Shmt1 (NM_001047842) Rat Untagged Clone

CAT#: RN203948

Shmt1 (untagged ORF) - Rat serine hydroxymethyltransferase 1 (soluble) (Shmt1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Shmt1
• Synonyms: LRRGT00032; mShmt; Shmt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN203948 representing NM_001047842
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGACTGTGGAGGCATGGGTGCAGATATTCCTGCTTCCAGTTGAGAAGTCCGATCCTGTGTCGA
  GGCTGGACCCTGACAGGATGGGACATGTGAAAGGGCCAGTTCAGATGATCCAGAACTGCAGAATCTCCAC
  GACACAGGGCAACAACAGGATAACCCAGAGGAGTCCTGGATACACTCCAGATAGTCCTACAAAGTCCTTC
  TCACTGTGGAACAGCCAAAATCTGCTAGTTCAGTCCATGAAACTGGCTGCCTCAGCAGTCTCAATCTGGT
  TCTGGGGTTCTTGGAGGATTCCTAGAGAGCTGCTGGTTTTCAGTTTACATTGGAATCTTAAGGAAGTTGG
  ACTTAGCACCAGCCCCAGCAACAGCACAAACTTGCCAGCAAGAGTAAAAGGCAAGCAGGCAGAAAAGCAA
  AGCTTCCTTCTTCTAAGTTCTTTTATCTGGGTTGCCATCAGAAGCTACAGCTCCTTTTGTCAGGTTTTGC
  CCAAGTTTCTGCTCCGAGCTCGGGCGAATGGCGCCTGGGCGGCTGAGGGCGGGCTCTACCTGGACCCGCC
  CACTGATTGGGCCGCTCTAGGGGTGGGGCGGGGCCAGCCGCGCTGTGCAATGGCAGAGAGGGATGCCACC
  GTGTGGGCCTCTCATGAGAAGATGTTGACACAGCCCCTGAAGGAGAGCGATGCTGAGGTTTACAGCATCA
  TTAAAAAGGAGAGCAACCGGCAGAGGGTCGGATTGGAGCTGATTGCCTCGGAGAACTTTGCCAGCAGAGC
  TGTGTTGGAGGCCCTAGGATCTTGCTTAAATAACAAATACTCTGAGGGGTACCCAGGCCAAAGGTATTAT
  GGTGGGACTGAGTTCATCGATGAGCTGGAGACGCTGTGTCAGAAGCGTGCATTACAGGCCTACCATCTGG
  ATCCTCAGTGCTGGGGCGTCAATGTCCAGCCTTATTCAGGCTCCCCTGCAAACTTTGCTGTGTATACTGC
  CCTAGTAGAGCCCCATGGGCGAATCATGGGTTTGGACCTGCCAGATGGTGGCCATCTGACTCATGGCTTC
  ATGACAGACAAGAAGAAAATCTCTGCCACCTCTATCTTCTTTGAATCCATGCCTTATAAGGTATACCCAG
  ACACTGGGTACATCAACTATGACCAGCTAGAGGAGAATGCCAGCCTCTTCCACCCGAAGCTGATCATTGC
  AGGGACCAGCTGCTACTCCCGGAACCTGGACTATGCGCGGCTGCGAAAGATTGCGGATGATAATGGGGCA
  TATCTCATGGCAGACATGGCACACATCAGTGGGCTCGTGGCAGCTGGTGTGGTGCCCTCACCCTTTGAAC
  ACTGTCATGTAGTAACCACTACAACCCACAAGACCCTGAGAGGCTGCCGTGCTGGTATGATATTCTACAG
  AAAGGGTGTGCGCAGTGTTGATCCCAAGACTGGCGAAGAGACTTACTATGAACTGGAGTCCCTCATCAAC
  TCGGCCGTGTTCCCAGGCCTGCAGGGCGGCCCTCACAACCATGCCATTGCTGGTGTTGCTGTGGCCCTGA
  AGCAAGCCATGACTACAGAATTCAAAATCTACCAGCTCCAGGTGTTGGCCAACTGCAGGGCTCTGTCTGA
  TGCACTCACAGAGCTAGGCTACAAAATAGTCACAGGTGGCTCTGATAATCACTTGATCCTAATGGATCTG
  CGTCCTAAGGGTACTGATGGCGGAAGGGCTGAGAAGGTACTGGAAGCCTGTTCTATCGCCTGCAACAAGA
  ATACCTGCCCAGGTGACAAGAGTGCGTTACGGCCCAGTGGCCTGCGACTGGGAACTCCAGCATTGACATC
  CCGAGGACTTTTGGAAGAAGACTTCCAAAAAATAGCACACTTTATCCACAGAGGGATAGAGTTGACTCTG
  CAGATTCAGAGCCACATGACCATGAGGGCCACACTGAAAGAGTTCAAGGAGAAGCTGACAGGGGATGAGA
  AGTTCCAGAGTGCTGTGGCAGCTCTCCGGGAAGAAGTGGAGAATTTTGCTTCCAACTTCTCACTACCTGG
  ACTTCCTGACTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001047842
• Insert Size: 2046 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001047842.1, NP_001041307.1
• RefSeq Size: 2046 bp
• RefSeq ORF: 2046 bp
• Locus ID: 287379
• Cytogenetics: 10q22