Lars (NM_001009637) Rat Tagged ORF Clone

CAT#: RR209318

Lars (Myc-DDK-tagged ORF) - Rat leucyl-tRNA synthetase (Lars), (10 ug)

Product Images

Circular map for RR209318

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lars
• Synonyms: MGC105585
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209318 representing NM_001009637
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAAGAAAAGGAACAGCCAAAGTAGACTTCTTGAAGAAGATTGAGAAAGAAGTCCAGCAGAAAT
  GGGAAGCGGAGAAAGTGTTTGAAGTCAGCGCGTCTTCACTGGAGAAGCAGAAGCAGAGCAGCAAGGGCAA
  ATACTTTGTCACCTTTCCATATCCATACATGAATGGACGCCTTCATTTGGGACACACGTTTTCCTTATCC
  AAGTGTGAGTTTGCAGTAGGATACCAGAGATTAAAAGGAAAAAGCTGTCTGTTCCCCTTCGGGTTACACT
  GTACCGGTATGCCTATTAAGGCATGTGCTGACAAGCTGAAAAGGGAGATAGAGCTGTATGGCTGCCCTCC
  TGATTTTCCAGAGGAGGAGGAGGAAGAGGAAGAAAGCAGTGCTAAACCTGGAGATATAGTAATGAAGGAT
  AAAGCCAAAGGGAAAAAGAGTAAAGCCGCTGCTAAAGCAGGATCTTCTAAGTACCAGTGGAACATTATGA
  AATCCCTTGGGCTCTCTGATGAAGACATCGCGAAGTTTTCTGAAGCAGAACATTGGCTTGATTATTTCCC
  ACCACTGGCTGTTCAGGATCTGAAGAAAATAGGTTTGAAGGTAGACTGGCGGCGTTCCTTCATCACCACG
  GATGTGAATCCTTACTATGATTCTTTTGTCAGGTGGCAATTTTTAACTTTAAGAGAAAGAAATAAAATTA
  AATTCGGGAAGCGGTACACTATTTATTCCCCAAAAGATGGACAGCCTTGCATGGATCATGACAGACAAAC
  CGGAGAGGGTGTTGGACCTCAGGAATATACCCTAGTCAAACTGAAGGTGCTTGATCCATACCCATCAAAA
  TTAAGTGGCCTGAAAGGGAAAAATATCTTCCTAGTAGCTGCCACACTCAGACCTGAGACTATGTTTGGAC
  AGACAAATTGTTGGGTTCGGCCTGATATGAAATACATTGGGTTTGAGACAGCAAATGGAGACATATTCAT
  CTGTACCCAGAGAGCAGCCAGGAATATGTCATACCAAGGCTTCACCAAGCACAATGGCGTGGTGCCGGTT
  GTGAAGGAGTTAATGGGAGAGGAAATCCTTGGGGCTTCACTTTCTGCACCCCTGACATGCTATAAGGTGA
  TCTATGTTCTCCCTATGCTGACCATTAAGGAGGATAAAGGCACTGGGGTGGTCACAAGCGTTCCTTCTGA
  TTCCCCGGATGATTTTGCGGCACTCAGAGACTTGAAGAAAAAGCAAGCCTTACGAGCAAAGTATGCAGTC
  AGAGATGACATGGTCCTGCCCTTTGAACCGGTGCCAGTCCTTGAAATCCCAGGGATTGGAAATCTTCCTG
  CTGTGACCGTTTGTGACGAACTGAAAATTCAGAGCCAGAATGACCGTGAAAAACTTGCAGAGGCAAAGGA
  GAAATTGTATCTAAGAGGATTTTATGATGGTGTAATGCTAGTGGATGGATTTAAAGGGCAGAAGATCCAA
  CATGTAAAGAAGACTATTCAGAAAAACATGATTGATGCTGGAGATGCGCTTATCTACATGGAGCCAGAAA
  AACAAGTGGTGTCGAGGTCTGCAGATGAGTGTGTTGTGGCCCTGTGTGACCAATGGTACTTGGATTATGG
  AGATGAGAACTGGAAGAAACAGACATTTCAGTGCCTGAAGAACATGGAAACATTCTGCGAGGAGAGCAGG
  AAGAATTTTGAAGCTTCCTTAGACTGGCTGCAAGAACATGCCTGCTCAAGAACTTACGGTTTAGGCACGC
  GTCTGCCTTGGGATGAGCAGTGGTTGATTGAATCCCTCTCAGACTCCACTATTTACATGGCGTTTTACAC
  AGTTGCACACCTGTTGCAGGGGGGTGACCTGCAGGGTCAGGCGGAGTCTCCACTGGGCATCAGACCCCAG
  CAGATGACGAGGGATGTTTGGGATTATGTGTTCTTCAAGGATGCTCCATTTCCCAAGACTCAGATCCCAA
  AGGAAAAGCTGGATCAGCTGAAGCAGGAATTTGAGTTTTGGTATCCAGTGGACCTTCGAGCCTCTGGCAA
  GGATCTGATTCCAAATCATCTTTCATATTACATTTACAATCATGTGGCCATGTGGCCAGAACAAAGCGAC
  AAGTGGCCTGTATCCGTTCGAGCAAATGGACACCTGCTGCTCAACTCCGAGAAGATGTCAAAATCTACGG
  GAAACTTCCTCACTTTGAGCCAAGCTGTTGACAAGTTTTCCGCTGATGGAATGCGCCTGGCTCTGGCTGA
  TGCTGGCGACACTGTTGAAGATGCTAACTTTGTGGAAGCTATGGCAGACGCAGGCATTCTCCGTCTGTAC
  ACATGGGTAGAGTGGGTCAAAGAAATGCTTGCCAACTGCAGCAGCCTGAGAAGTGGACCTGCCAACTCAT
  TCAATGATCGTGTGTTTGCCAGTGAAATGAATGCAGGAATTATAAAAACAGATCAAAACTATGAAAAGAT
  GATGTTTAAAGAAGCGTTGAAAACAGGTTTTTTTGAGTTTCAGGCTGCGAAAGACAAGTACCGTGAACTG
  GCCACAGAGGGGATGCACAGAGAGCTTGTGTTCCGGTTTATTGAAGTTCAAACAATCCTTCTGACTCCAT
  TCTGTCCACATTTGTGTGAGCACATCTGGACACTGCTGGGAAAGCCTGATTCAATTATGCATGCCTCATG
  GCCTGTGGCAGGTCCCGTGGATGAATCCCTGATTCGTTCCTCACAGTACCTCATGGAAGTAGCACACGAC
  CTTAGATTACGGCTCAAGAATTACATGACGCCAGCTAAAGGGAAGAAGACGGACAAGCAACCAGTTCAGA
  GGCCCTCACACTGCACCATCTATGTGGCAAAGAACTACCCTGTCTGGCAGCACATTACCCTGACAACCCT
  ACGGAGCCACTTTGAGGCTAACAATGGAAAATTGCCTGATAACAAAGTCATCGCTTGTGAACTAGGCAGT
  TTGCCAGAACTGAAGAAATATATGAAGAAAGTTATGCCATTTGTTGCCATGATTAAGGAAAACATGGAGA
  AGAAAGGGCCGCGAGTCTTGGATTTAGAGTTGGAATTTGATGAGCAGGCAGTCCTTATGGAGAATATAGT
  CTACCTAACCAATTCTCTTGAGCTGGAACATATAGACGTCAAGTTTGCCTCTGAAGCTGAAGATAAAGTC
  AGGGAAGAGTGCTGTCCTGGGAAGCCACTTAATGTTTTCAGAACAGAACCTGGTGTGCCAGTTTCCCTAG
  TAAATCCTCAGCCATCCAGCGGGCACTTCTCTACCAAAATCGACATCAGACAAGGAGATAGCTGTGAGTC
  CATAATCAGACGCCTGACGAAAATGGACCGAGGAATCAAAGATCTCTCCAAAGTGAAACTCATGCGCTTT
  GATGATCCTCTGTTGGGGCCTCGACGAGTTCCAGTCCTGGGGAGAGAGCAGAACGAGAAGACACTCATTT
  CTGAGAATGCCATTTTCCACGTGGACCTAGTGAGCAAGAAAGTTCACCTTACTGAGAACGGGCTGCGCGC
  AGACATCGGTGACACAATAGTCTATCTGGTTCAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209318 representing NM_001009637
  Red=Cloning site Green=Tags(s)
  
