Abcb4 (NM_012690) Rat Tagged ORF Clone

CAT#: RR205358

Abcb4 (Myc-DDK-tagged ORF) - Rat ATP-binding cassette, sub-family B (MDR/TAP), member 4 (Abcb4), (10 ug)

Product Images

Circular map for RR205358

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcb4
• Synonyms: Mdr2; Pgy3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205358 representing NM_012690
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTTGAGGCAGCAAGAAACGGAACAGCGCGGCGCCTGGACGGCGACTTTGAACTAGGCAGCATCA
  GCAACCAGAGCAGAGAAAAAAAGAAGAAAGTGAATTTAATTGGCCCGTTGACACTGTTCCGATACTCTGA
  TTGGCAGGATAAATTGTTTATGCTCCTGGGCACCGCCATGGCCATAGCTCACGGATCAGGTCTTCCCCTT
  ATGATGATAGTCTTTGGAGAAATGACAGATAAGTTTGTAGATAATGCTGGGAACTTTTCCTTGCCAGTGA
  ATTTTTCATTGTCAATGCTAAATCCAGGAAGAATTCTGGAAGAAGAAATGACTAGATATGCATACTACTA
  TTCGGGACTAGGTGGTGGAGTTCTTTTGGCTGCCTATATCCAAGTCTCCTTCTGGACTTTGGCAGCTGGC
  CGACAAATAAGGAAAATCAGGCAAAAATTTTTTCACGCCATCCTTCGACAAGAAATGGGCTGGTTTGATA
  TCAAGGGCACCACCGAACTCAACACGCGGCTGACAGATGACATCTCCAAAATCAGTGAAGGAATTGGTGA
  CAAGGTTGGAATGTTCTTTCAAGCAATAGCCACGTTTTTTGCAGGATTCATAGTGGGGTTCATCAGAGGC
  TGGAAACTCACCCTCGTGATCATGGCCATCACCGCCATCTTGGGGCTCTCTACAGCCGTTTGGGCAAAGA
  TACTCTCAACATTCAGTGACAAAGAACTAGCTGCCTATGCAAAAGCAGGTGCCGTGGCGGAAGAGGCTCT
  GGGAGCCATCAGGACCGTGATAGCTTTCGGGGGCCAGAACAAAGAGCTAGAAAGGTATCAGAAGCATTTA
  GAAAATGCCAAAAAGATTGGAATTAAAAAGGCTATCTCGGCCAACATCTCCATGGGCATTGCCTTTTTGT
  TAATATATGCATCCTATGCACTGGCCTTCTGGTATGGATCCACTCTGGTTATATCAAAAGAATATACAAT
  TGGAAATGCCATGACAGTGTTCTTCTCAATCCTCATTGGGGCCTTCAGTGTGGGGCAGGCTGCCCCCTGT
  ATTGATGCTTTCCCCAATGCTAGAGGAGCAGCCTATGTGATCTTTGACATTATTGATAATAATCCTAAAA
  TTGACAGTTTTTCAGAGAGAGGACACAAGCCAGACAGCATCAAAGGAAATTTGGAGTTCAGTGACGTTCA
  CTTTTCCTACCCATCTCGGGCTAATATCAAGATCTTGAAGGGCCTCAACCTGAAGGTGAAGAGCGGGCAG
  ACGGTAGCCCTGGTTGGCAACAGTGGCTGTGGGAAAAGCACAACTGTCCAGCTGCTGCAGAGGCTCTACG
  ACCCCACAGAGGGTACGATTAGCATCGATGGGCAGGACATCCGGAACTTTAACGTCAGGTGTCTAAGGGA
  ATTCATCGGCGTGGTGAGTCAAGAGCCGGTACTGTTCTCTACCACGATTGCTGAAAATATCCGCTATGGC
  CGTGGGAATGTAACAATGGATGAGATTAAAAAAGCTGTCAAAGAGGCTAATGCCTATGACTTCATCATGA
  AACTGCCACAGAAATTTGACACCCTGGTTGGTGACAGAGGGGCGCAGCTGAGCGGGGGACAGAAACAGAG
  GATCGCCATTGCTCGTGCCTTGGTCCGCAACCCCAAGATCCTCCTGCTGGACGAGGCCACGTCAGCCTTG
  GACACAGAAAGCGAAGCTGAGGTGCAGGCCGCTCTGGATAAGGCCAGAGAAGGCCGGACCACCATCGTGA
