Dbf4 (NM_001191748) Rat Untagged Clone

CAT#: RN217080

Dbf4 (untagged) - Rat DBF4 homolog (S. cerevisiae) (Dbf4)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dbf4
• Synonyms: RGD1305854
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217080 representing NM_001191748
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCGAGACCATGAGGATCCACAGCAAAGCACCTCTCCCTGGTGGCATCCAAGACAAAAATGAAA
  AGAATAGACCATCTTTGAAATCCCTGAAAGCTGATAATAGACTGGAAAAATCCAAATATAAGCCGCTTTG
  GGGGAAAATATTTTACCTTGACTTGCCTTCTATCACCATATGTGAAAAACTCCAAAAAGACATAAAGGAA
  CTTGGAGGGCGAGTTGAAGAATTTCTCAGCAAAGATATCAGTTATCTTGTTTCAAATAAAAAGGAAGCAA
  AGTATGCACAAACGTTGGGTCGAGTTTCTCCTGTACCAAGTCCGGAATCTGCATATACTGCGGAAACCAC
  CTCCCCTCATCCCAGCCATGATGGAAGCTCCTTTAAGTCTCAAGACAGAGTGTGTTTGAGCCGAGGAAAG
  TTGTTAGCTGAGAAAGCTGTCAAGGATCATGATTTTATTCCTGCTAATAGTATATTATCAAACGCCTTAT
  CATGGGGAGTAAAGATTCTTCATATTGATGACATTCGCTACTACATTGAACAGAAGAAGAAAGCTTTGGG
  TGCACTCAAGAAACCAAATACTTCTGGGAGGGATTCGGGGAAAAAAGCTGGTACTGGAATACAAAAAGCA
  AGAACAGGGAGACTCAAGAAGCCGTTTTTAAAGGTTGAAGATGTGAACCGGACTTACAAGCCATTTTACC
  TTCAGCTCCCCAGTATGCCTTGTATAAACTACTTCCTTCAGAAGCCTTGCAGTCCGTTTGATATCGATAA
  GTCATCTAGCATGCAGAAACACACTCAGCCTAAACTCAAAATTAATACGGATGGCGATAAATATGGAACC
  CCAGCTCAAGTCCAGTTGAAGGAAAAGAGAAAAAAAGGATATTGTGAATGTTGCTTGCAGAAATATGAAG
  ATCTTGAAACTCACCTTCTGAGTGAGAAACACAGGAACTTTGCACAGAGTAACCAGTATCAAGTTGTTGA
  CGATATTGTATCTAAGTTAATTTTTGATTTTGTGGAATATGAAAGAGACACACCTAAAAAGAAAAGAATA
  AGATACAGTGTTGGATCCCTGTCTTCTGTTTCTGCAAGTGTCCTGCAAAACACTGAACCAAGGGAGAAGC
  TACTGTTGGAGCCCAGTTTCCAGAAAGATGGAGAAGAAAGCAATGGACAACTGCTCGAGCAGAATTTACA
  ATGTGAAGAAACCCAGAAACCTGAAGAAAAGCATGTGTTTGGTTCAGAACCCACAACCTATTCTTCAACT
  GGATTTAAAGGATGTGTTGGGAAACCAGTGAGCCTGATAAATGCAAGTGAGCCTGACTTGAAGCCGGATT
  GTGAACAGCTCCCTCTGCGTGACAATAAGCAGGAGGGCATGCGTGTTGGTTCTGAACATAAAATAACTGT
  AGATAGAAAGGATGTAGACCAGAGAGGAGGCGTCACTCCATGTGTGCCACAGTCATGTACCTATTTCAGT
  ACGGACAGTAATCTACCCCAACCAAAGTTAACTGCAGACATTACACAGTTTTCAGCACAAGATCTGCAAG
  AAAAGGACCTTCATGTGATTGGTCATGATTCTGAGCTGGTGACGTTGAATTCATCAAGAGAGCAGCTAAC
  AGTGAAGGCCAGACCTCCACCCTGTAGTCCTCAGGAGCCTCATGAGGGTCACAGTGAGCATATGGAGAGT
  CTGCCTTGTGGTAAAATCCAGCGGAAAGTGAGAATGTTATTAGGGCAAAAGAAAGCAAATGTGGAACCTG
  GTGCCGAATGGGATAAGAACAGAACTGAGTATCTTCCTGCACATGGAGACAGAACTTGTGGGTCACCAGT
  ACAGTCTCTGTTGGACCTTTTTCAGACAAGCGGGGAGAAATCAGAATTTTTGGGTTTTACAAGCTACACT
  GAAAACAGTGGAATCTGTGATGTTTTAGATATTTGGGAGGAAGACAATTCAAGTAGCCTGTTGTCAACAT
  TTTTTTCTTCCCCTTCAACTTCTGCATTTGTTGGATTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001191748
• Insert Size: 2001 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191748.1, NP_001178677.1
• RefSeq Size: 2263 bp
• RefSeq ORF: 2001 bp
• Locus ID: 312046
• Cytogenetics: 4q12