Nr1d1 (NM_001113422) Rat Tagged ORF Clone

CAT#: RR200152

Nr1d1 (Myc-DDK-tagged ORF) - Rat nuclear receptor subfamily 1, group D, member 1 (Nr1d1), transcript variant 1, (10 ug)

Product Images

Circular map for RR200152

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nr1d1
• Synonyms: REV-ERBAALPHA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200152 representing NM_001113422
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGACCCTAGACTCCAACAACAACACAGGTGGTGTTATCACCTACATTGGCTCCAGCGGATCCTCCC
  CCAGCCGGACCAGCCCAGAGTCCCTCTATAGTGACAGCTCCAATGGCAGCTTCCAGTCCCTGACTCAAGG
  TTGTCCCACATACTTCCCACCATCACCTACTGGCTCCCTCACCCAGGACCCTGCCCGCTCATTTGGCACT
  GTGCCACCCAGCCTCAGTGATGATAGCTCCCCTTCTTCTGCTTCATCATCATCATCATCTTCCTCCTCCT
  CCTTCTATAATGGGAGCCCCCCCGGAAGTCTACAAGTGGCCATGGAAGACAGCAGCCGAGTGTCCCCCAG
  CAAGGGCACCAGCAACATTACCAAGCTTAACGGCATGGTGCTACTGTGTAAAGTGTGTGGGGACGTGGCC
  TCAGGCTTCCACTATGGCGTGCATGCCTGCGAGGGCTGCAAGGGCTTTTTCCGCCGGAGCATCCAGCAGA
  ACATCCAGTACAAGCGGTGTTTGAAAAACGAGAACTGCTCCATTGTCCGTATCAATCGCAACCGCTGCCA
  GCAGTGTCGCTTCAAGAAGTGTCTCTCCGTTGGCATGTCTAGAGACGCTGTGCGTTTTGGACGTATCCCC
  AAGAGAGAGAAGCAACGAATGCTTGCTGAGATGCAGAACGCCATGAACTTGGCCAACAACCAACTGAGCA
  GCCTGTGCCCTCTAGAGACCTCACCTGCCCCGCACCCCACCTCAGGCTCCGTGGGCCCCTCACCACCTCC
  TGCACCAGCCCCGACACCTTTGGTGGGCTTCTCTCAGTTCCCACAACAGCTGACACCACCCAGATCCCCG
  AGTCCTGAGCCCACCGTGGAGGATGTGATATCCCAGGTGGCCAGGGCCCATCGAGAAATCTTCACCTATG
  CCCATGACAAATTAGGCACCTCACCTGGCAACTTCAATGCCAATCATGCATCAGGTAGCCCTCCGGCTAC
  CACTCCACAGTGCTGGGAGAGTCAGGGATGCCCGTCTACCCCCAACGACAACAACCTTTTGGCGGCTCAG
  CGTCATAATGAAGCACTGAATGGTCTACGCCAGGGCCCCTCCTCCTACCCTCCTACCTGGCCTTCTGGCC
  CTGCCCACCACAGCTGCCACCAGCCTAACAGCAATGGGCATCGCCTGTGCCCCACCCACGTATACTCGGC
  CCCAGAAGGCAAGGCACCTGCCAACGGTCTACGGCAAGGCAACACCAAGAATGTTCTGCTGGCATGTCCC
  ATGAACATGTATCCCCATGGACGTAGTGGCCGGACTGTGCAGGAGATCTGGGAAGACTTCTCTATGAGCT
  TCACACCCGCTGTGCGGGAGGTGGTAGAATTTGCCAAACACATCCCCGGCTTCCGTGACCTTTCTCAGCA
  CGACCAGGTGACCCTGCTTAAGGCTGGCACCTTTGAGGTGCTGATGGTGCGCTTTGCGTCATTGTTCAAC
  GTGAAGGACCAGACAGTCATGTTCCTGAGCCGCACAACCTACAGCCTGCAGGAGCTCGGTGCCATGGGCA
  TGGGTGACCTGCTCAATGCCATGTTTGACTTCAGCGAGAAGCTCAACTCTCTGGCGCTTACTGAGGAGGA
  GCTGGGCCTTTTCACGGCAGTGGTACTTGTCTCTGCAGACCGCTCGGGAATGGAGAATTCCGCTTCGGTG
  GAGCAGCTCCAGGAGACGCTGCTGCGGGCTCTTCGGGCTCTGGTGCTGAAGAACCGGCCCTCGGAGACTT
  CCCGCTTCACCAAACTGCTGCTCAAGCTGCCGGACCTGCGGACCCTGAACAACATGCATTCCGAGAAGCT
  GCTGTCCTTCCGGGTGGACGCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200152 representing NM_001113422
  Red=Cloning site Green=Tags(s)
  
  MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDSSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGT
  VPPSLSDDSSPSSASSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKGTSNITKLNGMVLLCKVCGDVA
  SGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIP
  KREKQRMLAEMQNAMNLANNQLSSLCPLETSPAPHPTSGSVGPSPPPAPAPTPLVGFSQFPQQLTPPRSP
  SPEPTVEDVISQVARAHREIFTYAHDKLGTSPGNFNANHASGSPPATTPQCWESQGCPSTPNDNNLLAAQ
  RHNEALNGLRQGPSSYPPTWPSGPAHHSCHQPNSNGHRLCPTHVYSAPEGKAPANGLRQGNTKNVLLACP
  MNMYPHGRSGRTVQEIWEDFSMSFTPAVREVVEFAKHIPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFN
  VKDQTVMFLSRTTYSLQELGAMGMGDLLNAMFDFSEKLNSLALTEEELGLFTAVVLVSADRSGMENSASV
  EQLQETLLRALRALVLKNRPSETSRFTKLLLKLPDLRTLNNMHSEKLLSFRVDAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113422
• ORF Size: 1845 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113422.1, NP_001106893.1
• RefSeq Size: 2344 bp
• RefSeq ORF: 1848 bp
• Locus ID: 252917
• UniProt ID: Q63503
• Cytogenetics: 10q31
• MW: 66.7 kDa
• Gene Summary: This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (Arntl). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. [provided by RefSeq, Feb 2014]