Ehhadh (NM_133606) Rat Tagged ORF Clone

CAT#: RR208983

Ehhadh (Myc-DDK-tagged ORF) - Rat enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase (Ehhadh), (10 ug)

Product Images

Circular map for RR208983

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ehhadh
• Synonyms: Lbp; MEF; Mfe; Mfe1; Pbe; Pbfe; pe-CoA; perMFE-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208983 representing NM_133606
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGTATCTGAGGCTGCCCCACTCCCTGGCGATGATCCGCCTCTGCAACCCGCCAGTCAACGCCG
  TCAGTCCAACTGTAATCAGGGAAGTAAGAAATGGACTCCAGAAAGCTGGATCAGACCACACGGTTAAAGC
  CATTGTGATCTGTGGCGCAAACGGAAACTTCTGTGCAGGTGCTGATATCCATGGCTTTAGTGCTTTTACC
  CCTGGCCTTGCATTAGGAAGCTTGGTAGATGAAATACAGAGATACCAGAAGCCGGTGTTGGCCGCTATCC
  AAGGTGTGGCTCTCGGAGGAGGACTGGAGCTGGCCTTGGGCTGTCACTATCGGATTGCCAATGCAAAGGC
  TCGTGTCGGCTTGCCAGAAGTCACACTGGGGATTCTTCCTGGTGCAAGAGGAACCCAGCTTCTCCCCAGG
  GTCGTTGGAGTTCCTGTTGCTCTTGACCTAATTACCTCAGGAAAATATCTTTCAGCAGATGAAGCACTCA
  GGCTTGGAATTCTGGATGCAGTCGTGAAGTCAGACCCAGTTGAAGAAGCCATCAAATTTGCTCAGAAGAT
  TATAGATAAACCCATAGAACCCCGCAGGATCTTTAACAAGCCAGTTCCAAGCTTGCCCAACATGGACAGT
  GTTTTTGCAGAAGCCATCGCCAAGGTACGAAAACAGTACCCTGGTGTCCTGGCTCCGGAGACGTGTGTCC
  GCTCAATCCAGGCCTCTGTGAAGCATCCCTACGAAGTAGGCATCAAGGAAGAGGAAAAGCTGTTTATGTA
  CCTCCGGGCATCCGGGCAGGCTAAAGCCCTACAGTATGCCTTCTTTGCTGAAAAGTCTGCAAATAAGTGG
  TCAACTCCCTCAGGAGCGTCTTGGAAAACAGCCTCTGCTCAACCCGTCTCCTCAGTTGGCGTTCTTGGCT
  TGGGAACGATGGGCCGAGGCATCGCCATTTCTTTTGCGAGAGTGGGGATCTCTGTGGTTGCTGTGGAGTC
  AGACCCAAAGCAGCTAGATGCTGCAAAGAAGATAATCACTTTCACCTTGGAGAAGGAAGCATCCAGAGCG
  CATCAGAACGGCCAAGCTTCGGCAAAACCAAAACTCAGGTTCTCCTCATCCACAAAGGAACTTTCAACTG
  TGGATTTGGTGGTTGAAGCAGTGTTCGAAGACATGAACCTGAAGAAAAAGGTCTTTGCTGAGCTGTCAGC
  CCTGTGCAAGCCAGGAGCCTTTCTGTGCACCAATACCTCGGCGCTGAACGTGGACGACATTGCTTCTTCC
  ACAGATCGCCCTCAGCTGGTGATTGGCACCCACTTCTTCTCACCAGCCCATGTCATGAGGTTGCTAGAGG
  TCATTCCTAGCCGATACTCTTCCCCTACTACCATCGCCACGGTTATGAGCTTGTCCAAAAAGATCGGAAA
  GATTGGAGTAGTGGTTGGCAACTGCTATGGATTTGTTGGGAATCGGATGTTGGCTCCCTATTACAACCAG
  GGGTTTTTCTTGTTAGAGGAAGGTAGCAAGCCAGAGGATGTAGACGGGGTCTTGGAAGAGTTTGGTTTTA
  AAATGGGACCCTTCAGAGTGTCAGACCTCGCAGGGCTAGATGTGGGTTGGAAAATTCGCAAGGGGCAAGG
  CCTTACTGGACCATCATTGCCACCAGGGACCCCCGTCCGAAAGAGGGGCAACAGCAGGTACTCCCCACTT
  GGCGATATGCTCTGTGAAGCTGGGCGGTTTGGTCAGAAGACAGGTAAAGGCTGGTATCAGTATGACAAGC
  CACTGGGTCGCATCCACAAACCTGATCCCTGGCTTTCTACGTTCCTGTCACAATATAGAGAGGTTCACCA
  CATCGAGCAGCGCACCATCAGCAAGGAGGAGATCCTGGAGCGTTGCTTATATTCCCTCATCAATGAGGCG
  TTCCGCATCTTGGAGGAGGGGATGGCTGCTCGCCCAGAGCACATTGATGTCATCTACTTGCACGGGTACG
  GGTGGCCAAGGCACAAGGGCGGGCCCATGTTCTATGCTGCCTCAGTTGGGTTGCCCACAGTTCTAGAGAA
  ACTGCAGAAATATTACAGGCAGAACCCTGACATCCCCCAGCTGGAGCCCAGTGACTACCTCAGAAGGCTG
  GTAGCCCAGGGAAGCCCTCCTCTGAAGGAATGGCAAAGCTTGGCAGGGCCCCACGGCAGCAAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208983 representing NM_133606
  Red=Cloning site Green=Tags(s)
  
  MAEYLRLPHSLAMIRLCNPPVNAVSPTVIREVRNGLQKAGSDHTVKAIVICGANGNFCAGADIHGFSAFT
  PGLALGSLVDEIQRYQKPVLAAIQGVALGGGLELALGCHYRIANAKARVGLPEVTLGILPGARGTQLLPR
  VVGVPVALDLITSGKYLSADEALRLGILDAVVKSDPVEEAIKFAQKIIDKPIEPRRIFNKPVPSLPNMDS
  VFAEAIAKVRKQYPGVLAPETCVRSIQASVKHPYEVGIKEEEKLFMYLRASGQAKALQYAFFAEKSANKW
  STPSGASWKTASAQPVSSVGVLGLGTMGRGIAISFARVGISVVAVESDPKQLDAAKKIITFTLEKEASRA
  HQNGQASAKPKLRFSSSTKELSTVDLVVEAVFEDMNLKKKVFAELSALCKPGAFLCTNTSALNVDDIASS
  TDRPQLVIGTHFFSPAHVMRLLEVIPSRYSSPTTIATVMSLSKKIGKIGVVVGNCYGFVGNRMLAPYYNQ
  GFFLLEEGSKPEDVDGVLEEFGFKMGPFRVSDLAGLDVGWKIRKGQGLTGPSLPPGTPVRKRGNSRYSPL
  GDMLCEAGRFGQKTGKGWYQYDKPLGRIHKPDPWLSTFLSQYREVHHIEQRTISKEEILERCLYSLINEA
  FRILEEGMAARPEHIDVIYLHGYGWPRHKGGPMFYAASVGLPTVLEKLQKYYRQNPDIPQLEPSDYLRRL
  VAQGSPPLKEWQSLAGPHGSKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133606
• ORF Size: 2166 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133606.1, NP_598290.1
• RefSeq Size: 3097 bp
• RefSeq ORF: 2169 bp
• Locus ID: 171142
• UniProt ID: P07896
• Cytogenetics: 11q23
• MW: 78.7 kDa
• Gene Summary: enzyme involved in the B-oxidation cycle of peroxisomes [RGD, Feb 2006]