Dcc (NM_012841) Rat Tagged ORF Clone

CAT#: RR202446

Dcc (Myc-DDK-tagged ORF) - Rat deleted in colorectal carcinoma (Dcc), (10 ug)

Product Images

Circular map for RR202446

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dcc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202446 representing NM_012841
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAATAGTCTTGGATGTGTCTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCA
  GCGCGCATCTTCAAGTCACGGGTTTCCAAATTAAACCTTTCACGTCACTTCATTTTGTGTCTGAACCTTC
  TGATGCTGTCACAATGCGGGGAGGAAACGTCCTGCTCAACTGCTCTGCAGAGTCTGACCGCGGAGTCCCA
  GTCATCAAGTGGAAGAAAGATGGCCTTATCCTGGCCTTGGGAATGGATGACAGAAAGCAGCAACTTCCTA
  ATGGGTCTCTGTTGATACAAAACATACTTCATTCCAGACACCATAAACCGGATGAAGGACTTTACCAATG
  CGAGGCATCCTTAGGAGACTCTGGCTCAATTATTAGTCGGACAGCAAAAGTTATGGTAGCAGGACCACTG
  AGGTTCCTCTCCCAGACAGAATCTATCACAGCCTTCATGGGAGATACAGTGTTACTTAAGTGTGAAGTCA
  TTGGGGACCCCATGCCAACAATACACTGGCAGAAGAATCAACAAGACCTCAACCCGATCCCAGGAGACTC
  GCGTGTGGTCGTCTTGCCCTCCGGAGCATTGCAGATCAGCCGACTTCAACCAGGAGACAGTGGAGTCTAC
  CGATGCTCAGCTCGGAATCCAGCCAGCACAAGAACAGGAAATGAAGCCGAAGTCAGGATTTTATCAGACC
  CAGGCCTGCATAGGCAGCTGTATTTTCTGCAGAGACCATCCAATGTGATAGCCATTGAAGGGAAAGATGC
  TGTCCTGGAGTGTTGTGTTTCTGGCTATCCTCCACCAAGTTTCACCTGGTTACGGGGCGAGGAGGTCATC
  CAACTCAGGTCTAAAAAGTATTCGTTGTTGGGTGGGAGCAATTTGCTCATCTCTAATGTGACAGATGATG
  ACAGTGGGACCTATACGTGCGTTGTCACATATAAGAATGAGAATATTAGTGCCTCTGCAGAGCTCACGGT
  CTTGGTTCCGCCATGGTTTTTAAATCACCCTTCCAACCTCTATGCCTATGAAAGCATGGACATTGAGTTT
  GAATGTGCAGTGTCCGGGAAGCCTGTGCCCACTGTGAATTGGATGAAGAATGGAGATGTGGTGATTCCTA
  GTGATTACTTTCAGATAGTGGGAGGAAGCAACTTACGAATCCTTGGGGTGGTGAAATCAGATGAAGGCTT
  TTATCAATGTGTGGCTGAAAATGAGGCTGGCAATGCCCAAAGTAGTGCCCAGCTGATTGTCCCCAAGCCT
  GCCATCCCAAGCTCCAGCATCCTCCCTTCTGCTCCCAGAGATGTGGTCCCTGTCTTGGTTTCTAGTCGCT
  TTGTCCGTTTGAGCTGGCGTCCACCTGCCGAAGCCAAAGGAAACATTCAAACATTCACAGTCTTTTTCTC
  CAGAGAGGGTGACAACAGGGAGCGAGCTTTGAATACAACCCAGCCTGGGTCCCTTCAGCTTACTGTGGGA
  AACTTGAAACCAGAAGCCATGTATACCTTCAGAGTTGTTGCCTACAATGAGTGGGGACCAGGGGAGAGCT
  CTCAACCCATCAAGGTGGCCACTCAGCCTGAGTTGCAAGTTCCAGGGCCAGTAGAAAACCTGCACGCTGT
  ATCTGCCTCTCCTACCTCAATTCTTATTACATGGGAACCCCCTGCCTATGCAAATGGTCCGGTTCAAGGT
  TACAGATTGTTTTGCACTGAGGTGTCTACAGGAAAAGAACAGAATATAGAGGTTGATGGACTATCTTATA
  AACTGGAAGGCCTGAAAAAATTCACGGAATATACTCTTCGATTCCTTGCTTATAATCGTTATGGTCCGGG
  AGTGTCTACCGATGACATAACAGTCGTTACACTTTCAGATGTGCCAAGTGCTCCACCTCAGAACGTCTCT
  CTGGAAGTGGTTAATTCAAGGAGTATCAAAGTAAGCTGGCTCCCTCCTCCATCGGGAACACAAAATGGAT
  TTATTACTGGCTATAAAATTCGACACAGAAAGACTACCCGCAGGGGTGAGATGGAAACACTGGAACCCAA
