Nlrx1 (NM_001025010) Rat Tagged ORF Clone

CAT#: RR206718

Nlrx1 (Myc-DDK-tagged ORF) - Rat NLR family member X1 (Nlrx1), (10 ug)

Product Images

Circular map for RR206718

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nlrx1
• Synonyms: RGD1311293
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206718 representing NM_001025010
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTGGGGCTGCCATTTGCCCAGGACCTCTTGGGGCTCTGGCCTGGGAAGAACAGTCCCGCTCCCAG
  ACGAGCTCCGGATTCAGTCGAGCTGGCCCTTTAAAGGGGTGCATCATCCCCTTAGGCCCCCTAGGGCCTT
  TATCCGTCACCATGGAAACTCGGCAGACAGTGCTCCCCCACCAGGGAGGCATGGGCAGCTGTTCAGGAGC
  ATCTCTGCCACTGAAGCTATCCAAAGGCATCGTCGGAACCTCACTGAGTGGTTTAGCCGGCTGCCCAGAG
  AGGAGCGCCAGTTTGGACCAACCTTTGCTCTAGACACAGTTCACGTTGACCCCGTGATCCGAGAGAGCAC
  CCCAGATGACCTGCTTCGTCCATCCACGGAGCTGGCCACAGGGCATCAGCGAACCCAGGCAGAGCTCCCC
  CCACTGGCCCTGTCTCAGCTCTTTGACCCAGATGCCTGTGGGCGCCGCGTGCAGACAGTGGTATTGTATG
  GGACCGTGGGTACTGGCAAGAGCACATTGGTGCGCAAGATGGTCTTAGACTGGTGTTATGGGAGACTGCC
  TGCCTTTGAGCTGCTCATCCCCTTCTCCTGTGAGGACCTGTCATCCCTGGGCTCCACCCCAGCTTCCTTA
  TGCCAACTTGTGACCCAGCGTTACACACCCCTGAAAGAGGTGTTGCCCCTGATGAATGCTGCTGGATCCC
  GCCTGCTCTTTGTGCTCCATGGCTTGGAACGCCTCAACCTTGACTTCCGGCTGGCAGGCACAGGGCTTTG
  CAGTGACCCGGAGGAACCCGGGGCACCAGCTGCCATCATGGTCAACCTGCTGCGCAAATACATGCTTCCT
  GAGGCCAGCATTCTGGTAACCACCCGGCCCTCCGCCATTGGCCGTATCCCTAGCAAGTACGTGGGCCGCT
  ATGGTGAAATCTGTGGCTTCTCGGATACCAACCTGCAGAAGCTCTACTTCCAGCTCCGCCTTAACCAGCC
  TGACTGTGGGTATGGTGCTGGGGGTACAGGTGTCTCAGTTACACCAGCTCAGCGGGACAATCTCATTCAA
  ATGCTGTCCCGAAACCTGGAGGGGCACCACCAGATCGCAGCCGCCTGCTTTCTGCCCTCCTACTGCTGGC
  TTGTCTGTGCTACCTTGCACTTCCTGCATGCTCCCACATCTGCTGGTCAGACTCTCACAAGCATCTATAC
  CAGCTTTCTACGTCTGAACTTCAATGGGGAAACACTGGACAGCACCGACCCCTCCAACTTATCCCTGATG
  TCCTATGCAGCCCGGACTATGGGTAAGTTGGCCTATGAGGGGGTGTCATCCCGAAAGACCTACTTCTCTG
  AAGAGGATGTCCGTGGCTGCCTGGAAGCTGGCATCAAGACAGAGGAGGAGTTTCAGCTGCTCCAGATCTT
  CCGCCGGGATGCCCTGAGGTTTTTCCTGGCCCCGTGTGTGGAACCAGGGCACCTGGGTACCTTCGTATTC
  ACCGTACCTGCCATGCAGGAGTATCTGGCTGCCCTCTACATCGTGCTTGGTTTGCGCAAGACAGCCCTGC
  AGCGGGTGGGCAAAGAAGTGTTTGAATTTGTGGGCCGTGTTGGGGAAGATGTCAGCCTGGTATTGGGCAT
  TGTGGCCAAACTGTTGCCCCTGCGGATTCTGCCTCTGCTATTCAACTTGCTTAAGGTGGTTCCACGAGTG
  TTTGGGCGCATGGTGAGTAAGAGTCGCGAGGCGGTGGCTCAGGCCATGGTGCTGGAGATGTTCCGGGAGG
  AAGACTATTACAATGACGATGTTCTGGATCAGATGGGTGCCAGCATCCTGGGTGTGGAGGGCCCCCGGCG
  CCACCCAGATGAACCCCCTGAGGATGAAGTCTTTGAGCTCTTCCCCATGTTCATGAGTGGACTTCTCTCT
  GCCCACAATCGGGCGGTGTTGGCTCAGCTTGGCTGTCCCATTAAGAACCTGGATGCCCTGGAGAATGCCC
  AGGCAATCAAGAAGAAACTGGGGAAGATGGGTCGGCAGGTGCTGCCCCCCTCGGAGCTTCTTGACCATCT
  CTTCTTTCACTATGAGTTCCAGAACCAGCGCTTCTCAGCTGAGGTGCTGGGCTCCCTACGACAGCTCAAT
  TTAGCAGGTGTGCGAATGACACCCCTCAAGTGCACGGTGGTAGCGTCTGTACTGGGAAGTGGACGGCACC
  CCCTGGATGAAGTGAACTTGGCCTCCTGCCAGCTGGACCCCGCTGGGCTACACACTCTCATGCCTGTTCT
  CCTGCGGGCCCGGAAACTGGGGTTGCAACTCAACAATCTGGGCCCTGAGGCCTGCAGAGACCTCCGAGAC
  CTGCTGTTACATGACCAATGCCAGATCACCACTCTCCGGCTGTCCAACAACCCACTGACAGCAGCAGGGG
  TGGGTGTGCTGATGGACGGGCTGGCAGGAAACACTTCGGTGACACACCTGTCCCTGCTGCACACTGACCT
  TGGAGATGAGGGACTGGAGTTGCTGGCTGCCCAGCTGGACCGTAACAAACAACTACAGGAACTGAATGTG
  GCCTACAATGGTGCTGGGGACACGGTGGCTCTGGCCTTGGCTAAGGCTGCTCGGAAGCACCCGTCCCTGG
  AGCTGCTGCACCTCTACTTCAATGAGCTGAGTTCAGAGGGTCGCCAGGTCCTGCGGGATTTGGGCGGCTC
  TGGCGAAGGTGGTGCCCGGGTTGTAGCCTCGCTGACAGAGGGGACAGCGGTGTCTGAGTACTGGTCAGTG
  ATCCTCAGTGAAGTCCAACGCAACCTCAACAGCTGGGACCCTGTCCGGGTCCAGCGCCATCTCAAGCTGC
  TGCTCCGAGACTTGGAAGACAGCAGAGGTGCCACCCTTAATCCTTGGCGCAAGGCTCAGCTTCTGCGGGT
  GGAGAGCGAGGTCAAGACTCTTCTGGAGCAGCTGGGAGGCTCTGGACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206718 representing NM_001025010
  Red=Cloning site Green=Tags(s)
  
