Dnmt3a (NM_001003958) Rat Tagged ORF Clone

CAT#: RR200037

Dnmt3a (Myc-DDK-tagged ORF) - Rat DNA (cytosine-5-)-methyltransferase 3 alpha (Dnmt3a), transcript variant 1, (10 ug)

Product Images

Circular map for RR200037

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnmt3a
• Synonyms: MGC109195
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200037 representing NM_001003958
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCCAGCGGCCCCGGGGACACCAGCATCTCCTCTCTGGAGCGGGAGGATGATCGAAAGGAAGGAG
  AGGAACAGGAGGAGAACCGTGGTAAGGAAGAGCGTCAGGAGCCCAGCGCCACGGCCCGGAAAGTGGGGAG
  GCCTGGCCGGAAGCGCAAGCACCCACCGGTGGAAAGCAGTGACACCCCCAAGGACCCAGCGGTGACCACC
  AAGTCTCAGCCCACAGCCCAGGACTCTGGGCCCTCAGATCTGCTACCCAATGGAGACTTGGAGAAGCGGA
  GTGAACCCCAACCTGAGGAGGGGAGCCCAGCTGCAGGGCAGAAGGGTGGGGCCCCAGCTGAAGGAGAGGG
  AACTGAGACCCCACCAGAAGCCTCCAGAGCAGTGGAGAATGGCTGCTGCGTAACCAAGGAAGGCCGTGGA
  GCCTCTGCGGGAGAGGGCAAAGAACAGAAGCAGACCAACATCGAATCCATGAAAATGGAGGGCTCCCGGG
  GCCGACTGCGTGGTGGCTTGGGGTGGGAGTCCAGCCTCCGTCAGCGGCCCATGCCAAGACTCACCTTCCA
  GGCAGGGGACCCCTACTACATCAGCAAACGGAAGCGGGATGAGTGGCTGGCACGTTGGAAAAGGGAGGCT
  GAGAAGAAAGCCAAGGTGATTGCAGTAATGAATGCTGTGGAGGAAAGCCAGGCCTCTGGGGAGTCCCAGA
  AGGTGGAGGAGGCCAGCCCTCCTGCAGTGCAGCAGCCCACGGACCCTGCATCTCCCACTGTGGCCACCAC
  CCCTGAGCCAGTAGGGGCTGATGCTGGGGACAAGAATGCTACCAAGGCAGCTGATGACGAGCCCGAGTAT
  GAGGATGGCCGGGGCTTTGGCATTGGAGAGCTGGTGTGGGGGAAACTCCGGGGCTTCTCCTGGTGGCCAG
  GCCGAATTGTGTCTTGGTGGATGACAGGCCGGAGCCGAGCAGCCGAAGGCACTCGCTGGGTCATGTGGTT
  CGGAGATGGCAAATTCTCAGTGGTGTGTGTCGAGAAGCTCATGCCCCTGAGCTCCTTCTGCAGTGCGTTC
  CACCAGGCCACCTACAACAAGCAGCCCATGTACCGCAAAGCCATCTACGAAGTCCTCCAGGTGGCCAGCA
  GCCGTGCAGGGAAGCTGTTCCCAGCATGTCATGACAGCGATGAAAGTGACACTGGCAAGGCTGTGGAGGT
  GCAGAACAAGCAGATGATTGAGTGGGCCCTTGGCGGCTTCCAGCCCTCTGGTCCCAAAGGCCTGGAGCCA
  CCAGAAGAGGAGAAGAATCCTTACAAGGAAGTTTACACCGACATGTGGGTGGAGCCTGAGGCAGCTGCTT
  ATGCCCCACCCCCACCAGCCAAGAAACCCAGAAAGAGCACAACAGAGAAACCCAAGGTCAAGGAGATCAT
  TGATGAACGCACAAGAGAACGGCTCGTGTATGAGGTGCGCCAGAAGTGCCGAAACATCGAGGACATTTGT
  ATCTCATGTGGGAGCCTCAATGTTACCCTGGAGCACCCACTCTTCATTGGTGGAATGTGCCAGAACTGTA
  AGAACTGCTTCTTGGAGTGTGCTTACCAATACGATGACGATGGGTACCAGTCCTACTGTACCATCTGCTG
  TGGGGGGCGCGAAGTGCTCATGTGTGGGAACAACAACTGCTGCAGGTGCTTTTGTGTGGAGTGTGTGGAT
  CTCTTGGTGGGGCCAGGGGCTGCCCAAGCAGCCATTAAGGAAGACCCCTGGAACTGCTACATGTGTGGGC
