Acadm (NM_016986) Rat Tagged ORF Clone

CAT#: RR209147

Acadm (Myc-DDK-tagged ORF) - Rat acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain (Acadm), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR209147

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acadm
• Synonyms: MCAD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209147 representing NM_016986
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAGCGCTCCGCAGAGGCTACAAGGTCCTGAGAAGTGTCTCTCATTTTGAGTGTCGAGCACAAC
  ACACAAAACCATCTCTCAAGCAGGAGCCGGGACTAGGGTTTAGCTTCGAGTTGACGGAGCAGCAGAAAGA
  GTTTCAAACAATTGCTCGGAAGTTTGCCAGAGAGGAAATAATCCCGGTCGCCCCAGACTACGATAAAAGC
  GGGGAATACCCGTTCCCTCTCATCAAGAGAGCCTGGGAACTTGGGTTGATCAACACACACATTCCGGAGA
  GTTGTGGTGGTCTTGGCCTGGGAACTTTTGATGCGTGTTTAATTACGGAAGAGTTGGCATATGGGTGTAC
  AGGGGTGCAGACTGCTATTGAAGCAAATTCTTTGGGGCAAATGCCTGTGATTATTGCTGGAAATGATCAA
  CAGAAGAAGAAGTATTTGGGGAGGATGACGGAGCAGCTGATGATGTGTGCCTACTGCGTGACAGAACCCT
  CAGCAGGCTCTGATGTGGCGGGCATTAAGACCAAAGCAGAGAAGAAGGGTGATGAATATGTCATCAATGG
  CCAGAAGATGTGGATAACCAACGGGGGAAAGGCCAACTGGTATTTTGTATTGACGCGATCTAACCCAGAT
  CCTAAAGTACCTGCTAGTAAAGCCTTCACCGGATTCATCGTGGAGGCCGACACCCCGGGAATACACATCG
  GAAAAAAGGAACTAAACATGGGTCAGCGGTGCTCTGACACCAGAGGAATCACCTTCGAAGATGTCAGAGT
  GCCTAAGGAAAATGTGTTAATTGGTGAAGGAGCAGGTTTCAAGATTGCAATGGGGGCTTTTGATAGAACC
  AGGCCGACGGTCGCAGCTGGTGCTGTCGGGCTAGCCCAGAGAGCCCTGGACGAAGCTACTAAGTATGCCC
  TGGACAGGAAAACATTTGGAAAGCTGCTAGTGGAGCACCAAGGAGTTTCATTTCTGCTCGCAGAAATGGC
  GATGAAAGTTGAACTGGCCAGACTCAGTTACCAGCGAGCAGCCTGGGAGGTTGACTCCGGCCGCCGGAAC
  ACGTACTTTGCCTCTATTGCGAAGGCCTTTGCTGGAGATATTGCCAACCAGCTCGCTACCGATGCTGTGC
  AGATTTTCGGAGGCTATGGATTCAACACTGAGTACCCAGTAGAAAAGCTGATGCGGGACGCCAAGATCTA
  TCAGATTTACGAAGGTACTGCACAAATTCAGAGGCTGATCATAGCTCGTGAGCACATTGAAAAGTATAAA
  AAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209147 representing NM_016986
  Red=Cloning site Green=Tags(s)
  
  MAAALRRGYKVLRSVSHFECRAQHTKPSLKQEPGLGFSFELTEQQKEFQTIARKFAREEIIPVAPDYDKS
  GEYPFPLIKRAWELGLINTHIPESCGGLGLGTFDACLITEELAYGCTGVQTAIEANSLGQMPVIIAGNDQ
  QKKKYLGRMTEQLMMCAYCVTEPSAGSDVAGIKTKAEKKGDEYVINGQKMWITNGGKANWYFVLTRSNPD
  PKVPASKAFTGFIVEADTPGIHIGKKELNMGQRCSDTRGITFEDVRVPKENVLIGEGAGFKIAMGAFDRT
  RPTVAAGAVGLAQRALDEATKYALDRKTFGKLLVEHQGVSFLLAEMAMKVELARLSYQRAAWEVDSGRRN
  TYFASIAKAFAGDIANQLATDAVQIFGGYGFNTEYPVEKLMRDAKIYQIYEGTAQIQRLIIAREHIEKYK
  N
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016986
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016986.2, NP_058682.2
• RefSeq Size: 1954 bp
• RefSeq ORF: 1266 bp
• Locus ID: 24158
• UniProt ID: P08503
• Cytogenetics: 2q45
• MW: 46.6 kDa
• Gene Summary: catalyzes the alpha, beta-dehydrogenation of acyl-CoA esters in fatty acid metabolism [RGD, Feb 2006]