Cox6a2 (NM_012812) Rat Untagged Clone

CAT#: RN200438

Cox6a2 (untagged ORF) - Rat cytochrome c oxidase, subunit VIa, polypeptide 2 (Cox6a2), nuclear gene encoding mitochondrial protein, transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cox6a2
• Synonyms: COX6AH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200438 representing NM_012812
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGCCTCTTAAGGTCCTCAGTCGGAGCATGGCCAGCGCATCCAAAGGAGACCACGGAGGCGCAG
  GAGCCAACACTTGGCGCCTCCTGACCTTTGTGCTGGCTCTCCCCAGCGTAGCTCTCTGCTCGCTTAACTG
  CTGGATGCACGCTGGCCACCATGAGCGCCCAGAATTCATCCCCTATCACCACCTCCGCATCCGAACCAAG
  CCCTTCTCCTGGGGGGATGGGAACCACACGCTTTTCCACAATCCCCACGTCAATCCTTTGCCCACCGGCT
  ACGAGCAGCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012812
• Insert Size: 294 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012812.3, NP_036944.2
• RefSeq Size: 493 bp
• RefSeq ORF: 294 bp
• Locus ID: 25278
• Cytogenetics: 1q37
• Gene Summary: heart isoform of subunit VIa of cytochrome c oxidase [RGD, Feb 2006]
  Transcript Variant: This variant (1) encodes the longer transcript.