  MRWGCHLPRTSWGSGLGRTVPLPDELRIQSSWPFKGVHHPLRPPRAFIRHHGNSADSAPPPGRHGQLFRS
  ISATEAIQRHRRNLTEWFSRLPREERQFGPTFALDTVHVDPVIRESTPDDLLRPSTELATGHQRTQAELP
  PLALSQLFDPDACGRRVQTVVLYGTVGTGKSTLVRKMVLDWCYGRLPAFELLIPFSCEDLSSLGSTPASL
  CQLVTQRYTPLKEVLPLMNAAGSRLLFVLHGLERLNLDFRLAGTGLCSDPEEPGAPAAIMVNLLRKYMLP
  EASILVTTRPSAIGRIPSKYVGRYGEICGFSDTNLQKLYFQLRLNQPDCGYGAGGTGVSVTPAQRDNLIQ
  MLSRNLEGHHQIAAACFLPSYCWLVCATLHFLHAPTSAGQTLTSIYTSFLRLNFNGETLDSTDPSNLSLM
  SYAARTMGKLAYEGVSSRKTYFSEEDVRGCLEAGIKTEEEFQLLQIFRRDALRFFLAPCVEPGHLGTFVF
  TVPAMQEYLAALYIVLGLRKTALQRVGKEVFEFVGRVGEDVSLVLGIVAKLLPLRILPLLFNLLKVVPRV
  FGRMVSKSREAVAQAMVLEMFREEDYYNDDVLDQMGASILGVEGPRRHPDEPPEDEVFELFPMFMSGLLS
  AHNRAVLAQLGCPIKNLDALENAQAIKKKLGKMGRQVLPPSELLDHLFFHYEFQNQRFSAEVLGSLRQLN
  LAGVRMTPLKCTVVASVLGSGRHPLDEVNLASCQLDPAGLHTLMPVLLRARKLGLQLNNLGPEACRDLRD
  LLLHDQCQITTLRLSNNPLTAAGVGVLMDGLAGNTSVTHLSLLHTDLGDEGLELLAAQLDRNKQLQELNV
  AYNGAGDTVALALAKAARKHPSLELLHLYFNELSSEGRQVLRDLGGSGEGGARVVASLTEGTAVSEYWSV
  ILSEVQRNLNSWDPVRVQRHLKLLLRDLEDSRGATLNPWRKAQLLRVESEVKTLLEQLGGSGH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025010
• ORF Size: 2919 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025010.1, NP_001020181.1
• RefSeq Size: 3554 bp
• RefSeq ORF: 2922 bp
• Locus ID: 315599
• UniProt ID: Q5FVQ8
• Cytogenetics: 8q22
• MW: 107.6 kDa
• Gene Summary: Participates in antiviral signaling.[UniProtKB/Swiss-Prot Function]