Btd (NM_001012047) Rat Tagged ORF Clone

CAT#: RR201713

Btd (Myc-DDK-tagged ORF) - Rat biotinidase (Btd), (10 ug)

Product Images

Circular map for RR201713

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Btd
• Synonyms: MGC109548
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201713 representing NM_001012047
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGAGCCCGCACTGCGCATGCGCTCGTCTTCCTCCTCGGCTGTTCTGCGCTTGCGTTGGGCGTCT
  GCTCAGCCTCCCTGGAGCACGGCGAGGCTGAGTACTACGTGGCTGCCGTGTACGAACACCGATCGGTCCT
  GAGCCCAAACCCCCTGGAGCTCAGCAGCCGCCAGCAGGCCCTGGAGCTCATGAAGCAGAACCTCGACGTC
  TATGAACAGCAAGTGATGGCTGCAGCCCAGAAGGGTGCGCACATTATAGTGTTCCCAGAAGACGGTATCC
  ACGGATTCAACTTCACAAGAACATCCATTTACCCATTCTTGGACTTGATGCCATCTCCCAGGCTGGTCAG
  TTGGAACCCGTGTCTGGAGCCCTTTCGCTTCAATGACACGGAGGTCCTCCAGCGCCTGAGTTGCATGGCC
  ATCAAGGGAAAGATGTTCTTGGTGGCCAATCTTGGGACAAAACAGCCTTGCCTCGGCAGCGACCCTGGGT
  GCCCGCAGGACGGGAGATACCAGTTTAACACAAACGTCGCGTTCAGTGACAACGGAACCCTCGTCGGCCG
  CTACCGTAAGCACAACCTGTACTTTGAGGAAGCCTTCGATAGCCCTGCTGACGTGGACCTCACCACCTTT
  GATACGCCGTTCGCGGGCAAGTTCGGTATGTTCACTTGCTTTGATATCCTGTTCTTTGACCCCGCGGTCC
  GGCTCCTCAGAGACTTTGAGGTAAAGCACATTGCGTACCCTACGGCCTGGATGAACCAGCTGCCGCTCTT
  GGCAGCCATTGAAATCCAGAAAGCATTCGCCACCGCCTTCGGCGTCACCGTTCTGGCAGCTAACATCCAC
  CACCCGACTCTGGGGATGACGGGCAGTGGCATACACACCCCTCTGAAGTCCTTTTGGTACCATAACATGG
  ACGACCCCGAAGGTCACCTTATAATTGCCCGGGTAGCCACAAACCCACAGGGCCTCGTTGGGACAGAGAA
  TACAACTAGTGAAATGGATCCGTCCCATAGGAAGTTCTTAAAAGTCTTGTCTGGGGATCCGTACTGTGAG
  AAGGATGCCCAGGAAGTGCGCTGCGACGAGGCTGCCAAGTGGAACCTGAACGCACCACCTACCTTCCACT
  CGGAGATGATGTATGACAATTTCACCCTGGTCCCCGTCTGGGGAAAGGAAGGCCACCTCCAGGTGTGCTC
  CAACAGTCTCTGTTGCCACTTACTTTTCGAGAGGCCCGCCCTGTCCAAAGAGCTATATGCCCTGGGTGTC
  TTTGACGGACTCCACACAGTGCACGGTACCTACTACGTTCAAGCCTGTGCCCTGGTCAAGTGCGGGGGCG
  CTGGCTTTGAAACCTGCGGGCAGGAGATCTCGGAGGCCGAGGGCTTGTTTGACTTTCATCTGTGGGGGAA
  CTTCAGCACCTTGTATGTCTTCCCTTTATTTCTCACCTCGGGGATGACCCTAGACACCCCTGACCAGCTC
  GGGTGGGAGAGCGACCACTATTTCTTGAGGAAGAGGGGACTGTCCTCCGGCCTGGTGACGGCAGCTCTCT
  ATGGGCGGTTGTATGAGAGGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201713 representing NM_001012047
  Red=Cloning site Green=Tags(s)
  
  MSGARTAHALVFLLGCSALALGVCSASLEHGEAEYYVAAVYEHRSVLSPNPLELSSRQQALELMKQNLDV
  YEQQVMAAAQKGAHIIVFPEDGIHGFNFTRTSIYPFLDLMPSPRLVSWNPCLEPFRFNDTEVLQRLSCMA
  IKGKMFLVANLGTKQPCLGSDPGCPQDGRYQFNTNVAFSDNGTLVGRYRKHNLYFEEAFDSPADVDLTTF
  DTPFAGKFGMFTCFDILFFDPAVRLLRDFEVKHIAYPTAWMNQLPLLAAIEIQKAFATAFGVTVLAANIH
  HPTLGMTGSGIHTPLKSFWYHNMDDPEGHLIIARVATNPQGLVGTENTTSEMDPSHRKFLKVLSGDPYCE
  KDAQEVRCDEAAKWNLNAPPTFHSEMMYDNFTLVPVWGKEGHLQVCSNSLCCHLLFERPALSKELYALGV
  FDGLHTVHGTYYVQACALVKCGGAGFETCGQEISEAEGLFDFHLWGNFSTLYVFPLFLTSGMTLDTPDQL
  GWESDHYFLRKRGLSSGLVTAALYGRLYERN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012047
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012047.1, NP_001012047.1
• RefSeq Size: 1999 bp
• RefSeq ORF: 1566 bp
• Locus ID: 306262
• UniProt ID: Q5FVF9
• Cytogenetics: 16p16
• MW: 58 kDa
• Gene Summary: Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.[UniProtKB/Swiss-Prot Function]