Thra (NM_001017960) Rat Tagged ORF Clone

CAT#: RR200271

Thra (Myc-DDK-tagged ORF) - Rat thyroid hormone receptor alpha (Thra), transcript variant TRalpha1, (10 ug)

Product Images

Circular map for RR200271

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Thra
• Synonyms: c-erbA-1; ERBA1; Thra1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200271 representing NM_001017960
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACAGAAGCCAAGCAAGGTGGAGTGTGGGTCAGACCCAGAGGAGAACAGTGCCAGGTCACCAGATG
  GAAAGCGAAAAAGGAAGAACGGCCAATGTCCCCTGAAAAGCAGCATGTCAGGGTATATCCCTAGTTACCT
  GGACAAAGACGAGCAGTGTGTCGTGTGTGGGGACAAGGCCACCGGTTATCACTACCGCTGTATCACTTGT
  GAGGGCTGCAAGGGCTTCTTTCGCCGTACAATCCAGAAGAACCTCCATCCCACCTATTCCTGCAAATATG
  ACAGCTGCTGCGTCATCGACAAGATCACCCGGAATCAGTGCCAGTTGTGCCGCTTCAAGAAGTGCATCGC
  TGTGGGCATGGCCATGGACCTGGTTCTAGACGATTCAAAGCGGGTGGCCAAACGCAAGCTGATTGAGCAG
  AACCGGGAGAGGAGGCGAAAGGAGGAGATGATCCGCTCGCTGCAGCAACGACCAGAGCCCACTCCTGAAG
  AGTGGGATCTGATCCACGTTGCTACAGAGGCCCATCGCAGCACCAATGCCCAGGGCAGCCATTGGAAACA
  GAGGCGAAAATTCCTGCCGGATGACATTGGCCAGTCACCTATTGTCTCCATGCCGGACGGAGACAAGGTA
  GACCTAGAGGCCTTCAGCGAGTTTACCAAGATCATCACCCCGGCCATCACCCGCGTGGTGGACTTTGCCA
  AAAAACTGCCCATGTTCTCCGAGCTGCCTTGTGAAGACCAGATCATCCTCCTGAAGGGCTGCTGCATGGA
  GATCATGTCCCTGCGGGCAGCTGTCCGCTATGACCCTGAGAGCGACACCCTGACCCTGAGTGGGGAGATG
  GCGGTTAAGCGGGAGCAGCTCAAGAATGGTGGCTTGGGTGTAGTCTCCGACGCCATCTTTGAACTGGGCA
  AGTCACTCTCTGCCTTTAACCTGGATGATACGGAAGTGGCTCTGCTGCAGGCTGTGCTGTTAATGTCAAC
  AGACCGCTCTGGCCTGCTGTGTGTGGACAAGATCGAGAAGAGTCAGGAGGCCTACCTGCTGGCGTTTGAG
  CACTACGTCAACCACCGCAAACACAACATTCCGCACTTCTGGCCCAAGCTGCTGATGAAGGTGACTGACC
  TCCGCATGATCGGGGCCTGCCACGCCAGCCGCTTCCTCCACATGAAAGTCGAGTGCCCCACCGAACTCTT
  CCCCCCACTCTTCCTGGAGGTCTTTGAGGATCAGGAAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200271 representing NM_001017960
  Red=Cloning site Green=Tags(s)
  
  MEQKPSKVECGSDPEENSARSPDGKRKRKNGQCPLKSSMSGYIPSYLDKDEQCVVCGDKATGYHYRCITC
  EGCKGFFRRTIQKNLHPTYSCKYDSCCVIDKITRNQCQLCRFKKCIAVGMAMDLVLDDSKRVAKRKLIEQ
  NRERRRKEEMIRSLQQRPEPTPEEWDLIHVATEAHRSTNAQGSHWKQRRKFLPDDIGQSPIVSMPDGDKV
  DLEAFSEFTKIITPAITRVVDFAKKLPMFSELPCEDQIILLKGCCMEIMSLRAAVRYDPESDTLTLSGEM
  AVKREQLKNGGLGVVSDAIFELGKSLSAFNLDDTEVALLQAVLLMSTDRSGLLCVDKIEKSQEAYLLAFE
  HYVNHRKHNIPHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFEDQEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017960
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017960.1, NP_001017960.1
• RefSeq Size: 2243 bp
• RefSeq ORF: 1233 bp
• Locus ID: 81812
• UniProt ID: P63059
• Cytogenetics: 10q31
• MW: 46.8 kDa
• Gene Summary: binds the promoter of the Na+/H+ exchanger NHE1 and mediates thyroid hormone induced transcriptional activation [RGD, Feb 2006]