Scn2a (NM_012647) Rat Tagged ORF Clone

CAT#: RR206256

Scn2a1 (Myc-DDK-tagged ORF) - Rat sodium channel, voltage-gated, type II, alpha 1 (Scn2a1), (10 ug)

Product Images

Circular map for RR206256

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Scn2a
• Synonyms: NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN; Scn2a1; Scn2a2; ScpII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206256 representing NM_012647
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACGGTCAGTGCTGGTACCTCCAGGACCTGACAGCTTCCGCTTCTTTACCAGGGAATCCCTTGCTG
  CTATCGAACAGCGCATTGCAGAAGAGAAAGCTAAGAGACCCAAACAGGAACGCAAGGACGAAGACGATGA
  AAATGGCCCAAAGCCAAATAGTGACTTGGAAGCAGGAAAATCCCTTCCTTTTATTTATGGAGACATTCCT
  CCAGAGATGGTGTCAGAACCACTGGAGGACCTGGACCCCTACTATATCAATAAGAAAACCTTTATAGTAT
  TGAATAAGGGGAAAGCAATCTCGCGGTTCAGCGCCACCTCTGCCCTGTACATTTTAACTCCCTTCAACCC
  CATTAGAAAATTAGCTATTAAGATTTTGGTACATTCTTTATTCAATGTGCTTATCATGTGCACAATTCTT
  ACCAACTGTGTGTTTATGACCATGAGTAACCCTCCAGACTGGACAAAGAATGTGGAGTATACTTTTACAG
  GAATTTATACTTTTGAATCACTTATTAAAATCCTTGCAAGGGGCTTTTGTCTAGAAGATTTCACATTTCT
  ACGGAATCCCTGGAATTGGTTGGATTTCACAGTCATTACTTTTGCGTATGTAACAGAATTTGTAAACCTA
  GGCAATGTTTCAGCTCTTCGAACTTTCAGAGTCTTGAGAGCTTTGAAAACTATTTCTGTAATTCCAGGCC
  TGAAGACTATCGTGGGGGCCCTGATCCAGTCAGTGAAGAAGCTCTCTGACGTCATGATCCTCACCGTGTT
  CTGTCTCAGTGTCTTTGCTCTAATCGGGCTGCAGCTCTTCATGGGCAACCTGAGGAATAAATGCTTGCAG
  TGGCCCCCAGACAATTCTACCTTTGAAATAAATATCACTTCCTTCTTTAATAACTCATTGGATTGGAATG
  GTACTGCCTTCAATAGGACGGTGAACATGTTTAACTGGGACGAATATATTGAAGATAAAAGTCACTTTTA
  TTTTTTGGAAGGACAAAACGATGCTCTGCTTTGTGGGAACAGCTCGGATGCTGGACAGTGTCCAGAAGGA
  TACATCTGTGTGAAGGCTGGGAGAAACCCCAACTACGGCTACACAAGTTTTGACACCTTCAGCTGGGCCT
  TCTTGTCCTTATTTCGTCTCATGACTCAAGACTTCTGGGAAAACCTTTATCAGCTGACCTTGCGTGCTGC
  CGGGAAAACATACATGATATTTTTTGTGCTGGTCATTTTCTTGGGCTCATTCTACCTGATAAATTTGATC
  CTGGCTGTGGTGGCCATGGCCTACGAGGAACAGAACCAGGCCACACTGGAGGAGGCTGAACAGAAGGAGG
  CAGAGTTTCAGCAGATGCTGGAGCAACTGAAGAAGCAGCAGGAGGAAGCTCAGGCAGCAGCTGCAGCAGC
  GTCCGCAGAATCCAGAGACTTCAGCGGGGCAGGCGGGATAGGTGTTTTCTCGGAGAGTTCGTCAGTAGCC
  TCAAAGTTAAGTTCCAAAAGTGAAAAGGAGTTGAAAAACAGAAGAAAGAAAAAGAAACAGAAAGAACAGG
  CTGGGGAGGAAGAGAAGGAAGACGCGGTGCGGAAATCTGCCTCTGAGGACAGCATACGAAAGAAAGGCTT
  CCAGTTTTCCCTGGAAGGAAGTAGATTGACCTACGAGAAGAGATTTTCCTCTCCGCACCAGTCTCTCTTG
  AGCATCCGAGGCTCCCTATTTTCTCCAAGACGCAACAGTAGAGCAAGCCTTTTCAACTTCAAAGGTCGAG
  TGAAGGATATTGGTTCCGAAAATGACTTTGCAGACGATGAACACAGCACATTTGAGGACAACGACAGCAG
  AAGAGACTCTCTATTTGTACCACACAGACATGGAGAAAGGCGTCCTAGCAATGTCAGCCAGGCCAGCCGT
