Csnk2a1 (NM_053824) Rat Tagged ORF Clone

CAT#: RR203076

Csnk2a1 (Myc-DDK-tagged ORF) - Rat casein kinase 2, alpha 1 polypeptide (Csnk2a1), (10 ug)

Product Images

Circular map for RR203076

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Csnk2a1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203076 representing NM_053824
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGACCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAACACACACAGACCCCGAGAGTACT
  GGGACTATGAATCACATGTGGTGGAATGGGGAAATCAAGATGACTACCAGCTTGTTCGAAAATTAGGCAG
  GGGCAAATACAGTGAAGTGTTTGAAGCCATCAATATCACAAATAATGAAAAAGTTGTTGTTAAAATTCTC
  AAGCCAGTAAAAAAGAAGAAAATTAAGCGTGAAATAAAGATTTTGGAGAATTTAAGAGGTGGGCCCAACA
  TCATCACACTTGCAGACATTGTGAAAGACCCTGTGTCTCGAACCCCTGCCTTGGTTTTTGAACATGTAAA
  CAACACAGACTTCAAGCAATTGTACCAGACGTTAACAGACTATGACATTCGATTTTACATGTATGAAATT
  CTGAAAGCCCTGGATTATTGTCACAGCATGGGGATTATGCACAGAGACGTGAAACCGCATAATGTCATGA
  TTGATCATGAGCACAGAAAGCTTCGGCTAATAGATTGGGGTTTAGCAGAGTTTTACCATCCTGGCCAAGA
  GTATAATGTCCGAGTTGCTTCCCGATATTTCAAAGGTCCAGAGCTACTTGTAGATTATCAGATGTACGAT
  TATAGTTTGGATATGTGGAGCTTGGGTTGTATGCTGGCAAGTATGATCTTCCGGAAGGAGCCATTTTTCC
  ATGGACATGACAATTATGATCAGTTGGTGAGGATAGCCAAGGTTCTGGGAACGGAAGATTTATATGACTA
  TATTGACAAGTACAACATTGAATTAGATCCACGTTTCAACGATATCTTGGGCAGACACTCCCGTAAGCGA
  TGGGAACGCTTTGTCCACAGTGAAAACCAGCACCTTGTCAGCCCCGAGGCCTTGGATTTTCTGGACAAGC
  TGCTGCGATACGACCACCAGTCTCGGCTCACTGCAAGAGAGGCCATGGAGCACCCTTACTTCTACACTGT
  CGTGAAGGACCAGGCTCGAATGAGTTCGGCTGGCATGGCAGGGGGCAGCACACCTGTCAGTAGCGCCAAT
  ATGATGTCAGGGATTTCTTCAGTGCCAACCCCTTCACCCCTGGGACCTCTGGCAGGCTCACCCGTGATTG
  CTGCTGCCAACTCACTTGGGATACCTGTACCAGCTGCCGCTGGCGCTCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203076 representing NM_053824
  Red=Cloning site Green=Tags(s)
  
  MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKIL
  KPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEI
  LKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYD
  YSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKR
  WERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMSSAGMAGGSTPVSSAN
  MMSGISSVPTPSPLGPLAGSPVIAAANSLGIPVPAAAGAQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053824
• ORF Size: 1173 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053824.2, NP_446276.1
• RefSeq Size: 1546 bp
• RefSeq ORF: 1176 bp
• Locus ID: 116549
• UniProt ID: P19139
• Cytogenetics: 3q41
• MW: 45.1 kDa
• Gene Summary: Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythms. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Feb 2014]