Abcb6 (NM_080582) Rat Tagged ORF Clone

CAT#: RR203264

Abcb6 (Myc-DDK-tagged ORF) - Rat ATP-binding cassette, sub-family B (MDR/TAP), member 6 (Abcb6), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR203264

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcb6
• Synonyms: MTABC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203264 representing NM_080582
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGACTGTGGGCAACTACTGCGAGGCCGAAGGGCCGGCGGGGCCGGCATGGACTCAGAATGGCTTGA
  GTCCCTGCTTCTTCTACACTCTAGTGCCCTCGACACTCATGACTTTGGGGGTACTGGCCTTGGTGCTGGT
  TCTTCCCTGCAGGCGTCGGGAGGTGCCTGCTGGCACAGAGGAGCTATCTTGGGCGGCTGGCCCTCGGGTC
  GCTCCCTATGCGCTGCAGCTGTCTCTGGCGATACTTCAGATGGCACTGCCCCTGGCCAGTCTGGCTGGCC
  GAGTGGGCACTGCTCGGGGGGTCCGCCTGCCAGGTTACCTACTGCTGGCTTCTGTGCTGGAGAGTCTAGC
  CAGTGCCTGTGGCTTGTGGCTGCTCGTGGTGGAACGGAGCCAGGCACGGCAGAGTCTGGCAATGGGCGTC
  TGGATGAAGTTCAGGCATAGCTTGGGTCTTCTGCTCCTCTGGACTGTGACATTTGCAGCCGAGAACTTGG
  TCCTGGTATCTTGGAACAGCCCACAGTGGTGGTGGTCCAGGGCAGATCTGGGCCAGCAGGTTCAATTTGG
  CCTATGGGTCCTGCGTTATATGACCTCTGGAGGGCTATTTATCCTGGGGCTCTGGGCCCCTGGACTTCGC
  CCCCAGTCTTATACCCTACATGTTAATGAAGAAGACCAAGATGGAGGAAGGAACCAGGGCCGCTCAACAG
  ACCCACGATCCACTTGGAGAGACCTCGGCAGGAAGCTTCGACTGCTGAGTGGCTACTTGTGGCCTCGGGG
  GAGTCCATCTCTGCAGCTGACTGTGCTCCTATGCATGGGCCTCATGGGGTTGGACCGAGCACTCAATGTG
  TTGGTCCCCATCTTCTATAGGGACATTGTGAACTTACTGACTTCGAAGGCTCCTTGGAGCTCCCTGGCCT
  GGACCGTTACCACCTATGTCTTCCTCAAGTTCCTGCAGGGGGGTGGCACTGGCAGTACAGGTTTTGTGAG
  CAACCTTCGAACCTTCCTGTGGATCCGGGTGCAGCAGTTCACGTCCCGAGGTGTGGAACTCCGTCTCTTC
  TCTCATCTGCATGAACTCTCACTGCGCTGGCACCTGGGCCGTCGGACTGGGGAGGTGCTTCGGATTGTGG
  ACCGGGGAACATCCAGTGTCACAGGACTGCTCAGCTACCTGGTGTTCAACATCATCCCCACCCTGGCCGA
  CATCATCATTGGCATCATCTACTTCAGCATGTTCTTCAATGCCTGGTTTGGCCTCATTGTGTTCCTGTGC
  ATGAGTCTTTACCTCATCCTCACTATTATGGTCACTGAGTGGAGAGCCAAGTTTCGCCGTGATATGAACA
  CACAGGAAAATGCCACCCGGGCACGAGCAGTAGACTCTCTGCTAAACTTTGAGACGGTGAAATACTACAA
  TGCGGAGGGTTATGAGTTAGAACGCTATCGAGAGGCCATCCTCAAGTTTCAGGGTTTGGAGTGGAAGTCA
  ACGGCTTCACTGGTTTTACTGAATCAGACCCAGAACATGGTGATTGGATTTGGGCTCCTTGCTGGCTCCT
  TGCTTTGTGCATACTTCGTCAGCGAGCGACGGCTACAGGTTGGGGACTTTGTGCTTTTTGGCACTTATAT
  CACCCAACTGTACATGCCTCTCAACTGGTTCGGCACCTACTACAGAATGATCCAGACTAACTTCATTGAC
  ATGGAGAACATGTTTGACTTGTTGAAAGAGGAAACAGAGGTGAAGGACGTCCCTGGAGCAGGGCCCCTTC
  GTTTTCATAAGGGCCGGGTTGAGTTTGAAAATGTGCACTTCAGCTACGCTGATGGGCGGGAGACCTTACA
  AGATGTGTCCTTCACTGTGATGCCTGGACAGACGGTGGCTCTGGTAGGCCCATCTGGGGCAGGAAAGAGC
  ACAATTTTACGCCTGCTGTTTCGCTTCTATGACATCAGCTCTGGCTGCATCCGAATAGATGGACAGGACA
  TTTCACAGGTAACCCAGATCTCTCTCCGGTCTCACATTGGAGTTGTGCCCCAGGACACTGTCCTCTTCAA
  CGACACCATTGCCAACAATATCCGCTATGGCCGTGTTACAGCTGGGGACAGTGAGATACAGGCTGCTGCT
  CAGGCTGCGGGCATCCATGATGCCATCTTGTCTTTCCCTGAAGGGTATGAGACACAGGTTGGGGAGCGGG
  GTCTGAAGCTGAGTGGTGGCGAGAAGCAGCGAGTGGCCATTGCCCGCACCATCCTTAAGGCTCCTGACAT
  CATTCTGCTGGATGAGGCAACATCAGCACTGGATACATCTAATGAGAGAGCCATCCAGGCCTCTCTGGCC
  AAAGTCTGCACCAACCGCACCACCATCGTAGTAGCACACAGGCTCTCAACTGTGGTCAATGCTGACCAGA
  TTCTTGTCATCAAGGATGGCTGCATCATAGAGAGAGGAAGGCACGAAGCCCTGCTATCCCGAGGTGGCGT
  GTATGCTGAGATGTGGCAGCTGCAGCAGCAAGGACAAGAAACTGTCCCTGAAGACTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203264 representing NM_080582
  Red=Cloning site Green=Tags(s)
  
