PER3 (NM_001289861) Human Tagged ORF Clone

CAT#: RG240035

PER3 (tGFP-tagged) - Human period circadian clock 3 (PER3), transcript variant 2

Product Images

Circular map for RG240035

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PER3
• Synonyms: FASPS3; GIG13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240035 representing NM_001289861.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCCCGCGGGGAAGCTCCTGGCCCCGGGAGACGGGGGGCTAAGGACGAGGCCCTGGGCGAAGAATCG
  GGGGAGCGGTGGAGCCCCGAGTTCCATCTGCAGAGGAAATTGGCGGACAGCAGCCACAGTGAACAGCAA
  GATCGAAACAGAGTTTCTGAAGAACTTATCATGGTTGTCCAAGAAATGAAAAAATACTTCCCCTCGGAG
  AGACGCAATAAACCAAGCACTCTAGATGCCCTCAACTATGCTCTCCGCTGTGTCCACAGCGTTCAAGCA
  AACAGTGAGTTTTTCCAGATTCTCAGTCAGAATGGAGCACCTCAGGCAGATGTGAGCATGTACAGTCTT
  GAGGAGCTGGCCACTATCGCTTCAGAACACACTTCCAAAAACACAGATACCTTTGTGGCAGTATTTTCA
  TTTCTGTCTGGAAGGTTAGTGCACATTTCTGAACAGGCTGCTTTGATCCTGAATCGTAAGAAAGATGTC
  CTGGCGTCTTCTCACTTTGTTGACCTGCTTGCACCTCAAGACATGAGGGTATTCTACGCGCACACTGCC
  AGAGCTCAGCTTCCTTTCTGGAACAACTGGACCCAAAGAGCAGCTGCACGGTATGAATGTGCTCCGGTG
  AAACCTTTTTTCTGCAGGATCCGTGGAGGTGAAGACAGAAAGCAAGAGAAGTGTCACTCCCCATTCCGG
  ATCATCCCCTATCTGATTCATGTACATCACCCTGCCCAGCCAGAATTGGAATCGGAACCTTGCTGTCTC
  ACTGTGGTTGAAAAGATTCACTCTGGTTATGAAGCTCCTCGGATCCCAGTGAATAAAAGAATCTTCACC
  ACCACACACACCCCAGGGTGTGTTTTTCTTGAAGTAGATGAAAAAGCAGTGCCTTTGCTGGGTTACCTA
  CCTCAGGACCTGATTGGAACATCGATCCTAAGCTACCTGCACCCTGAAGATCGTTCTCTGATGGTTGCC
  ATACACCAAAAAGTTTTGAAGTATGCAGGGCATCCTCCCTTTGAACATTCTCCCATTCGATTTTGTACT
  CAAAACGGAGACTACATCATACTGGATTCCAGTTGGTCCAGCTTTGTGAATCCCTGGAGCCGGAAGATT
  TCTTTCATCATTGGTCGGCATAAAGTTCGAACGAGCCCACTAAATGAGGATGTTTTTGCTACCAAAATT
  AAAAAGATGAACGATAATGACAAAGACATAACAGAATTACAAGAACAAATTTACAAACTTCTCTTACAG
  CCAGTTCACGTGAGCGTGTCCAGCGGCTACGGGAGCCTGGGGAGCAGCGGGTCGCAGGAGCAGCTTGTC
  AGCATCGCCTCCTCCAGTGAGGCCAGTGGGCACCGTGTGGAGGAGACGAAGGCGGAGCAGATGACCTTG
  CAGCAGGTCTATGCCAGTGTGAACAAAATTAAAAATCTGGGTCAGCAGCTCTACATTGAGTCAATGACC
  AAATCATCATTCAAGCCAGTGACGGGGACACGCACAGAACCGAATGGTGGTGGTGAGTCAGCGAATGGT
