Drd2 (NM_012547) Rat Tagged ORF Clone

CAT#: RR207803

Drd2 (Myc-DDK-tagged ORF) - Rat dopamine receptor D2 (Drd2), (10 ug)

Product Images

Circular map for RR207803

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Drd2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207803 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCACTGAACCTGTCCTGGTACGATGACGATCTGGAGAGGCAGAACTGGAGCCGGCCCTTCAATG
  GGTCAGAAGGGAAGGCAGACAGGCCCCACTACAACTACTATGCCATGCTGCTCACCCTCCTCATCTTTAT
  CATCGTCTTTGGCAATGTGCTGGTGTGCATGGCTGTATCCCGAGAGAAGGCTTTGCAGACCACCACCAAC
  TACTTGATAGTCAGCCTTGCTGTGGCTGATCTTCTGGTGGCCACACTGGTAATGCCGTGGGTTGTCTACC
  TGGAGGTGGTGGGTGAGTGGAAATTCAGCAGGATTCACTGTGACATCTTTGTCACTCTGGATGTCATGAT
  GTGCACAGCAAGCATCCTGAACCTGTGTGCCATCAGCATTGACAGGTACACAGCTGTGGCAATGCCCATG
  CTGTATAACACACGCTACAGCTCCAAGCGCCGAGTTACTGTCATGATTGCCATTGTCTGGGTCCTGTCCT
  TCACCATCTCCTGCCCACTGCTCTTCGGACTCAACAATACAGACCAGAATGAGTGTATCATTGCCAACCC
  TGCCTTTGTGGTCTACTCCTCCATTGTCTCATTCTACGTGCCCTTCATCGTCACTCTGCTGGTCTATATC
  AAAATCTACATCGTCCTCCGGAAGCGCCGGAAGCGGGTCAACACCAAGCGCAGCAGTCGAGCTTTCAGAG
  CCAACCTGAAGACACCACTCAAGGGCAACTGTACCCACCCTGAGGACATGAAACTCTGCACCGTTATCAT
  GAAGTCTAATGGGAGTTTCCCAGTGAACAGGCGGAGAATGGATGCTGCCCGCCGAGCTCAGGAGCTGGAA
  ATGGAGATGCTGTCAAGCACCAGTCCCCCAGAGAGGACCCGGTATAGCCCCATCCCTCCCAGTCACCACC
  AGCTCACTCTCCCTGATCCATCCCACCACGGCCTACATAGCAACCCTGACAGTCCTGCCAAACCAGAGAA
  GAATGGGCACGCCAAGATTGTCAATCCCAGGATTGCCAAGTTCTTTGAGATCCAGACCATGCCCAATGGC
  AAAACCCGGACCTCCCTTAAGACGATGAGCCGCAGAAAGCTCTCCCAGCAGAAGGAGAAGAAAGCCACTC
  AGATGCTTGCCATTGTTCTCGGTGTGTTCATCATCTGCTGGCTGCCCTTCTTCATCACGCACATCCTGAA
  TATACACTGTGATTGCAACATCCCACCAGTCCTCTACAGCGCCTTCACATGGCTGGGCTATGTCAACAGT
  GCCGTCAACCCCATCATCTACACCACCTTCAACATCGAGTTCCGCAAGGCCTTCATGAAGATCTTGCACT
  GC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207803 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDPLNLSWYDDDLERQNWSRPFNGSEGKADRPHYNYYAMLLTLLIFIIVFGNVLVCMAVSREKALQTTTN
  YLIVSLAVADLLVATLVMPWVVYLEVVGEWKFSRIHCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPM
  LYNTRYSSKRRVTVMIAIVWVLSFTISCPLLFGLNNTDQNECIIANPAFVVYSSIVSFYVPFIVTLLVYI
  KIYIVLRKRRKRVNTKRSSRAFRANLKTPLKGNCTHPEDMKLCTVIMKSNGSFPVNRRRMDAARRAQELE
  MEMLSSTSPPERTRYSPIPPSHHQLTLPDPSHHGLHSNPDSPAKPEKNGHAKIVNPRIAKFFEIQTMPNG
  KTRTSLKTMSRRKLSQQKEKKATQMLAIVLGVFIICWLPFFITHILNIHCDCNIPPVLYSAFTWLGYVNS
  AVNPIIYTTFNIEFRKAFMKILHC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012547
• ORF Size: 1332 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012547.1, NP_036679.1
• RefSeq Size: 2750 bp
• RefSeq ORF: 1335 bp
• Locus ID: 24318
• UniProt ID: P61169
• Cytogenetics: 8q23
• MW: 50.9 kDa
• Gene Summary: mediates G-protein coupled signaling; may be involved in movement disorders, schizophrenia, and drug addiction [RGD, Feb 2006]