Azin2 (NM_001014261) Rat Untagged Clone

CAT#: RN202365

Adc (untagged ORF) - Rat similar to ornithine decarboxylase-like protein (LOC366473), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Azin2
• Synonyms: Adc; AzI2; ODC-p; RGD1564776
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202365 representing NM_001014261
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCTACCTCAGTGAATCGGACTTTGTGATGGTGGAGGAGGGCTTCAGCACCCGGGATCTGCTGG
  AGGAACTCACTCTGGGGGCCTCCCAGGCCACCACGGGCAAGGTGGCTGCCTTCTTCGTGGCCGACCTGGG
  TGCTGTAGTGAGGAAGCACTTCTGCTTTCTGAAGTACCTGCCTCGAGTCCGGCCTTTTTATGCTGTCAGG
  TGCAACAGCAGTCTTGGCGTGCTGAAGGTCCTGGCCGAGCTGGGACTGGGCTTCAGCTGTGCCAGCAAGG
  CAGAGATGGAGTTGGTCCAGCACATTGGTGTCCCTGCCAGTAAGATCATCTGTGCCAACCCCTGTAAGCA
  AGTTGCCCAGATCAAGTATGCTGCCAAGCACGGGGTGAGACTGCTAAGCTTCGACAATGAAGTGGAACTG
  GCCAAGGTGGTCAAGAGCCACCCCAGTGCCAAGATGGTTCTGTGCATTGCTACCCAGGACTCCCACTCTC
  TGAATCACCTGAGCTTGAGGTTCGGGGCGTCGCTGAAATCCTGCAGACATCTGCTCGAGAACGCCAAGCA
  GAGCCATGTGGAGGTGGTGGGTGTGAGTTTTCACATTGGTAGTGGCTGTCCTGACCCTCAGGCCTATGCC
  CAGTCCATCGCGGATGCCAGGCTCGTGTTTCAAATGGGTGCGGAGCTGGGCCACACGATGAACATCCTGG
  ACCTTGGCGGCGGCTTTCCTGGCTTAGAGGGAGCCAAAGTGAGATTTGAAGAGATGGCCTCAGTGATTAA
  CTCAGCCTTGGACCTGTACTTCCCTGAGGGCTGCGGTGTGGACATCCTTGCTGAGCTGGGCCGCTACTAT
  GTGACGTCAGCCTTCACTGTGGCCGTCAGCATCGTTGCCAAGAAGGAGGTTCTGGACCAGCCCAGCAGGG
  AGGAGCAAACCGGCGCAGCCCCTAAGAGCATCGTGTACCACCTTGATGAAGGTGTTTATGGGGTCTTCAA
  CTCAGTCCTGTTTGACAACACCTGCCCCACCCCCGCCCTGCAGAAGAAACCATCTGCGGATCAACCGCTG
  TACAGCAGTAGCCTGTGGGGCCCAGCAGTTGACGGCTGCGACTGTGTGGCTGAGGGCCTATGGCTGCCGC
  AACTACAAGTAGGGGACTGGCTGGTCTTTGACAACATGGGTGCTTACACCGTGGACACAAAGTCCCTTCT
  CGGGGGGACCCAGGCCTGCAGAGTCACTTATGCCATGTCCCGGCTAGCCTGGGAAGCCCTTCAAGGGCAG
  CTGCTGCCTGCAGAAGAAGACCAGGACGCCGAGGGTGTGTGCAAACCTCTGTCCTGCGGCTGGGAGATCA
  CAGACAGCCTGTGTGTGGGCCCTGTCTTCACCCCAGCAAGCATCATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001014261
• Insert Size: 1380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014261.3, NP_001014283.2
• RefSeq Size: 1955 bp
• RefSeq ORF: 1380 bp
• Locus ID: 366473
• Cytogenetics: 5q36
• Gene Summary: The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 2, the second member of this gene family. Like antizyme inhibitor 1, antizyme inhibitor 2 interacts with all 3 antizymes and stimulates ODC activity and polyamine uptake. However, unlike antizyme inhibitor 1, which is ubiquitously expressed and localized in the nucleus and cytoplasm, antizyme inhibitor 2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Recent studies indicate that antizyme inhibitor 2 is also expressed in specific cell types in ovaries, adrenal glands and pancreas, and in mast cells. The exact function of this gene is not known, however, available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. There has been confusion in literature and databases over the nomenclature of this gene, stemming from an earlier report that a human cDNA clone (identical to ODCp/AZIN2) had arginine decarboxylase (ADC) activity (PMID:14738999). Subsequent studies in human and mouse showed that antizyme inhibitor 2 was devoid of arginine decarboxylase activity (PMID:19956990). [provided by RefSeq, Sep 2014]