  GGTGGTGAATGTAAGACCTTTACTTCCTTCCACCAAACACTGAAAAACAATAGTGTGTACACTGAGCCC
  TGTGAGGATTTGAGGAACGATGAGCACAGCCCATCCTATCAACAGATCAACTGTATCGACAGTGTCATC
  AGATACCTGAAGAGCTACAACATTCCAGCTTTGAAAAGAAAGTGTATCTCCTGTACAAATACAACTTCT
  TCCTCCTCAGAAGAAGACAAACAGAACCACAAGGCAGATGATGTCCAAGCCTTACAAGCTGGTTTGCAA
  ATCCCAGCCATACCTAAATCAGAAATGCCAACAAATGGACGGTCCATAGACACAGGAGGAGGAGCTCCA
  CAGATCCTGTCCACGGCGATGCTGAGCTTGGGGTCGGGCATAAGCCAATGCGGTTACAGCAGCACCATT
  GTCCATGTCCCACCCCCAGAGACAGCCAGGGATGCTACCCTCTTCTGTGAGCCCTGGACCCTGAACATG
  CAGCCAGCCCCTTTGACCTCGGAAGAATTTAAACACGTGGGGCTCACAGCGGCTGTTCTGTCAGCGCAC
  ACCCAGAAGGAAGAGCAGAATTATGTTGATAAATTCCGAGAAAAGATCCTGTCATCACCCTACAGCTCC
  TATCTTCAGCAAGAAAGCAGGAGCAAAGCTAAATATTCATATTTTCAAGGAGATTCTACTTCCAAGCAG
  ACGCGGTCGGCCGGCTGCAGGAAAGGGAAGCACAAGCGGAAGAAGCTGCCGGAGCCGCCAGACAGCAGC
  AGCTCGAACACCGGCTCTGGTCCCCGCAGGGGAGCGCATCAGAACGCACAGCCCTGCTGCCCCTCCGCG
  GCCTCCTCTCCGCACACCTCGAGCCCGACCTTCCCACCTGCCGCCATGGTGCCCAGCCAGGCCCCTTAC
  CTCGTCCCAGCTTTTCCCCTCCCAGCCGCGACCTCACCCGGAAGAGAATACGCAGCCCCCGGAACTGCA
  CCGGAAGGCCTGCATGGGCTGCCCTTGTCCGAGGGCTTGCAGCCTTACCCAGCTTTCCCTTTTCCTTAC
  TTGGATACTTTTATGACCGTTTTCCTGCCTGACCCCCCTGTCTGTCCTCTGTTGTCGCCATCGTTTTTG
  CCATGTCCATTCCTGGGGGCGACAGCCTCTTCTGCGATATCACCCTCAATGTCGTCAGCAATGAGTCCA
  ACTCTGGACCCACCCCCTTCAGTCACCAGCCAAAGGAGAGAGGAGGAAAAGTGGGAGGCACAAAGCGAG
  GGGCACCCGTTCATTACTTCGAGAAGCAGCTCACCCTTGCAGTTAAACTTACTTCAGGAAGAGATGCCC
  AGACCCTCTGAATCTCCAGATCAGATGAGAAGGAACACGTGCCCACAAACTGAGTATTGTGTTACAGGC
  AACAATGGCAGTGAGAGCAGTCCTGCTACTACCGGTGCACTGTCCACGGGGTCACCTCCCAGGGAGAAT
  CCATCCCATCCTACTGCCAGCGCTCTGTCCACAGGATCGCCTCCCATGAAGAATCCATCCCATCCTACT
  GCCAGCACACTGTCCATGGGATTGCCTCCCAGCAGGACTCCATCCCATCCTACTGCCACTGTTCTGTCC
  ACGGGGTCACCTCCCAGCGAATCCCCATCCAGAACTGGTTCAGCAGCATCAGGAAGCAGCGACAGCAGT
  ATATACCTTACTAGTAGTGTTTATTCTTCTAAAATCTCCCAAAATGGGCAGCAATCTCAGGACGTACAG
  AAAAAAGAAACATTTCCTAATGTCGCCGAAGAGCCCATCTGGAGAATGATACGGCAGACACCTGAGCGC
  ATTCTCATGACATACCAGGTACCTGAGAGGGTTAAAGAAGTTGTACTAAAAGAAGACCTGGAAAAGCTA