  ACAAGGGCACGTATGGGCTGCTGCGGAGACGGGAGGACTGGCCTTCTCGACTCCAGATGTTCTTCGCCAA
  TAACCACGACCAGGAATTTGACCCCCCGAAGGTTTACCCACCTGTGCCAGCTGAGAAGAGGAAGCCCATC
  CGGGTGCTATCTCTCTTTGATGGGATTGCTACAGGGCTCCTGGTGCTGAAGGACCTGGGCATCCAAGTGG
  ACCGCTACATCGCCTCCGAGGTGTGCGAGGACTCCATCACAGTGGGCATGGTGCGGCACCAGGGAAAGAT
  CATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGGAGTGGGGCCCATTCGATCTGGTG
  ATTGGGGGCAGTCCCTGCAATGACCTCTCCATCGTCAACCCTGCCCGCAAGGGACTTTACGAGGGCACGG
  GCCGCCTCTTCTTTGAGTTCTACCGCCTCCTGCATGACGCGCGGCCCAAGGAGGGAGATGATCGCCCCTT
  CTTCTGGCTTTTTGAGAATGTGGTGGCCATGGGCGTTAGTGACAAGAGGGACATCTCGAGATTTCTTGAG
  TCTAACCCCGTGATGATCGACGCCAAAGAAGTGTCTGCTGCACACAGGGCCCGTTATTTCTGGGGTAACC
  TTCCTGGCATGAACAGGCCATTGGCATCCACTGTGAATGATAAGCTGGAGTTGCAAGAGTGTCTGGAACA
  CGGCAGAATAGCCAAGTTCAGCAAAGTGAGGACCATTACCACCAGGTCAAACTCCATAAAGCAGGGCAAA
  GACCAGCATTTCCCTGTCTTCATGAATGAGAAGGAAGACATCCTCTGGTGCACTGAAATGGAAAGGGTGT
  TTGGCTTCCCTGTCCACTACACAGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCG
  ATCGTGGAGCGTGCCAGTCATCCGCCACCTCTTCGCTCCGCTGAAGGAATATTTTGCTTGTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200037 representing NM_001003958
  Red=Cloning site Green=Tags(s)
  
  MPSSGPGDTSISSLEREDDRKEGEEQEENRGKEERQEPSATARKVGRPGRKRKHPPVESSDTPKDPAVTT
  KSQPTAQDSGPSDLLPNGDLEKRSEPQPEEGSPAAGQKGGAPAEGEGTETPPEASRAVENGCCVTKEGRG
  ASAGEGKEQKQTNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREA
  EKKAKVIAVMNAVEESQASGESQKVEEASPPAVQQPTDPASPTVATTPEPVGADAGDKNATKAADDEPEY
  EDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAF
  HQATYNKQPMYRKAIYEVLQVASSRAGKLFPACHDSDESDTGKAVEVQNKQMIEWALGGFQPSGPKGLEP
  PEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDIC
  ISCGSLNVTLEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVD
  LLVGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPI
  RVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLV
  IGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLE
  SNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGK
  DQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003958
• ORF Size: 2724 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003958.1, NP_001003958.1
• RefSeq Size: 4173 bp
• RefSeq ORF: 2727 bp
• Locus ID: 444984
• UniProt ID: Q1LZ53
• Cytogenetics: 6q14
• MW: 101.7 kDa
• Gene Summary: member of a family of DNA methyltransferases [RGD, Feb 2006]