  GCCTCCCGGGGGATACCCACTCTACCCATGAATGGGAAGATGCACAGTGCAGTGGACTGCAACGGTGTGG
  TGTCCCTGGTTGGAGGCCCCTCTGCGCTCACATCGCCTGTGGGGCAGCTGCTGCCGGAGGGCACAACTAC
  TGAGACAGAAATAAGGAAGAGGAGATCCAGTTCTTACCACGTCTCTATGGACTTGTTGGAAGACCCTTCA
  AGGCAAAGGGCAATGAGTATGGCCAGTATTTTGACGAACACTATGGAAGAACTTGAAGAATCCAGACAGA
  AATGCCCACCCTGCTGGTATAAATTTGCTAATATGTGCCTGATTTGGGACTGTTGTAAGCCATGGCTAAA
  GGTAAAACACGTTGTCAATCTGGTAGTGATGGATCCATTTGTTGACCTGGCCATCACCATCTGCATTGTG
  TTAAATACGCTCTTCATGGCCATGGAGCACTACCCCATGACTGAGCAGTTCAGCAGTGTGCTGTCTGTTG
  GGAATTTGGTCTTCACTGGGATCTTCACGGCAGAAATGTTTCTCAAGATAATAGCCATGGATCCATATTA
  TTACTTCCAAGAAGGCTGGAATATCTTTGATGGCTTTATAGTGAGCCTTAGTTTAATGGAACTCGGCTTG
  GCGAATGTGGAAGGATTGTCAGTTCTCCGATCATTCCGGCTGCTTAGAGTCTTCAAGTTGGCAAAGTCCT
  GGCCCACACTGAACATGCTCATTAAGATCATCGGCAACTCGGTGGGTGCACTGGGCAACCTGACCCTGGT
  GCTGGCCATCATCGTCTTCATTTTTGCCGTGGTCGGCATGCAGCTGTTTGGAAAGAGCTACAAGGAGTGT
  GTCTGTAAGATTTCCAATGATTGTGAGCTCCCGCGCTGGCACATGCACCACTTCTTCCACTCCTTCCTGA
  TCGTGTTTCGAGTCCTGTGTGGGGAGTGGATAGAGACCATGTGGGACTGCATGGAGGTCGCGGGCCAGAC
  CATGTGCCTTACTGTCTTCATGATGGTCATGGTGATTGGGAACCTTGTTGTCCTGAACCTCTTCTTGGCC
  TTGCTCCTCAGTTCTTTCAGCTCAGACAACCTGGCTGCCACAGATGACGATAACGAAATGAACAACCTCC
  AGATAGCCGTGGGAAGGATGCAGAAGGGAATTGATTTTGTTAAAAGGAAGATACGTGAATTCATTCAGAA
  AGCCTTTGTCAGAAAGCAGAAAGCTTTAGATGAAATCAAACCGCTGGAAGATCTGAATAACAAGAAAGAC
  AGTTGTATCTCCAACCACACGACCATAGAAATAGGCAAGGACCTCAATTACCTCAAAGATGGAAACGGGA
  CGACCAGTGGCATAGGCAGCAGTGTGGAGAAGTATGTGGTAGATGAGAGTGATTACATGTCGTTCATAAA
  CAACCCCAGCCTCACCGTGACTGTGCCCATCGCCCTTGGAGAGTCTGACTTTGAAAACTTAAACACGGAA
  GAATTCAGTAGTGAGTCAGATATGGAAGAAAGCAAGGAGAAATTGAATGCAACTAGCTCATCTGAGGGCA
  GTACGGTTGATATAGGCGCTCCGGCAGAGGGAGAGCAGCCAGAGGCCGAACCGGAAGAATCGCTTGAACC
  GGAAGCGTGTTTCACAGAAGACTGTGTGAGAAAGTTCAAGTGTTGTCAGATAAGCATAGAAGAGGGCAAA
  GGCAAGCTCTGGTGGAACTTGAGGAAAACGTGCTACAAGATAGTAGAGCATAACTGGTTTGAAACCTTCA
  TCGTTTTTATGATTCTGCTCAGCAGTGGTGCTCTGGCCTTTGAAGACATTTATATTGAGCAGCGAAAGAC
  CATCAAGACCATGCTGGAGTATGCTGACAAGGTTTTCACTTATATCTTCATCCTGGAGATGCTTCTAAAG
  TGGGTGGCCTATGGTTTCCAAATGTATTTCACCAATGCCTGGTGCTGGCTGGACTTCCTGATCGTTGATG
  TCTCCTTGGTTAGCTTAACTGCAAATGCCTTGGGCTATTCGGAACTTGGTGCCATCAAGTCCCTCCGAAC
  GTTAAGAGCTCTGAGACCTCTACGAGCCTTATCCCGATTTGAAGGAATGAGGGTTGTTGTAAACGCTCTC
  TTAGGCGCCATCCCCTCCATAATGAACGTACTTCTGGTCTGCCTGATCTTTTGGCTAATATTCAGTATCA
  TGGGGGTGAATCTCTTTGCTGGCAAGTTCTACCATTGCATCAACTACACCACGGGGGAAATGTTTGATGT
  GAGCGTGGTCAACAACTACAGTGAATGCCAAGCTCTCATTGAGAGCAATCAGACGGCCAGGTGGAAGAAC