  MAERKGTAKVDFLKKIEKEVQQKWEAEKVFEVSASSLEKQKQSSKGKYFVTFPYPYMNGRLHLGHTFSLS
  KCEFAVGYQRLKGKSCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPEEEEEEEESSAKPGDIVMKD
  KAKGKKSKAAAKAGSSKYQWNIMKSLGLSDEDIAKFSEAEHWLDYFPPLAVQDLKKIGLKVDWRRSFITT
  DVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLVKLKVLDPYPSK
  LSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETANGDIFICTQRAARNMSYQGFTKHNGVVPV
  VKELMGEEILGASLSAPLTCYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDFAALRDLKKKQALRAKYAV
  RDDMVLPFEPVPVLEIPGIGNLPAVTVCDELKIQSQNDREKLAEAKEKLYLRGFYDGVMLVDGFKGQKIQ
  HVKKTIQKNMIDAGDALIYMEPEKQVVSRSADECVVALCDQWYLDYGDENWKKQTFQCLKNMETFCEESR
  KNFEASLDWLQEHACSRTYGLGTRLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGDLQGQAESPLGIRPQ
  QMTRDVWDYVFFKDAPFPKTQIPKEKLDQLKQEFEFWYPVDLRASGKDLIPNHLSYYIYNHVAMWPEQSD
  KWPVSVRANGHLLLNSEKMSKSTGNFLTLSQAVDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLY
  TWVEWVKEMLANCSSLRSGPANSFNDRVFASEMNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYREL
  ATEGMHRELVFRFIEVQTILLTPFCPHLCEHIWTLLGKPDSIMHASWPVAGPVDESLIRSSQYLMEVAHD
  LRLRLKNYMTPAKGKKTDKQPVQRPSHCTIYVAKNYPVWQHITLTTLRSHFEANNGKLPDNKVIACELGS
  LPELKKYMKKVMPFVAMIKENMEKKGPRVLDLELEFDEQAVLMENIVYLTNSLELEHIDVKFASEAEDKV
  REECCPGKPLNVFRTEPGVPVSLVNPQPSSGHFSTKIDIRQGDSCESIIRRLTKMDRGIKDLSKVKLMRF
  DDPLLGPRRVPVLGREQNEKTLISENAIFHVDLVSKKVHLTENGLRADIGDTIVYLVH
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009637
• ORF Size: 3534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009637.1, NP_001009637.1
• RefSeq Size: 3834 bp
• RefSeq ORF: 3537 bp
• Locus ID: 291624
• Cytogenetics: 18p11
• MW: 134.3 kDa