Actn1 (NM_031005) Rat Untagged Clone

CAT#: RN204782

Actn1 (untagged ORF) - Rat actinin, alpha 1 (Actn1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Actn1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204782 representing NM_031005
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCATTATGATTCCCAGCAGACCAATGACTATATGCAGCCTGAAGAGGACTGGGACCGGGACCTGC
  TCCTGGATCCGGCCTGGGAGAAGCAGCAGAGAAAGACCTTCACGGCCTGGTGCAACTCCCACCTGCGCAA
  AGCAGGGACCCAGATTGAGAACATTGAAGAGGACTTCCGAGATGGCCTGAAGCTCATGCTGCTCCTGGAG
  GTCATCTCAGGTGAGCGTTTGGCCAAGCCAGAGAGAGGCAAGATGCGAGTGCACAAGATCTCCAACGTCA
  ACAAGGCCCTGGATTTCATAGCCAGCAAAGGGGTCAAGCTGGTGTCCATTGGAGCTGAAGAAATTGTGGA
  CGGGAATGTGAAGATGACCCTGGGCATGATCTGGACCATCATCCTTCGTTTTGCCATCCAAGACATCTCT
  GTGGAAGAGACATCAGCTAAAGAAGGGTTGCTCCTGTGGTGTCAGAGGAAGACAGCCCCATATAAAAATG
  TCAACATCCAAAACTTCCACATCAGTTGGAAGGATGGTCTTGGTTTCTGTGCCTTGATCCACCGGCACCG
  GCCCGAGTTGATTGACTATGGAAAGCTTCGAAAGGATGATCCACTCACAAACCTGAACACAGCCTTTGAT
  GTGGCAGAGAGATACCTGGACATTCCAAAGATGCTGGATGCAGAAGACATCGTCGGAACTGCCCGGCCAG
  ATGAGAAAGCCATTATGACCTATGTGTCTAGCTTCTACCATGCCTTCTCTGGAGCCCAGAAGGCGGAAAC
  AGCAGCCAATCGCATCTGTAAAGTGTTGGCCGTCAATCAAGAGAATGAACAGCTTATGGAAGACTATGAG
  AAGCTGGCCAGTGATCTGTTAGAGTGGATCCGCCGCACCATCCCATGGCTGGAGAATCGGGTGCCCGAGA
  ACACCATGCAGGCCATGCAGCAGAAGCTGGAGGACTTCCGAGACTACCGACGCCTGCACAAGCCGCCCAA
  GGTGCAGGAGAAGTGCCAGCTGGAGATCAACTTCAACACACTGCAGACCAAGCTGCGGCTAAGCAACCGG
  CCTGCCTTCATGCCCTCCGAGGGCAGGATGGTCTCGGATATCAACAATGCCTGGGGCTGCCTGGAACAGG
  CAGAGAAGGGCTACGAGGAGTGGTTGCTGAATGAGATCCGGAGGCTAGAGAGGTTGGACCACCTGGCAGA
  GAAGTTCCGGCAGAAGGCCTCCATCCATGAAGCCTGGACAGACGGCAAAGAAGCCATGCTGCGGCAGAAG
  GATTATGAGACGGCCACCTTGTCCGAGATCAAGGCCCTACTCAAGAAGCACGAGGCCTTTGAGAGTGACC
  TGGCTGCCCACCAGGACCGCGTGGAGCAGATCGCGGCCATTGCCCAGGAGCTTAATGAGCTGGACTATTA
  TGACTCACCAAGTGTCAACGCTCGATGCCAAAAGATCTGTGACCAGTGGGACAATCTAGGGGCCCTGACT
  CAGAAGCGGAGGGAAGCTTTAGAGCGAACAGAGAAGCTGCTGGAGACCATCGACCAGCTATACTTGGAGT
  ACGCCAAGAGGGCTGCACCCTTCAACAACTGGATGGAGGGAGCCATGGAGGACCTTCAAGACACCTTCAT
  CGTACATACCATTGAGGAGATCCAGGGACTGACCACAGCCCATGAGCAGTTCAAGGCTACCCTCCCGGAT
  GCAGACAAGGAGCGCCTGGCCATCCTGGGCATCCACAATGAAGTGTCCAAGATTGTCCAGACCTATCATG
  TCAACATGGCAGGCACCAACCCCTATACAACCATCACACCTCAGGAGATCAATGGCAAATGGGACCATGT
  ACGGCAGCTGGTGCCCCGGAGGGACCAGGCTCTAACAGAGGAGCACTCGAGGCAGCAGCACAACGAGAGG
  TTACGCAAGCAGTTTGGGGCACAAGCCAATGTCATCGGACCCTGGATCCAGACCAAGATGGAGGAGATTG
  GGAGGATCTCCATTGAGATGCACGGTACCCTGGAGGACCAACTCAGCCACCTGCGGCAGTATGAAAAGAG
  CATCGTCAATTATAAGCCAAAGATTGACCAGCTGGAGGGCGACCACCAGCTCATCCAGGAAGCACTTATC
  TTCGACAATAAGCACACCAACTACACAATGGAGCACATCCGTGTGGGCTGGGAGCAGCTGCTCACCACCA
  TTGCCAGGACCATCAATGAAGTGGAGAACCAGATCTTGACCCGGGATGCCAAAGGCATCAGCCAGGAACA
  GATGAACGAATTCCGGGCCTCCTTCAACCACTTTGACCGGGATCACTCCGGCACGTTGGGTCCCGAAGAG
  TTCAAAGCCTGCCTCATCAGCTTGGGTTATGATATTGGCAACGACCCCCAGGGAGAGGCAGAATTTGCCC
  GGATCATGAGCATTGTAGACCCCAACCGCTTGGGGGTAGTGACATTCCAGGCCTTCATCGACTTCATGTC
  CCGAGAGACGGCTGACACAGATACAGCGGATCAGGTCATGGCTTCCTTCAAGATCCTGGCTGGAGATAAG
  AATTACATCACCGGGGATGAGCTGCGCCGCGAGCTGCCACCTGACCAAGCCGAGTACTGCATCGCGAGAA
  TGGCCCCCTACGCGGGCCCTGACTCCGTGCCGGGTGCTCTAGACTACATGTCCTTCTCCACGGCGCTGTA
  CGGCGAGAGTGACCTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031005
• Insert Size: 2679 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031005.3, NP_112267.1
• RefSeq Size: 2956 bp
• RefSeq ORF: 2679 bp
• Locus ID: 81634
• UniProt ID: Q9Z1P2
• Cytogenetics: 6q24
• Gene Summary: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (By similarity).[UniProtKB/Swiss-Prot Function]