Prf1 (NM_017330) Rat Tagged ORF Clone

CAT#: RR205716

Prf1 (Myc-DDK-tagged ORF) - Rat perforin 1 (pore forming protein) (Prf1), (10 ug)

Product Images

Circular map for RR205716

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prf1
• Synonyms: Cyta; RATCYTA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205716 representing NM_017330
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGTACCTGTTCCTCCTGGGCCTTTTCCTGCTGCTGCCACGACCTGTCCCTGCTCCCTGCTACA
  CTGCCACTCGGTCAGAATGCAAGCAGAAGCACAAATTCGTGCCTGGTGTGTGGGCGGCTGGGGAAGGTGT
  GGATGTGACCACCCTCCGCCGCTCCAGCTCCTTCCCAGTGAACACAGGGAAGTTCCTGAGGCCTGACCGC
  ACCTGCACCCTCTGTAAAAACGCCCTGATGAATGACGCTATACAGCGCCTACCCGTGGCAATCGCCCACT
  GGCGGCCTCACGGCTCACACTGCCAGCGTAATGTGGCCACAACCAAGGTCAGCTCCACGGAGGGTGTGGC
  GCGGGAGGCGGCTGCTAATATCAATAACGACTGGCGTGCGGGGCTGGATGTGAACCCTAAACCGGAAGCA
  AACGTGCATGTGTCCGTGGCTGGCTCCCATTCCAAGATAGCCAATTTTGCAGCCGAGAAGGCCCATCAGG
  ACCAATACAACTTTAATACTGACACAGTGGAGTGTCGCATGTACAGTTTTCGCCTGGCACAAAAGCCTCC
  ACTCCACCCTGACTTCAGAAAGGCACTCAAGAACCTTCCCCACAACTTTAACAGCTCCACAGAGCATGCT
  TACCGTAGACTCATCTCTTCCTATGGGACTCATTTTATTACGGCTGTGGACCTAGGTGGCCGCGTCTCGG
  TCCTCACGGCTCTGCGTACCTGTCAGCTGACCCTGGATGGGCTCACAGCTGATGAGGTAGGAGACTGCTT
  GAGCGTGGAAGCCCAGGTCAGCATCGGTGCCCAAGCCAGTGTCTCAAGCGAATACAAAGCTTGCGAGGAG
  AAGAAGAAACAACACAAAATCGCCACCTCTTTCCACCAGACCTACCGTGAGCGTCATGTCGAAGTGCTCG
  GTGGCCCCCTTGACTCCTCGAATGACCTGCTCTTCGGGAACCAAGCTACCCCTGAGCACTTCTCTACCTG
  GATAGCCTCATTGCCCACCAGGCCTGATGTGGTGGACTACAGCCTGGAGCCCCTGCACATACTACTGGAA
  GACTCGGACCCGAAGCGAGAGGCACTGAGACAGGCTATCAGCCATTATGTGATGAGCAGGGCTCGCTGGC
  GAGACTGTAACCGGCCCTGCAGGGCAGGCCAGCATAAGAGTAGTCGTGATTCATGCCAGTGTGTGTGCCA
  GGATTCGAAGGTCACCAACCAGGACTGCTGCCCACGCCAGAGGGGCTTGGCCAAGTTGATGGTAAGGAAT
  TTCCAGGCCAAGGGTCTGTGGGGGGACTACATCACATCTACGGATGCTTATCTGAAGGTCTTCTTTGGTG
  GCCAGGAGATCAGGACCGGTGTAGTGTGGAACAATAACCATCCCTCGTGGAGTGACAAGATGGACTTTGG
  GAATGTGCTCCTGTCCACAGGGGGACCCCTCAGGGTGCAGGTCTGGGATGCTGACAATGGCTGGGATGAT
  GACCTTCTTGGTACTTGTGACAAGTCTCCCAAGTCTGGTTTCCATGAGGTGAATTGTCCCCTGAACCACG
  GCAGTATAAAATTCATCTACCAGGCCAATTGCTTGCCCCATCTCACTGGAGAGACCTGCCTGGAGTATGC
  CCCCCAGGGGCTTCTAGGAGATCCACCAGGAAACCGCAGTGGGGCTGTGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205716 representing NM_017330
  Red=Cloning site Green=Tags(s)
  
  MAAYLFLLGLFLLLPRPVPAPCYTATRSECKQKHKFVPGVWAAGEGVDVTTLRRSSSFPVNTGKFLRPDR
  TCTLCKNALMNDAIQRLPVAIAHWRPHGSHCQRNVATTKVSSTEGVAREAAANINNDWRAGLDVNPKPEA
  NVHVSVAGSHSKIANFAAEKAHQDQYNFNTDTVECRMYSFRLAQKPPLHPDFRKALKNLPHNFNSSTEHA
  YRRLISSYGTHFITAVDLGGRVSVLTALRTCQLTLDGLTADEVGDCLSVEAQVSIGAQASVSSEYKACEE
  KKKQHKIATSFHQTYRERHVEVLGGPLDSSNDLLFGNQATPEHFSTWIASLPTRPDVVDYSLEPLHILLE
  DSDPKREALRQAISHYVMSRARWRDCNRPCRAGQHKSSRDSCQCVCQDSKVTNQDCCPRQRGLAKLMVRN
  FQAKGLWGDYITSTDAYLKVFFGGQEIRTGVVWNNNHPSWSDKMDFGNVLLSTGGPLRVQVWDADNGWDD
  DLLGTCDKSPKSGFHEVNCPLNHGSIKFIYQANCLPHLTGETCLEYAPQGLLGDPPGNRSGAVW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017330
• ORF Size: 1662 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017330.2, NP_059026.2
• RefSeq Size: 1964 bp
• RefSeq ORF: 1665 bp
• Locus ID: 50669
• Cytogenetics: 20q11
• MW: 61.5 kDa
• Gene Summary: cytolytic factor present in cytoplasmic granules [RGD, Feb 2006]