLDGSDSI
  GASNFTGAKRCLANLIEKVASYGVKPRYGLVTYATVPKVLVRVSEERSSDADWVTEKLNQISYEDHKLKS
  GTNTKKALQAVYSMMSWPGDAPPEGWNRTRHVIIIMTDGLHNMGGDPVTVIEDIRDLLDIGRDRKNPRED
  YLDVYVFGVGPLVDPVNINALASKKNNEQHVFKVKDMEDLENVFYKMIDETKSLGLCGMVWEHQKGGDYY
  KQPWQAKISVTRPLKGHENCMGAVVSEYFVLTAAHCFTVEDQKHSIKVNVEGKRRDLEIEEVLFHPNYDI
  NGKKAEGISEFYDYDVALIKLKTKLKYSQTLRPICLPCTEGTTRALRLPQTATCKQHKEELLPMKDVKAL
  FVSEEGKKLTRKEVYIKNGEKKASCERDATKAQGYEKVKVASEVVTPRFLCTGGVDPYADPNTCKGDSGG
  PLIVHKRSRFIQVGVISWGVVDVCKDPRRQQLVPSYARDFHINLFQVLPWLKEKLKDEDLGFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_212466
• ORF Size: 2289 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_212466.3, NP_997631.2
• RefSeq Size: 2573 bp
• RefSeq ORF: 2292 bp
• Locus ID: 294257
• Cytogenetics: 20p12
• MW: 85.4 kDa
• Gene Summary: predicted gene; component of the alternative pathway of complement activation; may be linked to the major histocompatibility complex and thus involved in the immune response [RGD, Feb 2006]