Tars2 (NM_001014040) Rat Tagged ORF Clone

CAT#: RR207239

Tars2 (Myc-DDK-tagged ORF) - Rat threonyl-tRNA synthetase 2, mitochondrial (putative) (Tars2), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR207239

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tars2
• Synonyms: RGD1308283; Tarsl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207239 representing NM_001014040
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCTCTGTCTGAGGTGGCGCCGGCTTGGGTTCCCACTCCCGGGGTTCCGCCGCTGCGAGCTCCACA
  CAGTGCGTGAGGCCCCTATACCAACTCCTCCACATTGGTTGGCAGAACGATTTGGCCTTTTTGAGGAGCT
  ATGGACTGCTCAGGTGAAGAGATTAGCAAGTATGACACAGAAGAAAGCCCGGACTATTAAGATATCACTT
  CCTGAAGGCCAGAAGGTAGATGCTGTTGCATGGAATACAACCCCTTATCAACTGGCCCAGCAGATCAGTT
  CGACACTGGCTGATACTGCAGTGGCTGCTGAAGTAAATGGAGAACTTTACGATCTGGACCGACCCTTGGA
  GACAGATTGTCACCTCAGATTTCTGACATTTGATTCCCCAGAGGGCAAAGCGGTGTTCTGGCGCTCTAGT
  GCCCACGTTCTGGGTGCAGCAGCTGAGCAACATCTGGGTGCTGTTCTCTGCAGAGGTCCAAGTACAGAAT
  CTGGCTTTTACCTTGATTTCTTCCTGGGAAAAGAACGGACAGTCCGCAGCACGGAGTTGCCCACTTTAGA
  GCGGATTTGCCAGGAGATCATAACTGCTGCACAGCCTTTCCGAAGGCTAGAGGCTTCCCGGGGTCAGCTT
  CGCCAGCTCTTCAAGGATAACCACTTTAAGCTTCATGTGATCGAGGAGAAAGTGACAGGCACGACAGCAA
  CAGTGTACGGGTGTGGCATGTCGGTTGACCTGTGCCAGGGCCCCCATCTTCGGCACACAGGACAGATTGG
  AGCACTGAAGCTGCTCACGAACTCCTCGGCCTTGTGGAGGTCCTCCGAAGCGCCTGAGACACTGCAGAGG
  GTATCAGGAATTTCTTTCCCCAAGGCAGAGTTACTGAGGAATTGGGAAGCTCGAAGGGAGGAAGCAGAGT
  TAAGAGATCACAGACGCATCGGGAAGGAACAGGAGCTCTTCTTCTTCCATGAGCTGAGCCCTGGGAGCTG
  CTTTTTCCTGCCACGAGGGACAAGAATTTATAACGCCCTGGTGGCTTTTATCAGGGCTGAGTATGCCCGA
  CGAGGTTTCTCAGAGGTGAAAACTCCCACGCTGTTTTCTACAAAACTCTGGGAACAGTCAGGGCACTGGG
  AGCATTATAGGGCACACATGTTTTCCCTAAAGCCCCCAGGCACTGATGGTGTCGACAGCTCCCAGAGTGG
  CCATCCTGCCAGGTGTCCCAAAGACACACTTGCTCTAAAGCCCATGAACTGCCCTGCACACTGCCTGATG
  TTTGCCCACCGGCCCAGATCCTGGCGAGAACTGCCTGTGCGGCTGGCTGATTTCGGAGTCCTGCATCGTG
  CGGAGGCCTCTGGCAGTCTGGGAGGATTGACGCGGCTGTGGCGCTTCCAGCAGGATGATGCTCACATCTT
  TTGTGCGCCCAGTCAGCTGGAAGCAGAGATCCGGGGCTGCCTTGATTTTCTCCGGTCTGTCTACTCGGTT
  CTTGGTTTCTCCTTCCACCTGGCTTTGTCTACCCGACCACCCGGCTTCCTAGGGGAGCCTCACCTATGGG
  ACCAGGCTGAGAAGGTCCTACAACAAGCCTTGGAAGAGTTTGGAGAACCCTGGAACCTCAACCCTGGAGA
  TGGGGCGTTCTATGGGCCTAAGATTGATGTGCACCTCCATGATGCCCTTGGCCGGCCCCATCAATGCGGA
  ACAATCCAGCTTGACTTCCAGCTGCCACTGAGATTTGACCTACAGTACAAGGGGCCGGCGGGGGCCCCCG
  AGTGTCCAGTCCTTATTCATCGGGCAGTGCTGGGGTCTGTGGAAAGGCTCTTGGGAGTGCTGGCAGAAAG
  CTGTGGGGGGAGATGGCCCCTGTGGCTGTCCCCGTTCCAGGTGGTGGTCATCCCTGTGAGGACAGAGCAA
  GAGGACTATGCCAGGCAGGTGCAACAGTGCCTGCAGGCCGCAGGGCTTGTGAGTGACCTCGATGCGGACT
  GTGGACTGACCCTCAGCCGGAGAGTCCGCCGGGCCCAGCTTGCCCACTACAATTTTCAGTTTGTGGTTGG
  CCAGAGAGAGCAGAGTCAAATGTCAGTGAACGTGCGCACTCGAGATAACCGGCAGCTGGGGGAGCGAGGC
  CTGGCTGAGTCAGTGCAGAGGCTGCTGGAGTTACAGGACGCCAGGGTCCCCAACGCAGAAGAACTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207239 representing NM_001014040
  Red=Cloning site Green=Tags(s)
  
  MGLCLRWRRLGFPLPGFRRCELHTVREAPIPTPPHWLAERFGLFEELWTAQVKRLASMTQKKARTIKISL
  PEGQKVDAVAWNTTPYQLAQQISSTLADTAVAAEVNGELYDLDRPLETDCHLRFLTFDSPEGKAVFWRSS
  AHVLGAAAEQHLGAVLCRGPSTESGFYLDFFLGKERTVRSTELPTLERICQEIITAAQPFRRLEASRGQL
  RQLFKDNHFKLHVIEEKVTGTTATVYGCGMSVDLCQGPHLRHTGQIGALKLLTNSSALWRSSEAPETLQR
  VSGISFPKAELLRNWEARREEAELRDHRRIGKEQELFFFHELSPGSCFFLPRGTRIYNALVAFIRAEYAR
  RGFSEVKTPTLFSTKLWEQSGHWEHYRAHMFSLKPPGTDGVDSSQSGHPARCPKDTLALKPMNCPAHCLM
  FAHRPRSWRELPVRLADFGVLHRAEASGSLGGLTRLWRFQQDDAHIFCAPSQLEAEIRGCLDFLRSVYSV
  LGFSFHLALSTRPPGFLGEPHLWDQAEKVLQQALEEFGEPWNLNPGDGAFYGPKIDVHLHDALGRPHQCG
  TIQLDFQLPLRFDLQYKGPAGAPECPVLIHRAVLGSVERLLGVLAESCGGRWPLWLSPFQVVVIPVRTEQ
  EDYARQVQQCLQAAGLVSDLDADCGLTLSRRVRRAQLAHYNFQFVVGQREQSQMSVNVRTRDNRQLGERG
  LAESVQRLLELQDARVPNAEELF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014040
• ORF Size: 2169 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014040.1, NP_001014062.1
• RefSeq Size: 2400 bp
• RefSeq ORF: 2172 bp
• Locus ID: 310672
• UniProt ID: Q68FW7
• Cytogenetics: 2q34
• MW: 81.7 kDa
• Gene Summary: Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged tRNA(Thr) via its editing domain.[UniProtKB/Swiss-Prot Function]