  TAACCTCTGGTACCTCTTCACAGGATTGGAGAAGGGGAGTCAGTACAGTTTCCAAGTGTCAGCCATGACT
  GTCAATGGCACTGGACCACCTTCTAACTGGTACACAGCAGAAACCCCAGAGAACGACTTAGACGAGTCTC
  AAGTTCCTGACCAGCCAAGTTCTCTCCATGTGAGGCCCCAGACAAACTGCATCATCATGAGTTGGACTCC
  ACCTCTCAACCCAAACATCGTGGTGAGAGGCTATATTATCGGATATGGTGTTGGCAGCCCTTATGCCGAG
  ACAGTGCGTGTAGACAGTAAGCAGCGGTACTATTCCATCGAGAGGCTGGAGTCGAGTTCTCATTATGTAA
  TCTCCTTAAAAGCTTTCAACAATGCTGGAGAAGGTGTCCCTCTCTACGAAAGTGCCACTACCCGTTCCAT
  AACAGACCCCACTGACCCCGTTGATTATTATCCTTTGCTTGATGATTTCCCCACCTCGGGCCCAGATGTC
  TCCACCCCCATGCTCCCACCAGTAGGTGTGCAGGCTGTGGCTCTTACCCACGAGGCTGTGAGGGTCAGCT
  GGGCAGACAACTCTGTCCCGAAGAACCAGAAGACATCCGACGTTCGGCTTTACACGGTTCGGTGGAGGAC
  CAGCTTTTCCGCCAGTGCCAAGTACAAGTCAGAAGACACAACGTCTCTCAGCTACACAGCGACTGGCCTC
  AAGCCAAATACCATGTATGAGTTCTCAGTCATGGTGACAAAGAACAGAAGGTCAAGCACGTGGAGCATGA
  CTGCACACGCCACCACCTACGAAGCTGCCCCAACTTCTGCTCCCAAGGATTTGACAGTCATTACTCGTGA
  GGGGAAGCCTCGTGCTGTCATTGTGAGCTGGCAGCCTCCCTTGGAAGCCAATGGGAAAATCACCGCTTAC
  ATCTTGTTTTATACCTTGGACAAGAATATACCCATCGATGACTGGATTATGGAGACTATCAGCGGTGACC
  GGCTTACCCATCAGATCATGGATCTGAGCCTGGACACTATGTATTACTTTCGAATTCAAGCACGGAACGC
  TAAAGGCGTGGGGCCCCTCTCGGATCCCATCCTCTTCAGGACTTTGAAAGTGGAACACCCTGACAAAATG
  GCTAATGACCAAGGTCGTCATGGAGATGGAGGTTATTGGCCAGTTGATACTAACTTGATTGATAGAAGCA
  CTCTCAACGAACCACCCATTGGACAAATGCACCCCCCACATGGCAGTGTAACTCCTCAAAAGAACAGTAA
  CCTGCTTGTCATAACCGTGGTCACCGTGGGCGTTCTCACCGTGTTGGTTGTGGTCATTGTAGCAGTGATT
  TGTACCCGGCGCTCTTCAGCCCAGCAAAGGAAGAAACGGGCCACGCACAGTGCGAGCAAGAGGAAGGGCA
  GTCAGAAGGACCTCCGGCCTCCGGATCTTTGGATACATCATGAAGAAATGGAAATGAAAAATATCGAGAA
  GCCGGCAGGAACCGACCCTGCAGGAAGAGGCTCCCCCATCCAGAGTTGCCAAGACCTCACACCAGTCAGC
  CACAGCCAGTCAGAAAGCCAGATGGGAAGCAAAAGTGCCTCCCATTCAGGTCAAGATACCGAGGAGGCAG
  GGAGCTCCATGTCTACTCTAGAAAGGTCCCTTGCAGCACGGCGGGCCACGAGGACCAAGCTCATGATTCC
  CATGGAGGCCCAGTCCAATAATCCTGCTGTCGTGAGTGCCATCCCTGTACCAACCCTAGAAAGTGCCCAG
  TATCCAGGAATCCTCCCGTCTCCCACATGTGGATATCCGCATCCACAGTTCACTCTCCGGCCAGTACCAT
  TCCCAACACTGTCTGTGGACCGAGGTTTCGGAGCAGGAAGAACTGTGAGTGAAGGACCAACAGCACAACA
  ACAACCCATGCTGCCCCCAGCTCAGCCTGAACATCCCAGCAGCGAAGAAGCCCCCAGCAGAACCATCCCT
  ACAGCCTGTGTGCGGCCAACTCACCCACTCCGAAGTTTTGCAAACCCTTTGCTACCTCCACCTATGAGTG
  CAATAGAACCAAAAGTCCCTTACACACCGCTTTTGTCGCAGCCAGGACCCACCCTTCCCAAGACTCATGT
  TAAAACGGCCTCCCTTGGGCTAGCAGGAAAGGCAAGGTCCCCTTTGCTTCCCGTATCTGTGCCAACAGCC
  CCTGAAGTGTCTGAAGAGAGTCACAAGCCAACAGAGGATCCAGCCAGTGTATATGAACAGGATGACTTGA
  GCGAACAAATGGCGAGTTTGGAAGGGCTAATGAAGCAACTCAATGCCATCACGGGCTCCGCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202446 representing NM_012841
  Red=Cloning site Green=Tags(s)
  
