Gmppa (NM_001025056) Rat Tagged ORF Clone

CAT#: RR208007

Gmppa (Myc-DDK-tagged ORF) - Rat GDP-mannose pyrophosphorylase A (Gmppa), (10 ug)

Product Images

Circular map for RR208007

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gmppa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208007 representing NM_001025056
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAAAGCTGTGATCCTCATTGGAGGACCCCAGAAGGGGACTCGCTTCAGGCCTTTGTCTTTTGAAG
  TGCCCAAACCGTTGTTCCCTGTGGCAGGTGTCCCTATGATCCAGCACCATATAGAAGCCTGTGCCCAGGT
  CCCTGGGATGCAGGAGATTCTACTCATTGGCTTCTACCAGCCTGATGAGGCTCTCACCCAGTTCCTGGAA
  GCTGCACAGCAGGAGTTTAACCTTCCAGTCAGGTATCTGCAGGAGTTCACTCCCCTTGGCACAGGGGGTG
  GCCTCTACCATTTTCGGGACCAGATCCTGGCTGGCGCACCTGAGGCCTTCTTCGTGCTCAATGCTGATGT
  CTGTTCTGACTTCCCTCTGAGTGCCATGTTGGATGCTCACAGGCTCCAACGCCACCCTTTCCTACTCCTT
  GGCACCACGGCGAACAGGACACAATCTCTCAACTACGGCTGCATCGTTGAGAATCCACAGACGCATGAGG
  TCCTGCACTATGTGGAGAAACCCAGCACCTTTATCAGCGACATCATCAACTGTGGCATCTACCTTTTCTC
  CCCAGAAGCCCTGAAGCCTCTCCGGGATGTTTTCCAGCGTAATCAGCAGGATGGGCAACTGGAGGAATCT
  CCAGGCTCCTGGCCTGGTGCAGGGACCATCCGACTAGAGCAGGATGTGTTCTCAGCCCTGGCTGGGCAGG
  GCCAAATCTATGTGCACCTCACCGACGGCATCTGGAGCCAGATCAAGTCGGCAGGCTCGGCCCTTTATGC
  TTCCCGCCTGTATCTGGGGCGATACCAGATCACCCACCCAGAACGGCTAGCCAGACACACTGCAGGGGGC
  CCACGAATCAGAGGAAATGTTTACATCCATCCAACTGCTAAAGTGGCCCCATCAGCTGTGCTGGGCCCCA
  ATGTCTCCATTGGGAAGGGGGTGACCATAGGCGAGGGTGTGCGTCTGCGGGAGAGTATTGTCCTCCATGG
  AGCCACACTGCAGGAACACACCTGTGTCCTTCACAGCATTGTGGGCTGGGGGAGCACTGTGGGGCGCTGG
  GCCCGCGTAGAGGGGACTCCCAATGACCCCAATCCCAATGACCCCCGCGCCCGCATGGACAGCGAGAGCC
  TCTTCAAAGATGGAAAGCTACTTCCTGCCATCACCATCCTGGGCTGCCGAGTACGGATCCCCGCTGAGGT
  GCTTATCTTGAACTCGATTGTTCTACCACATAAGGAGCTGAGTCGAAGCTTCACCAACCAAATCATTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208007 representing NM_001025056
  Red=Cloning site Green=Tags(s)
  
  MLKAVILIGGPQKGTRFRPLSFEVPKPLFPVAGVPMIQHHIEACAQVPGMQEILLIGFYQPDEALTQFLE
  AAQQEFNLPVRYLQEFTPLGTGGGLYHFRDQILAGAPEAFFVLNADVCSDFPLSAMLDAHRLQRHPFLLL
  GTTANRTQSLNYGCIVENPQTHEVLHYVEKPSTFISDIINCGIYLFSPEALKPLRDVFQRNQQDGQLEES
  PGSWPGAGTIRLEQDVFSALAGQGQIYVHLTDGIWSQIKSAGSALYASRLYLGRYQITHPERLARHTAGG
  PRIRGNVYIHPTAKVAPSAVLGPNVSIGKGVTIGEGVRLRESIVLHGATLQEHTCVLHSIVGWGSTVGRW
  ARVEGTPNDPNPNDPRARMDSESLFKDGKLLPAITILGCRVRIPAEVLILNSIVLPHKELSRSFTNQIIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025056
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025056.1, NP_001020227.1
• RefSeq Size: 1662 bp
• RefSeq ORF: 1263 bp
• Locus ID: 501167
• UniProt ID: Q5XIC1
• Cytogenetics: 9q33
• MW: 46.2 kDa
• Gene Summary: May serve as a regulatory subunit and allow allosteric feedback inhibition of GMPPB by GDP-mannose.[UniProtKB/Swiss-Prot Function]