Ctnnb1 (NM_053357) Rat Tagged ORF Clone

CAT#: RR200930

Ctnnb1 (Myc-DDK-tagged ORF) - Rat catenin (cadherin associated protein), beta 1 (Ctnnb1), (10 ug)

Product Images

Circular map for RR200930

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ctnnb1
• Synonyms: Catnb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200930 representing NM_053357
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACTCAAGCTGACCTCATGGAGTTGGACATGGCCATGGAGCCAGACAGAAAGGCCGCTGTCAGCC
  ACTGGCAGCAGCAATCTTACCTGGATTCTGGAATCCACTCTGGTGCCACCACCACAGCTCCTTCCCTGAG
  TGGCAAGGGCAATCCTGAGGAAGAAGATGTGGACACCTCCCAAGTCCTTTATGAGTGGGAGCAAGGCTTT
  TCCCAGTCCTTCACGCAAGAGCAAGTAGCTGACATTGACGGTCAGTACGCCATGACTCGGGCTCAGAGGG
  TCCGAGCTGCCATGTTCCCTGAGACACTAGATGAGGGCATGCAGATCCCATCCACGCAGTTTGATGCCGC
  TCATCCCACTAATGTCCAGCGCTTGGCTGAACCGTCACAGATGCTGAAACATGCAGTTGTCAATTTGATT
  AACTATCAGGATGACGCGGAACTTGCCACCCGTGCAATTCCTGAGCTGACCAAACTGCTAAATGACGAGG
  ACCAGGTGGTCGTTAATAAAGCTGCTGTTATGGTTCACCAGCTTTCCAAAAAGGAAGCTTCCAGACACGC
  CATCATGCGCTCCCCTCAGATGGTGTCTGCCATAGTGCGCACCATGCAGAATACAAATGACGTAGAAACA
  GCCCGTTGTACCGCTGGGACCCTACACAACCTTTCCCACCATCGAGAGGGCTTGTTGGCCATCTTTAAAT
  CTGGCGGCATCCCAGCGCTGGTGAAAATGCTTGGGTCGCCAGTGGATTCCGTACTGTTCTACGCCATCAC
  CACGCTGCATAATCTCCTGCTACATCAGGAAGGAGCTAAAATGGCAGTGCGCCTAGCTGGTGGGCTGCAG
  AAAATGGTTGCTTTGCTCAACAAAACAAACGTGAAGTTCTTGGCTATTACGACAGACTGCCTTCAGATCT
  TAGCTTACGGCAATCAGGAAAGCAAGCTCATCATTCTGGCCAGTGGTGGACCCCAAGCCTTAGTAAACAT
  AATGAGAACCTACACGTACGAGAAGCTCCTGTGGACCACAAGCAGAGTGCTGAAGGTGCTGTCTGTCTGC
  TCTAGCAACAAGCCGGCCATCGTGGAAGCTGGTGGGATGCAGGCACTGGGGCCTCACCTGACAGACCCGA
  GTCAGCGACTTGTTCAAAACTGTCTTTGGACTCTGAGAAACTTGTCCGATGCAGCGACTAAGCAGGAAGG
  GATGGAAGGCCTCCTTGGGACTCTAGTGCAGCTTCTGGGTTCTGATGATATAAATGTGGTCACCTGCGCA
  GCTGGAATTCTCTCTAACCTCACTTGCAATAATTACAAAAACAAGATGATGGTGTGCCAAGTGGGTGGCA
  TAGAGGCTCTTGTGCGCACTGTCCTTCGTGCTGGTGACAGGGAGGACATTACCGAGCCTGCCATCTGTGC
  TCTTCGTCATCTGACCAGCCGACATCAGGAAGCTGAGATGGCCCAGAATGCCGTTCGCCTTCATTATGGA
  CTACCTGTTGTGGTTAAACTCCTGCACCCACCATCCCACTGGCCTCTGATAAAGGCAACTGTTGGATTGA
  TCCGAAACCTTGCCCTTTGCCCAGCAAATCATGCGCCTTTGCGGGAACAGGGTGCGATCCCACGACTAGT
  TCAGCTGCTTGTACGAGCACATCAGGACACCCAGCGGCGCACGTCCATGGGTGGAACACAGCAGCAGTTC
  GTGGAGGGCGTCCGCATGGAGGAGATAGTTGAAGGGTGCACTGGGGCTCTCCACATCCTCGCTCGGGATG
  TTCACAACCGGATTGTGATCCGAGGACTCAATACCATTCCACTGTTTGTGCAGTTGCTTTATTCTCCCAT
  TGAAAATATCCAAAGAGTAGCTGCAGGGGTCCTCTGTGAACTTGCTCAGGACAAGGAGGCTGCAGAGGCC
  ATTGAGGCTGAGGGAGCCACAGCTCCCCTGACAGAGTTGCTCCACTCCAGGAATGAAGGCGTGGCAACAT
  ATGCGGCTGCTGTTCTATTCCGAATGTCTGAGGACAAGCCACAGGACTACAAGAAACGGCTTTCGGTTGA
  GCTGACCAGTTCCCTCTTCAGGACAGAGCCAATGGCTTGGAATGAGACTGCTGATCTCGGACTGGACATT
  GGTGCCCAGGGAGAAGCCCTTGGATATCGCCAGGACGATCCCAGCTACCGTTCTTTTCACTCTGGTGGAT
  ACGGCCAGGACGCCTTGGGGATGGACCCTATGATGGAGCATGAGATGGGTGGCCACCACCCTGGTGCTGA
  CTATCCAGTTGATGGGCTGCCTGACCTGGGACACGCCCAGGACCTCATGGACGGGCTGCCTCCAGGTGAT
  AGCAATCAGCTGGCCTGGTTTGATACCGACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200930 representing NM_053357
  Red=Cloning site Green=Tags(s)
  
  MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGF
  SQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLI
  NYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVET
  ARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQ
  KMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVC
  SSNKPAIVEAGGMQALGPHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCA
  AGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYG
  LPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQF
  VEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEA
  IEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDI
  GAQGEALGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGD
  SNQLAWFDTDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053357
• ORF Size: 2343 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053357.2, NP_445809.2
• RefSeq Size: 2352 bp
• RefSeq ORF: 2346 bp
• Locus ID: 84353
• UniProt ID: Q9WU82
• Cytogenetics: 8q32
• MW: 85.5 kDa
• Gene Summary: involved in mediating the interaction between cadherins and the actin cytoskeleton [RGD, Feb 2006]