  MVTVGNYCEAEGPAGPAWTQNGLSPCFFYTLVPSTLMTLGVLALVLVLPCRRREVPAGTEELSWAAGPRV
  APYALQLSLAILQMALPLASLAGRVGTARGVRLPGYLLLASVLESLASACGLWLLVVERSQARQSLAMGV
  WMKFRHSLGLLLLWTVTFAAENLVLVSWNSPQWWWSRADLGQQVQFGLWVLRYMTSGGLFILGLWAPGLR
  PQSYTLHVNEEDQDGGRNQGRSTDPRSTWRDLGRKLRLLSGYLWPRGSPSLQLTVLLCMGLMGLDRALNV
  LVPIFYRDIVNLLTSKAPWSSLAWTVTTYVFLKFLQGGGTGSTGFVSNLRTFLWIRVQQFTSRGVELRLF
  SHLHELSLRWHLGRRTGEVLRIVDRGTSSVTGLLSYLVFNIIPTLADIIIGIIYFSMFFNAWFGLIVFLC
  MSLYLILTIMVTEWRAKFRRDMNTQENATRARAVDSLLNFETVKYYNAEGYELERYREAILKFQGLEWKS
  TASLVLLNQTQNMVIGFGLLAGSLLCAYFVSERRLQVGDFVLFGTYITQLYMPLNWFGTYYRMIQTNFID
  MENMFDLLKEETEVKDVPGAGPLRFHKGRVEFENVHFSYADGRETLQDVSFTVMPGQTVALVGPSGAGKS
  TILRLLFRFYDISSGCIRIDGQDISQVTQISLRSHIGVVPQDTVLFNDTIANNIRYGRVTAGDSEIQAAA
  QAAGIHDAILSFPEGYETQVGERGLKLSGGEKQRVAIARTILKAPDIILLDEATSALDTSNERAIQASLA
  KVCTNRTTIVVAHRLSTVVNADQILVIKDGCIIERGRHEALLSRGGVYAEMWQLQQQGQETVPEDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080582
• ORF Size: 2508 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080582.1, NP_542149.1
• RefSeq Size: 2848 bp
• RefSeq ORF: 2511 bp
• Locus ID: 140669
• UniProt ID: O70595
• Cytogenetics: 9q33
• MW: 93.3 kDa
• Gene Summary: ATP-binding cassette (ABC) half-transporter that together with its dimerization partner alters the localization of toxic substances [RGD, Feb 2006]