Tmem175 (NM_001013991) Rat Tagged ORF Clone

CAT#: RR203627

Tmem175 (Myc-DDK-tagged ORF) - Rat transmembrane protein 175 (Tmem175), (10 ug)

Product Images

Circular map for RR203627

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmem175
• Synonyms: RGD1309712
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203627 representing NM_001013991
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGGCTCCAGGTCCAGGAGCAGGCAGTGGATTCCGAGGGAGACTCATCCCTATACAGGAGAGATG
  AGGAGGGGACACAGAGCTCCCACCGCATGCTGGGCTTTAGTGATGCCTTACTATCCATCATCGCCACTGT
  CATGATCCTGCCTGTGACCCACACAGAGATCTCACCAGAACAGCAGTTTGACAAAAGTATACAGAAACTT
  CTAGCAACTAGGATTGCTGTCTACCTCATGACATTTCTAATCGTAACTGTGGCCTGGGCAGCACATACCA
  GATTGTTCCAGGTTGTTGGAAAAATAGATGATACACTTGCCTTGCTCAACCTGGCCTGTATGATGACCAT
  CACCCTTCTGCCCTACACATTTTCGCTAATGGTAACATTCCCTGATGTGCCCCTGGGCATCTTCCTGTTC
  TGCATGTGTGTGATTGCCATTGGATCTGTGCAGGCAATGATCGTGGGTTATGCCTTCCACTTCCCACATC
  TGCTGAATCCACAAATCCAGTGCTCTACACATAGGGCCCTGTCCCGGCGGCACATTCTGCACCTAGTCCT
  CCGTGGCCCAGCACTCTGTTTTGTTGCAGCTGTCTTTTCCCTCTTCTTCTTCCCCTTGTCATACCTGCTG
  ATGGTGACTGTCATCTTCCTCCCTCACATCAGCAAGGCTACCACCTGGTGTAAAGACAAGTTTATGGGCC
  ACAGGGAGTCTCCAGCTCACAATGTAGAGCCCTTCAGTATTGACCTGCATGCGCCCCTCAGCAAAGAACG
  GGTGGAAGCTTTCAGTGACGGTGTCTATGCCATTGTGGCCACACTCCTCATCTTGGACATCTGTGAGGAC
  AATGTTCCGGATCCCAAGGATGTGCAACAGAAGTTCAGTGGCAGCCTTGTGGCTGCCCTGGGTGCATATG
  GGCCACAGTTCCTGGCATACTTCGGCTCCTTCGCCACAGTGGGTCTGCTCTGGTTTGCCCACCACTCACT
  CTTCCTGCATGTCCGGAAGGCTACACAGACCATGGGGTTGTTCAACATACTGTCACTGGCCTTTGTGGGG
  GGCCTCCCTCTGGCCTACCAGCAGACCTCGGCGTTTGCCCGGCAACCCCGTGATGAACTTGAGCGTGTGC
  GTGTCAGCTGTGCCATCATCTTCTTTGCCAGCATCTTCCAGTTTGCCATCTGGACTACAGCCCTGCTGCA
  CCAGAGAGAAACACTGCAGCCAGCTGTGCAGTTTGGTGGGCAGGAACATGCTTTCATGTTTGCCAAGCTC
  GCACTGTACCCCTGTGCCAGCCTGCTGGCCTTTGCCGCCACCTGCCTACTGAGTCGGTTCAGCACAGCCA
  TCTTCCACCTCATGCAGATTGCAGTGCCCTTTGCGTTCCTGTTGCTCCGGCTCCTGGTACGCCTTGCCCT
  GGCAGGCCTTCAAGTCCTATGGGACCTCTGGCCAGAGCGTCCCCAACAAGACCAGGGTGAACCCGAGACA
  CAGTCCCAGCTCTTGCCTGCCTCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203627 representing NM_001013991
  Red=Cloning site Green=Tags(s)
  
  MSRLQVQEQAVDSEGDSSLYRRDEEGTQSSHRMLGFSDALLSIIATVMILPVTHTEISPEQQFDKSIQKL
  LATRIAVYLMTFLIVTVAWAAHTRLFQVVGKIDDTLALLNLACMMTITLLPYTFSLMVTFPDVPLGIFLF
  CMCVIAIGSVQAMIVGYAFHFPHLLNPQIQCSTHRALSRRHILHLVLRGPALCFVAAVFSLFFFPLSYLL
  MVTVIFLPHISKATTWCKDKFMGHRESPAHNVEPFSIDLHAPLSKERVEAFSDGVYAIVATLLILDICED
  NVPDPKDVQQKFSGSLVAALGAYGPQFLAYFGSFATVGLLWFAHHSLFLHVRKATQTMGLFNILSLAFVG
  GLPLAYQQTSAFARQPRDELERVRVSCAIIFFASIFQFAIWTTALLHQRETLQPAVQFGGQEHAFMFAKL
  ALYPCASLLAFAATCLLSRFSTAIFHLMQIAVPFAFLLLRLLVRLALAGLQVLWDLWPERPQQDQGEPET
  QSQLLPASC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013991
• ORF Size: 1497 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013991.1, NP_001014013.1
• RefSeq Size: 1836 bp
• RefSeq ORF: 1500 bp
• Locus ID: 305623
• UniProt ID: Q6AY05
• Cytogenetics: 14p22
• MW: 55.7 kDa
• Gene Summary: Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel.[UniProtKB/Swiss-Prot Function]