  GAAAGTATGAGGCAGCAGCAGCCCCAGTTTTCTCATGGGCAAAAGGAGGAGCTGGCTAAGGTGTATAAT
  TGGATTCAAAGCCAGACTGTCACTCAAGAAATCGACATTCAAGCCTGTGTCACTTGTGAAAATGAAGAT
  TCAGCTGATGGTGCGGCCACATCCTGTGGTCAGGTTCTGGTAGAAGACAGCTGT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240035
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPRGEAPGPGRRGAKDEALGEESGERWSPEFHLQRKLADSSHSEQQDRNRVSEELIMVVQEMKKYFPSE
  RRNKPSTLDALNYALRCVHSVQANSEFFQILSQNGAPQADVSMYSLEELATIASEHTSKNTDTFVAVFS
  FLSGRLVHISEQAALILNRKKDVLASSHFVDLLAPQDMRVFYAHTARAQLPFWNNWTQRAAARYECAPV
  KPFFCRIRGGEDRKQEKCHSPFRIIPYLIHVHHPAQPELESEPCCLTVVEKIHSGYEAPRIPVNKRIFT
  TTHTPGCVFLEVDEKAVPLLGYLPQDLIGTSILSYLHPEDRSLMVAIHQKVLKYAGHPPFEHSPIRFCT
  QNGDYIILDSSWSSFVNPWSRKISFIIGRHKVRTSPLNEDVFATKIKKMNDNDKDITELQEQIYKLLLQ
  PVHVSVSSGYGSLGSSGSQEQLVSIASSSEASGHRVEETKAEQMTLQQVYASVNKIKNLGQQLYIESMT
  KSSFKPVTGTRTEPNGGGESANGGGECKTFTSFHQTLKNNSVYTEPCEDLRNDEHSPSYQQINCIDSVI
  RYLKSYNIPALKRKCISCTNTTSSSSEEDKQNHKADDVQALQAGLQIPAIPKSEMPTNGRSIDTGGGAP
  QILSTAMLSLGSGISQCGYSSTIVHVPPPETARDATLFCEPWTLNMQPAPLTSEEFKHVGLTAAVLSAH
  TQKEEQNYVDKFREKILSSPYSSYLQQESRSKAKYSYFQGDSTSKQTRSAGCRKGKHKRKKLPEPPDSS
  SSNTGSGPRRGAHQNAQPCCPSAASSPHTSSPTFPPAAMVPSQAPYLVPAFPLPAATSPGREYAAPGTA
  PEGLHGLPLSEGLQPYPAFPFPYLDTFMTVFLPDPPVCPLLSPSFLPCPFLGATASSAISPSMSSAMSP
  TLDPPPSVTSQRREEEKWEAQSEGHPFITSRSSSPLQLNLLQEEMPRPSESPDQMRRNTCPQTEYCVTG
  NNGSESSPATTGALSTGSPPRENPSHPTASALSTGSPPMKNPSHPTASTLSMGLPPSRTPSHPTATVLS
  TGSPPSESPSRTGSAASGSSDSSIYLTSSVYSSKISQNGQQSQDVQKKETFPNVAEEPIWRMIRQTPER
  ILMTYQVPERVKEVVLKEDLEKLESMRQQQPQFSHGQKEELAKVYNWIQSQTVTQEIDIQACVTCENED
  SADGAATSCGQVLVEDSC
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289861
• ORF Size: 3573 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001289861.2
• RefSeq Size: 6279 bp
• RefSeq ORF: 3576 bp
• Locus ID: 8863
• UniProt ID: P56645
• Cytogenetics: 1p36.23
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• MW: 131.2 kDa
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]