Ache (NM_172009) Rat Untagged Clone

CAT#: RN212424

Ache (untagged ORF) - Rat acetylcholinesterase (Ache), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ache
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212424 representing NM_172009
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCCTCCCTGGTATCCCCTGCATACACCCTCCCTGGCTTCTCCACTCCTCTTCCTCCTCCTCTCCC
  TCCTGGGAGGAGGGGCAAGGGCTGAGGGCCGGGAAGACCCTCAGCTGCTGGTGAGGGTTCGAGGGGGCCA
  GCTGAGGGGCATCCGCCTGAAGGCCCCTGGAGGCCCAGTCTCAGCTTTTCTGGGCATCCCCTTTGCAGAG
  CCACCTGTGGGCTCACGTAGATTTATGCCACCAGAGCCCAAGCGCCCCTGGTCAGGAATATTGGATGCTA
  CCACCTTCCAAAATGTCTGCTACCAATACGTGGACACCCTGTACCCTGGGTTTGAGGGTACCGAGATGTG
  GAACCCCAATCGAGAGCTGAGTGAAGACTGCCTTTATCTTAATGTGTGGACACCATACCCCAGGCCTACT
  TCTCCCACACCTGTCCTCATCTGGATCTATGGGGGTGGTTTCTACAGTGGAGCATCCTCCTTGGACGTGT
  ATGACGGCCGTTTCCTGGCCCAGGTTGAGGGAACCGTGTTGGTATCTATGAACTACCGAGTGGGAACCTT
  TGGCTTCTTGGCTCTACCAGGAAGCAGAGAAGCCCCTGGCAATGTAGGCCTGCTGGATCAACGGCTTGCC
  TTGCAATGGGTACAAGAAAATATCGCAGCCTTTGGGGGAGACCCAATGTCAGTGACTCTGTTTGGGGAGA
  GTGCAGGTGCAGCCTCAGTGGGCATGCACATTCTGTCCCTGCCCAGCAGGAGCCTCTTCCACAGGGCTGT
  CCTGCAGAGTGGCACACCCAATGGGCCCTGGGCCACTGTGAGTGCGGGAGAGGCCAGGCGCAGGGCCACA
  CTGCTGGCCCGCCTTGTGGGCTGTCCCCCAGGTGGCGCTGGTGGCAATGACACCGAGCTGATATCCTGCT
  TGAGGACAAGGCCCGCTCAGGACCTGGTGGACCACGAGTGGCATGTGCTGCCTCAAGAAAGTATCTTCCG
  GTTTTCCTTCGTGCCTGTGGTGGACGGGGATTTCCTCAGTGACACGCCGGACGCTCTCATCAATACTGGA
  GATTTTCAAGACCTGCAGGTGCTGGTGGGTGTGGTGAAGGACGAGGGCTCCTACTTTCTGGTTTACGGGG
  TCCCAGGCTTCAGCAAAGACAATGAATCTCTCATCAGCCGGGCCCAGTTCCTGGCTGGGGTGCGGATCGG
  TGTACCCCAAGCGAGTGACCTGGCGGCCGAGGCTGTGGTCCTGCATTATACAGACTGGCTGCACCCTGAG
  GACCCTGCCCACCTGAGAGATGCCATGAGTGCGGTGGTAGGCGACCACAACGTTGTGTGCCCTGTGGCCC
  AGCTGGCTGGGCGACTGGCTGCCCAAGGGGCTCGGGTCTATGCCTACATCTTTGAACACCGTGCCTCCAC
  ATTGACTTGGCCCCTCTGGATGGGGGTGCCCCATGGCTATGAAATCGAGTTCATCTTTGGGCTCCCCCTG
  GATCCCTCACTGAACTACACCGTGGAGGAGAGAATCTTTGCTCAGCGACTTATGCAATACTGGACCAATT
  TTGCCCGCACAGGGGACCCCAATGACCCTCGAGACTCTAAGTCTCCACGGTGGCCACCGTACACCACTGC
  CGCGCAGCAATACGTGAGCCTGAACCTGAAGCCTTTGGAGGTGCGGCGGGGACTGCGCGCCCAGACCTGC
  GCCTTCTGGAATCGTTTTCTCCCCAAATTGCTCAGCGCCACAGACACGCTGGACGAGGCGGAGCGCCAGT
  GGAAGGCCGAGTTCCACCGCTGGAGCTCCTACATGGTGCACTGGAAGAACCAGTTCGACCACTATAGCAA
  GCAGGAACGCTGCTCAGACCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172009
• Insert Size: 1845 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172009.1, NP_742006.1
• RefSeq Size: 2066 bp
• RefSeq ORF: 1845 bp
• Locus ID: 83817
• UniProt ID: P37136
• Cytogenetics: 12q12
• Gene Summary: catalyzes the hydrolysis of acetylcholine to choline and acetate; plays a role in neuronal development and synapse formation [RGD, Feb 2006]