Tap1 (NM_032055) Rat Tagged ORF Clone

CAT#: RR204474

Tap1 (Myc-DDK-tagged ORF) - Rat transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) (Tap1), (10 ug)

Product Images

Circular map for RR204474

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tap1
• Synonyms: Abcb2; Cim; Tap2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204474 representing NM_032055
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGCACGCCTGGCCGACGGCCGCCTTGCTGCTGCTGCTGGTGGACTGGCTGCTGCTGCGGCCCG
  TGCTCCCGGGAATCTTCTCCCTGTTGGTTCCCGAGGTGCCGCTGCTCCGGGTCTGGGCCGTGGGCTTGAG
  TCGCTGGGCTATCCTGGGACTAGGGGTCCGCGGGGTCCTCGGGGTCACCGCGGGAGCCCGTGGCTGGCTG
  GCTGCTTTGCAGCCGCTGGTGGCGGCGCTGGGTTTGGCCCTGCCTGGACTTGCCTCGTTCCGAAAGCTGT
  CCGCCTGGGGAGCACTCCGGGAGGGTGACAACGCTGGACTGCTCCACTGGAACAGTCGCTTAGATGCCTT
  CGTTCTCAGTTATGTGGCCGCATTGCCCGCAGCTGCCCTGTGGCACAAGTTGGGGGGCTTCTGGGCGCCC
  AGTGGCCACAAGGGCGCTGGAGACATGCTGTGTCGGATGCTAGGCTTCCTGGACTCCAAGAAGGGGCGTC
  TCCACCTGGTTCTGGTTCTCTTGATCCTCTCCTGCCTTGGGGAAATGGCCATTCCCTTCTTCACAGGCCG
  CATCACTGACTGGATCCTTCAGGATAAGACAGCCCCCAGCTTCGCCCGCAACATGTGGCTCATGTGTATT
  CTTACCGTAGCCAGTACAGCGCTGGAGTTTGCAGGAGATGGAATCTACAACATCACCATGGGCCACATGC
  ACAGCCGCGTGCATGGAGAGGTGTTTCGGGCCGTCCTTCACCAGGAGACAGGATTTTTCCTGAAGAACCC
  AACAGGTTCCATCACATCTCGGGTGACTGAGGACACCTCCAACGTGTGCGAGTCCATTAGTGACAAGCTG
  AACCTGTTCCTGTGGTACCTGGGGCGAGGCCTGTGTCTCCTGGCGTTCATGATTTGGGGGTCATTCTACC
  TCACTGTGGTCACCCTGCTCAGCCTGCCTCTGCTTTTCCTTCTGCCCAGGAGGCTGGGGAAAGTGTACCA
  GTCACTGGCAGTGAAGGTGCAGGAGTCTCTAGCAAAGTCCACGCAGGTGGCCCTCGAGGCCCTGTCGGCG
  ATGCCTACCGTACGGAGCTTTGCCAACGAGGAGGGAGAGGCCCAGAAGTTTAGGCAGAAGTTGGAAGAAA
  TGAAGCCGCTAAACAAGAAAGAGGCCCTGGCTTACGTCACCGAAGTCTGGACCATGAGTGTCTCGGGAAT
  GCTGCTGAAGGTGGGAATTCTGTACCTCGGTGGGCAGCTGGTGGTCAGAGGGGCTGTCAGCAGCGGCAAC
  CTCGTCTCCTTTGTTCTCTACCAGCTTCAGTTCACCAGGGCCGTGGAGGTCCTGCTCTCCATCTATCCCT
  CCATGCAGAAGTCCGTGGGCGCCTCGGAGAAAATATTCGAATACCTGGACCGGACTCCCTGCTCTCCGCT
  CAGTGGCTCACTGGCACCTTTAAACATGAAAGGCCTCGTCAAGTTCCAAGATGTCTCCTTTGCCTACCCA
  AACCATCCCAACGTCCAGGTGCTTCAGGGGCTGACTTTTACGCTGTATCCCGGGAAGGTGACTGCCTTGG
  TGGGACCCAATGGGTCAGGGAAGAGCACCGTGGCCGCCCTGCTGCAGAACCTGTACCAGCCCACCGGGGG
  CAAGGTGCTCCTGGATGGCGAGCTCCTGGTCCAGTATGATCACCACTACCTGCACACGCAGGTGGCCGCA
  GTGGGACAAGAGCCACTGCTATTTGGAAGAAGTTTTCGGGAAAATATTGCCTATGGCCTGACGCGGACTC
  CAACCATGGAGGAAATCACAGCTGTGGCCATGGAGTCCGGAGCCCACGATTTCATCTCTGGATTCCCTCA
  GGGCTATGACACAGAGGTAGGTGAAACTGGGAACCAGCTGTCAGGAGGTCAGCGACAGGCGGTGGCCTTG
  GCTCGAGCCTTGATCCGGAAGCCACGCCTGCTTATCTTGGACGATGCCACCAGTGCCCTGGATGCTGGCA
  ACCAGCTACGGGTCCAGCGGCTCCTGTATGAGAGCCCCGAGTGGGCCTCTCGGACGGTTCTTCTGATCAC
  CCAGCAGCTCAGCCTGGCAGAGCGGGCCCACCACATCCTCTTCCTCAAAGAAGGCTCTGTCTGCGAGCAG
  GGCACCCACCTGCAGCTCATGGAGAGAGGAGGGTGTTACCGGTCCATGGTGGAGGCTCTTGCGGCTCCTT
  CAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204474 representing NM_032055
  Red=Cloning site Green=Tags(s)
  
