App (NM_019288) Rat Untagged Clone

CAT#: RN204173

App (untagged ORF) - Rat amyloid beta (A4) precursor protein (App), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: App
• Synonyms: Abeta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204173 representing NM_019288
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCAGCCTGGCACTGCTCCTGCTGGCCGCCTGGACGGTTCGGGCTCTGGAGGTGCCCACTGATG
  GCAATGCCGGTCTGCTGGCAGAACCCCAGATCGCCATGTTCTGTGGTAAACTCAACATGCACATGAATGT
  GCAGAATGGAAAATGGGAGTCAGACCCATCAGGGACCAAAACCTGCATTGGCACCAAGGAGGGAATCCTG
  CAGTACTGCCAAGAGGTCTACCCTGAACTGCAGATCACAAACGTGGTGGAAGCCAACCAGCCAGTGACCA
  TCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACGCACACCCACATCGTGATTCCTTACCGGTG
  CCTAGTTGGTGAGTTTGTAAGCGATGCCCTTCTCGTGCCCGACAAGTGCAAGTTTCTACACCAGGAGCGG
  ATGGACGTTTGTGAGACCCATCTTCACTGGCATACTGTTGCCAAAGAGACATGCAGTGAGAAGAGCACTA
  ACTTGCACGACTATGGCATGCTGCTGCCCTGTGGCATCGACAAGTTCCGAGGGGTCGAGTTCGTGTGCTG
  CCCGTTGGCGGAGGAGAGCGACAGCATCGATTCTGCGGACGCAGAGGAGGACGACTCCGATGTCTGGTGG
  GGTGGAGCGGACACAGACTATGCTGATGGCGGTGAAGACAAAGTCGTAGAAGTAGCCGAAGAGGAGGAAG
  TGGCCGATGTTGAGGAAGAAGAAGCTGAGGATGACGAGGATGTGGAGGATGGGGATGAGGTGGAGGAGGA
  GGCCGAGGAGCCCTACGAAGAGGCCACAGAGAGAACAACCAGCATTGCCACCACTACCACAACTACCACT
  GAGTCTGTGGAGGAGGTAGTCCGAGAGGTGTGCTCTGAACAAGCTGAGACCGGGCCATGCCGTGCAATGA
  TCTCCCGCTGGTACTTTGATGTCACTGAAGGAAAGTGCGCCCCATTCTTTTACGGCGGATGTGGCGGCAA
  CAGGAACAACTTTGACACTGAAGAGTACTGCATGGCGGTGTGTGGCAGCGTGTCATCCCAAAGTTTACTC
  AAGACTACCAGTGAACCTCTTCCCCAAGATCCGGTTAAACTTCCCACGACGGCAGCCAGCACCCCTGACG
  CAGTCGACAAGTACCTGGAGACCCCCGGAGATGAGAACGAGCACGCCCATTTCCAGAAAGCCAAAGAGAG
  GTTGGAAGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAGGAGGCAGAACGTCAAGCC
  AAGAATTTGCCCAAAGCTGACAAGAAGGCCGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTCTGGAAC
  AGGAAGCAGCCAACGAGAGGCAGCAGCTTGTAGAGACACACATGGCCAGAGTTGAAGCCATGCTCAATGA
  TCGCCGTCGCCTGGCCCTCGAGAATTACATCACCGCACTGCAGGCGGTGCCTCCAAGGCCTCATCATGTG
  TTCAACATGCTGAAGAAGTACGTCCGTGCAGAGCAGAAGGACAGACAGCACACCCTAAAGCATTTTGAAC
  ATGTGCGCATGGTGGACCCCAAGAAAGCTGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGAT
  CTACGAGCGCATGAACCAGTCTCTGTCCCTGCTCTACAACGTCCCTGCCGTGGCTGAGGAGATTCAAGAT
  GAAGTTGACGAGCTGCTTCAGAAGGAGCAGAACTACTCCGACGACGTCTTAGCCAACATGATCAGTGAAC
  CCAGAATCAGTTACGGCAACGATGCTCTCATGCCTTCTTTGACTGAAACGAAGACCACTGTGGAGCTCCT
  TCCCGTGAATGGCGAATTCAGCCTGGATGATCTCCAACCGTGGCATCCTTTTGGGGTGGACTCTGTGCCA
  GCCAATACAGAAAATGAAGTTGAGCCTGTCGACGCCCGCCCCGCTGCTGACCGAGGACTGACCACTCGAC
  CAGGGTCTGGGTTGACAAACATCAAGACAGAAGAGATCTCAGAAGTGAAGATGGATGCGGAGTTCGGACA
  TGATTCAGGCTTCGAAGTCCGCCATCAAAAACTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGT
  GCCATCATTGGACTCATGGTGGGTGGCGTTGTCATAGCAACAGTGATTGTCATCACCTTGGTGATGCTGA
  AGAAGAAACAGTACACATCCATCCATCATGGCGTGGTGGAGGTTGACGCTGCTGTGACCCCGGAGGAGCG
  CCACCTCTCCAAGATGCAGCAGAATGGATATGAGAATCCAACATACAAGTTCTTTGAGCAGATGCAGAAC
  TAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019288
• Insert Size: 2313 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019288.1, NP_062161.1
• RefSeq Size: 2340 bp
• RefSeq ORF: 2313 bp
• Locus ID: 54226
• UniProt ID: P08592
• Cytogenetics: 11q11
• Gene Summary: activates voltage dependent calcium channels; may induce neuronal apoptosis [RGD, Feb 2006]