Tmem147 (NM_001038494) Rat Untagged Clone

CAT#: RN200612

Tmem147 (untagged ORF) - Rat transmembrane protein 147 (Tmem147), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Tmem147
• Synonyms: RGD1304706
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200612 representing NM_001038494
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTGTTCCACTTCGGGAACTGCTTCGCCCTCGCCTACTTTCCCTATTTCATCACGTACAAATGCA
  GCGGCCTGTCTGAGTACAACGCCTTCTGGAAATGCGTCCAGGCCGGGGTCACCTACCTCTTTGTGCAGCT
  ATGTAAGATGTTGTTCTTGGCCACTTTCTTCCCCACCTGGGAAGGGGGCATCTATGACTTCATTGGGGAA
  TTCATGAAGGCCAGTGTGGATGTGGCAGACCTGATAGGCCTAAACCTTGTCATGTCCCGGAATGCAGGCA
  AGGGGGAGTACAAGATCATGGTTGCTGCCCTGGGCTGGGCCACTGCTGAACTCATTATGTCCCGCTGCAT
  CCCTCTCTGGGTGGGAGCCCGAGGCATTGAATTTGACTGGAAATATATCCAGATGAGCATTGACTCCAAC
  ATCAGTCTGGTCCACTACATTGTTGCATCAGCTCAGGTCTGGATGATAACACGCTATGACCTGTACCACA
  CCTTCCGGCCTGCTGTTCTTCTCCTAATGTTCCTTAGTGTCTACAAGGCCTTTGTCATGGAGACCTTTGT
  CCATCTCTGCTCCTTGGGTAGTTGGACAGCGCTGCTGGCCCGGGCAGTGGTGACAGGATTGCTGGCCCTC
  AGCACCTTGGCCCTGTATGTTGCTGTTGTCAATGTACACTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001038494
• Insert Size: 675 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038494.1, NP_001033583.1
• RefSeq Size: 881 bp
• RefSeq ORF: 675 bp
• Locus ID: 292792
• UniProt ID: Q2TA63
• Cytogenetics: 1q21
• Gene Summary: Acts as a negative regulator of CHRM3 function, most likely by interfering with its trafficking to the cell membrane (By similarity). Negatively regulates CHRM3-mediated calcium mobilization and activation of RPS6KA1/p90RSK activity (By similarity).[UniProtKB/Swiss-Prot Function]