  TAGCTCACCGACTGTCAACTGTCCGGAATGCAGATGTCATCGCTGGGTTTGAGGATGGCGTCATCGTGGA
  GCAAGGAAGCCACAGTGAGCTGATAAAGAAGGAAGGGATCTACTTCAGACTTGTTAACATGCAGACATCA
  GGAAGCCAGATCCTGTCAGAAGAATTTGAAGTTGAGCTAAGTGATGAAAAGGCTGCTGGAGGTGTGGCCC
  CAAATGGCTGGAAAGCACGCATATTTAGGAATTCTACGAAGAAAAGTCTGAAAAGTTCACGGGCGCATCA
  AAATAGGCTGGATGTGGAAACCAATGAACTTGATGCAAACGTGCCACCAGTGTCTTTTCTGAAGGTCTTA
  AGACTGAATAAAACAGAGTGGCCCTACTTTGTGGTGGGGACACTCTGTGCCATTGCCAACGGGGCCCTCC
  AGCCGGCATTCTCCATCATCCTGTCAGAGATGATAGCTATCTTTGGCCCTGGGGATGACACAGTAAAGCA
  ACAGAAGTGTAACATGTTCTCGCTGGTCTTCTTGGGCCTAGGAGTCCACTCCTTCTTTACTTTCTTCCTT
  CAGGGTTTCACATTCGGGAAAGCTGGCGAGATCCTCACCACAAGGCTCCGGTCCATGGCCTTCAAAGCAA
  TGCTAAGACAGGACATGAGCTGGTTTGACGATCATAAAAACAGTACTGGTGCCCTCTCTACAAGACTCGC
  CACAGACGCTGCGCAGGTCCAAGGAGCCACAGGAACCAGGTTGGCTTTAATTGCACAGAACACAGCCAAC
  CTTGGAACGGGTATTATTATATCATTTATTTACGGTTGGCAACTGACACTTCTGCTCTTATCAGTTGTTC
  CATTCATTGCTGTAGCGGGAATTGTTGAAATGAAAATGTTGGCTGGCAACGCCAAGAGAGATAAAAAGGA
  GATGGAAGCTGCTGGAAAGATTGCAACAGAGGCAATAGAAAATATTCGGACTGTTGTATCCTTGACCCAA
  GAGAGAAAATTTGAGTCAATGTATGTTGAAAAATTACACGGACCTTACAGGAATTCAGTGCGGAAGGCTC
  ACATCTACGGCATCACTTTTAGCATCTCACAAGCATTCATGTACTTTTCTTATGCTGGCTGCTTTCGATT
  TGGTTCTTACCTCATTGTGAATGGACACATGCGCTTCAAGGATGTCATCCTGGTGTTCTCAGCAATCGTG
  CTTGGTGCAGTGGCTCTAGGACATGCCAGCTCATTTGCTCCAGACTATGCAAAAGCCAAGCTGTCTGCAG
  CATACTTATTCAGTCTGTTTGAAAGACAACCTCTGATTGACAGCTACAGCAGAGAAGGAATGTGGCCGGA
  TAAGTTTGAAGGAAGCGTGACATTCAATGAAGTTGTGTTCAATTATCCCACCCGGGCCAATGTGCCAGTG
  CTTCAGGGGCTGAGCCTCGAGGTGAAGAAGGGGCAGACCCTGGCCCTGGTGGGCAGTAGTGGCTGCGGGA
  AGAGCACCGTGGTCCAGCTGCTCGAGCGCTTCTACGACCCCATGGCCGGAACAGTGCTCCTCGATGGTCA
  GGAAGCAAAGAAACTCAATGTCCAGTGGCTCCGAGCTCAACTTGGCATTGTGTCCCAGGAGCCCATCCTG
  TTTGACTGCAGCATCGCCAAGAACATCGCCTACGGAGACAACAGCCGTGTCGTGTCTCAGGATGAGATTG
  TGAGGGCGGCCAAGGAGGCCAACATCCACCCCTTCATTGAGACACTGCCCCAAAAGTATGAAACAAGAGT
  AGGAGACAAGGGGACACAGCTCTCTGGAGGCCAGAAACAGAGGATTGCTATCGCCCGAGCCCTCATCAGA
  CAGCCTCGGGTCCTACTGCTGGATGAAGCCACGTCGGCTTTGGACACTGAGAGTGAAAAGGTCGTCCAGG
  AAGCGCTGGACAAAGCCAGGGAAGGCCGCACCTGCATTGTGATCGCGCACCGCCTGTCCACCATCCAGAA
  CGCAGACTTGATCGTGGTGATCGACAACGGCAAGGTCAAGGAGCACGGCACCCACCAGCAGCTGCTGGCC
  CAGAAAGGCATCTATTTCTCCATGGTCAACATTCAAGCTGGCACACAGAACTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205358 representing NM_012690
  Red=Cloning site Green=Tags(s)
  