  MENSLGCVWVPKLAFVLFGASLLSAHLQVTGFQIKPFTSLHFVSEPSDAVTMRGGNVLLNCSAESDRGVP
  VIKWKKDGLILALGMDDRKQQLPNGSLLIQNILHSRHHKPDEGLYQCEASLGDSGSIISRTAKVMVAGPL
  RFLSQTESITAFMGDTVLLKCEVIGDPMPTIHWQKNQQDLNPIPGDSRVVVLPSGALQISRLQPGDSGVY
  RCSARNPASTRTGNEAEVRILSDPGLHRQLYFLQRPSNVIAIEGKDAVLECCVSGYPPPSFTWLRGEEVI
  QLRSKKYSLLGGSNLLISNVTDDDSGTYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEF
  ECAVSGKPVPTVNWMKNGDVVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQSSAQLIVPKP
  AIPSSSILPSAPRDVVPVLVSSRFVRLSWRPPAEAKGNIQTFTVFFSREGDNRERALNTTQPGSLQLTVG
  NLKPEAMYTFRVVAYNEWGPGESSQPIKVATQPELQVPGPVENLHAVSASPTSILITWEPPAYANGPVQG
  YRLFCTEVSTGKEQNIEVDGLSYKLEGLKKFTEYTLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNVS
  LEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEMETLEPNNLWYLFTGLEKGSQYSFQVSAMT
  VNGTGPPSNWYTAETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAE
  TVRVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLDDFPTSGPDV
  STPMLPPVGVQAVALTHEAVRVSWADNSVPKNQKTSDVRLYTVRWRTSFSASAKYKSEDTTSLSYTATGL
  KPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPKDLTVITREGKPRAVIVSWQPPLEANGKITAY
  ILFYTLDKNIPIDDWIMETISGDRLTHQIMDLSLDTMYYFRIQARNAKGVGPLSDPILFRTLKVEHPDKM
  ANDQGRHGDGGYWPVDTNLIDRSTLNEPPIGQMHPPHGSVTPQKNSNLLVITVVTVGVLTVLVVVIVAVI
  CTRRSSAQQRKKRATHSASKRKGSQKDLRPPDLWIHHEEMEMKNIEKPAGTDPAGRGSPIQSCQDLTPVS
  HSQSESQMGSKSASHSGQDTEEAGSSMSTLERSLAARRATRTKLMIPMEAQSNNPAVVSAIPVPTLESAQ
  YPGILPSPTCGYPHPQFTLRPVPFPTLSVDRGFGAGRTVSEGPTAQQQPMLPPAQPEHPSSEEAPSRTIP
  TACVRPTHPLRSFANPLLPPPMSAIEPKVPYTPLLSQPGPTLPKTHVKTASLGLAGKARSPLLPVSVPTA
  PEVSEESHKPTEDPASVYEQDDLSEQMASLEGLMKQLNAITGSAF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012841
• ORF Size: 4335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012841.1, NP_036973.1
• RefSeq Size: 4924 bp
• RefSeq ORF: 4338 bp
• Locus ID: 25311
• UniProt ID: Q63155
• Cytogenetics: 18q12.1
• MW: 157.9 kDa
• Gene Summary: transmembrane protein of the immunoglobulin superfamily that plays dual roles as a receptor for the chemoattractant netrin-1 and possibly other signalling pathways, and as a gamma-secretase-dependent transcription coactivator [RGD, Feb 2006]