  GTGAAGGTCAACTTCGATAACGTGGGACTCGGATACCTTTCTCTGCTCCAAGTAGCCACCTTTAAAGGAT
  GGATGGATATCATGTATGCAGCTGTTGACTCAAGAAATGTAGAACTGCAGCCCAAATACGAAGACAATCT
  CTACATGTACCTTTACTTTGTCATCTTCATCATCTTCGGCTCATTCTTCACCCTGAACCTGTTCATTGGT
  GTCATCATAGACAACTTCAACCAGCAGAAGAAGAAGTTTGGAGGTCAAGACATCTTTATGACAGAAGAAC
  AGAAGAAATACTACAATGCAATGAAGAAGCTCGGCTCCAAGAAACCGCAGAAGCCCATTCCTCGGCCTGC
  AAACAAATTTCAAGGGATGGTCTTTGATTTTGTAACCAAACAAGTCTTTGACATCAGCATCATGATCCTC
  ATCTGCCTCAACATGGTGACCATGATGGTGGAAACGGACGACCAGAGTCAGGAGATGACCAACATCCTGT
  ACTGGATCAACCTGGTGTTCATTGTCCTGTTCACGGGCGAGTGTGTGCTGAAGCTCATCTCCCTCCGCCA
  TTATTATTTCACCATCGGCTGGAATATTTTTGATTTTGTGGTAGTCATCCTCTCCATTGTAGGAATGTTT
  CTCGCGGAGCTGATAGAGAAGTATTTCGTGTCCCCTACCCTGTTCCGAGTCATCCGCCTGGCCAGGATTG
  GACGAATCCTACGCCTGATCAAAGGCGCCAAGGGGATCCGCACTCTGCTCTTTGCTCTGATGATGTCCCT
  TCCTGCGCTGTTCAACATCGGCCTCCTGCTTTTCCTGGTCATGTTCATCTACGCCATCTTTGGGATGTCC
  AACTTTGCCTACGTTAAGAGGGAAGTTGGAATTGATGACATGTTCAACTTTGAGACTTTTGGCAACAGCA
  TGATCTGCCTGTTCCAAATCACCACCTCTGCCGGCTGGGACGGACTGCTGGCCCCTATACTAAATAGCGG
  ACCTCCTGACTGTGACCCTGAAAAAGATCACCCTGGAAGCTCGGTGAAGGGGGACTGTGGGAACCCATCT
  GTGGGGATTTTCTTTTTTGTCAGCTACATCATCATATCCTTCCTGGTGGTGGTGAACATGTACATCGCTG
  TCATCCTGGAGAACTTCAGCGTCGCCACCGAAGAAAGTGCAGAGCCTCTGAGTGAGGACGACTTTGAGAT
  GTTCTACGAGGTCTGGGAGAAGTTCGACCCTGACGCCACTCAGTTCATAGAGTTCTGCAAGCTTTCTGAC
  TTTGCAGCTGCCCTGGATCCTCCCCTCCTCATCGCAAAGCCAAACAAAGTCCAGCTCATTGCCATGGACC
  TGCCCATGGTGAGTGGAGACCGCATCCACTGCCTGGACATCTTGTTTGCTTTTACAAAGCGGGTCCTGGG
  CGAGAGTGGAGAGATGGACGCTCTTCGAATCCAGATGGAAGAAAGGTTCATGGCTTCCAACCCCTCCAAG
  GTCTCTTATGAGCCCATTACCACCACCCTGAAACGGAAACAAGAGGAGGTGTCTGCTATTGTCATCCAGC
  GAGCATACAGGCGGTATCTCCTGAAGCAGAAAGTTAAGAAAGTTTCGTCTATCTATAAAAAAGACAAAGG
  TAAAGAAGACGAGGGAACGCCCATCAAAGAAGACATCATCACAGATAAACTGAACGAGAATTCAACTCCA
  GAGAAGACTGATGTGACACCTTCCACAACTTCTCCACCTTCCTACGATAGCGTGACGAAACCGGAGAAGG
  AAAAATTTGAAAAGGACAAATCAGAAAAAGAGGACAAAGGGAAAGATATCAGGGAAAGTAAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206256 representing NM_012647
  Red=Cloning site Green=Tags(s)
  
  MARSVLVPPGPDSFRFFTRESLAAIEQRIAEEKAKRPKQERKDEDDENGPKPNSDLEAGKSLPFIYGDIP
  PEMVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATSALYILTPFNPIRKLAIKILVHSLFNVLIMCTIL
  TNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRNPWNWLDFTVITFAYVTEFVNL
  GNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQ
  WPPDNSTFEINITSFFNNSLDWNGTAFNRTVNMFNWDEYIEDKSHFYFLEGQNDALLCGNSSDAGQCPEG
  YICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLI
  LAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEEAQAAAAAASAESRDFSGAGGIGVFSESSSVA
  SKLSSKSEKELKNRRKKKKQKEQAGEEEKEDAVRKSASEDSIRKKGFQFSLEGSRLTYEKRFSSPHQSLL
  SIRGSLFSPRRNSRASLFNFKGRVKDIGSENDFADDEHSTFEDNDSRRDSLFVPHRHGERRPSNVSQASR
  ASRGIPTLPMNGKMHSAVDCNGVVSLVGGPSALTSPVGQLLPEGTTTETEIRKRRSSSYHVSMDLLEDPS
  RQRAMSMASILTNTMEELEESRQKCPPCWYKFANMCLIWDCCKPWLKVKHVVNLVVMDPFVDLAITICIV
  LNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVSLSLMELGL
  ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKEC
  VCKISNDCELPRWHMHHFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLA
  LLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDFVKRKIREFIQKAFVRKQKALDEIKPLEDLNNKKD
  SCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVVDESDYMSFINNPSLTVTVPIALGESDFENLNTE
  EFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEAEPEESLEPEACFTEDCVRKFKCCQISIEEGK
  GKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLK
  WVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNAL
  LGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECQALIESNQTARWKN
  VKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIG
  VIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMIL
  ICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMF
  LAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMS
  NFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPS
  VGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFCKLSD
  FAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSK
  VSYEPITTTLKRKQEEVSAIVIQRAYRRYLLKQKVKKVSSIYKKDKGKEDEGTPIKEDIITDKLNENSTP
  EKTDVTPSTTSPPSYDSVTKPEKEKFEKDKSEKEDKGKDIRESKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012647
• ORF Size: 6015 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012647.1, NP_036779.1
• RefSeq Size: 8553 bp
• RefSeq ORF: 6018 bp
• Locus ID: 24766
• UniProt ID: P04775
• Cytogenetics: 3q21
• MW: 227.9 kDa
• Gene Summary: Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with four repeat domains, each of which is composed of six membrane-spanning segments, and one or more regulatory beta subunits. Voltage-gated sodium channels are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family. In humans, variants of this gene are associated with seizure disorders and autism spectrum disorder. Mice homozygous for a knockout mutation die with severe hypoxia and extensive neuronal cell death, while gain of function mutations result in progressive seizure disorder. [provided by RefSeq, Nov 2016]