  MDLEAARNGTARRLDGDFELGSISNQSREKKKKVNLIGPLTLFRYSDWQDKLFMLLGTAMAIAHGSGLPL
  MMIVFGEMTDKFVDNAGNFSLPVNFSLSMLNPGRILEEEMTRYAYYYSGLGGGVLLAAYIQVSFWTLAAG
  RQIRKIRQKFFHAILRQEMGWFDIKGTTELNTRLTDDISKISEGIGDKVGMFFQAIATFFAGFIVGFIRG
  WKLTLVIMAITAILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHL
  ENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPC
  IDAFPNARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQ
  TVALVGNSGCGKSTTVQLLQRLYDPTEGTISIDGQDIRNFNVRCLREFIGVVSQEPVLFSTTIAENIRYG
  RGNVTMDEIKKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSAL
  DTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELIKKEGIYFRLVNMQTS
  GSQILSEEFEVELSDEKAAGGVAPNGWKARIFRNSTKKSLKSSRAHQNRLDVETNELDANVPPVSFLKVL
  RLNKTEWPYFVVGTLCAIANGALQPAFSIILSEMIAIFGPGDDTVKQQKCNMFSLVFLGLGVHSFFTFFL
  QGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTAN
  LGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRDKKEMEAAGKIATEAIENIRTVVSLTQ
  ERKFESMYVEKLHGPYRNSVRKAHIYGITFSISQAFMYFSYAGCFRFGSYLIVNGHMRFKDVILVFSAIV
  LGAVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSREGMWPDKFEGSVTFNEVVFNYPTRANVPV
  LQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPIL
  FDCSIAKNIAYGDNSRVVSQDEIVRAAKEANIHPFIETLPQKYETRVGDKGTQLSGGQKQRIAIARALIR
  QPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIDNGKVKEHGTHQQLLA
  QKGIYFSMVNIQAGTQNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012690
• ORF Size: 3834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012690.2, NP_036822.1
• RefSeq Size: 3910 bp
• RefSeq ORF: 3837 bp
• Locus ID: 24891
• UniProt ID: Q08201
• Cytogenetics: 4q12
• MW: 140.7 kDa
• Gene Summary: energy-dependent efflux pump; may be involved in changes in bile formation in response to diabetes [RGD, Feb 2006]