  MAAHAWPTAALLLLLVDWLLLRPVLPGIFSLLVPEVPLLRVWAVGLSRWAILGLGVRGVLGVTAGARGWL
  AALQPLVAALGLALPGLASFRKLSAWGALREGDNAGLLHWNSRLDAFVLSYVAALPAAALWHKLGGFWAP
  SGHKGAGDMLCRMLGFLDSKKGRLHLVLVLLILSCLGEMAIPFFTGRITDWILQDKTAPSFARNMWLMCI
  LTVASTALEFAGDGIYNITMGHMHSRVHGEVFRAVLHQETGFFLKNPTGSITSRVTEDTSNVCESISDKL
  NLFLWYLGRGLCLLAFMIWGSFYLTVVTLLSLPLLFLLPRRLGKVYQSLAVKVQESLAKSTQVALEALSA
  MPTVRSFANEEGEAQKFRQKLEEMKPLNKKEALAYVTEVWTMSVSGMLLKVGILYLGGQLVVRGAVSSGN
  LVSFVLYQLQFTRAVEVLLSIYPSMQKSVGASEKIFEYLDRTPCSPLSGSLAPLNMKGLVKFQDVSFAYP
  NHPNVQVLQGLTFTLYPGKVTALVGPNGSGKSTVAALLQNLYQPTGGKVLLDGELLVQYDHHYLHTQVAA
  VGQEPLLFGRSFRENIAYGLTRTPTMEEITAVAMESGAHDFISGFPQGYDTEVGETGNQLSGGQRQAVAL
  ARALIRKPRLLILDDATSALDAGNQLRVQRLLYESPEWASRTVLLITQQLSLAERAHHILFLKEGSVCEQ
  GTHLQLMERGGCYRSMVEALAAPSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032055
• ORF Size: 2175 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032055.4, NP_114444.2
• RefSeq Size: 2693 bp
• RefSeq ORF: 2178 bp
• Locus ID: 24811
• Cytogenetics: 20p12
• MW: 79.1 kDa
• Gene Summary: has similarity to multidrug resistant transporters; may transport antigenic peptides across the endoplasmic reticulum membrane in preparation for MHC class I presentation [RGD, Feb 2006]