alpha 1 Catenin (CTNNA1) (NM_001290307) Human Tagged ORF Clone

CAT#: RG239664

CTNNA1 (tGFP-tagged) - Human catenin (cadherin-associated protein), alpha 1, 102kDa (CTNNA1), transcript variant 2

Product Images

Circular map for RG239664

Specifications

Product Data

• Tag: TurboGFP
• Symbol: alpha 1 Catenin
• Synonyms: CAP102; MDPT2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239664 representing NM_001290307.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACTGCTGTCCATGCAGGCAACATAAACTTCAAGTGGGATCCTAAAAGTCTAGAGATCAGGACTCTG
  GCAGTTGAGAGACTGTTGGAGCCTCTTGTTACACAGGTTACAACCCTTGTAAACACCAATAGTAAAGGG
  CCCTCTAATAAGAAGAGAGGTCGTTCTAAGAAGGCCCATGTTTTGGCTGCATCTGTTGAACAAGCAACT
  GAGAATTTCTTGGAGAAGGGGGATAAAATTGCGAAGGAGAGCCAGTTTCTCAAGGAGGAGCTTGTGGCT
  GCTGTAGAAGATGTTCGAAAACAAGGTGATTTGATGAAGGCTGCTGCAGGAGAGTTCGCAGATGATCCC
  TGCTCTTCTGTGAAGCGAGGCAACATGGTTCGGGCAGCTCGAGCTTTGCTCTCTGCTGTTACCCGGTTG
  CTGATTTTGGCTGACATGGCAGATGTCTACAAATTACTTGTTCAGCTGAAAGTTGTGGAAGATGGTATC
  TTGAAGTTGAGGAATGCTGGCAATGAACAAGACTTAGGAATCCAGTATAAAGCCCTAAAACCTGAAGTG
  GATAAGCTGAACATTATGGCAGCCAAAAGACAACAGGAATTGAAAGATGTTGGCCATCGTGATCAGATG
  GCTGCAGCTAGAGGAATCCTGCAGAAGAACGTTCCGATCCTCTATACTGCATCCCAGGCATGCCTACAG
  CACCCTGATGTCGCAGCCTATAAGGCCAACAGGGACCTGATATACAAGCAGCTGCAGCAGGCGGTCACA
  GGCATTTCCAATGCAGCCCAGGCCACTGCCTCAGACGATGCCTCACAGCACCAGGGTGGAGGAGGAGGA
  GAACTGGCATATGCACTCAATAACTTTGACAAACAAATCATTGTGGACCCCTTGAGCTTCAGCGAGGAG
  CGCTTTAGGCCTTCCCTGGAGGAGCGTCTGGAAAGCATCATTAGTGGGGCTGCCTTGATGGCCGACTCG
  TCCTGCACGCGTGATGACCGTCGTGAGCGAATTGTGGCAGAGTGTAATGCTGTCCGCCAGGCCCTGCAG
  GACCTGCTTTCGGAGTACATGGGCAATGCTGGACGTAAAGAAAGAAGTGATGCACTCAATTCTGCAATA
  GATAAAATGACCAAGAAGACCAGGGACTTGCGTAGACAGCTCCGCAAAGCTGTCATGGACCACGTTTCA
  GATTCTTTCCTGGAAACCAATGTTCCACTTTTGGTATTGATTGAAGCTGCAAAGAATGGAAATGAGAAA
  GAAGTTAAGGAGTATGCCCAAGTTTTCCGTGAACATGCCAACAAATTGATTGAGGTTGCCAACTTGGCC
  TGTTCCATCTCAAATAATGAAGAAGGTGTAAAGCTTGTTCGAATGTCTGCAAGCCAGTTAGAAGCCCTC
  TGTCCTCAGGTTATTAATGCTGCACTGGCTTTAGCAGCAAAACCACAGAGTAAACTGGCCCAAGAGAAC
  ATGGATCTTTTTAAAGAACAATGGGAAAAACAAGTCCGTGTTCTCACAGATGCTGTCGATGACATTACT
  TCCATTGATGACTTCTTGGCTGTCTCAGAGAATCACATTTTGGAAGATGTGAACAAATGTGTCATTGCT
  CTCCAAGAGAAGGATGTGGATGGCCTGGACCGCACAGCTGGTGCAATTCGAGGCCGGGCAGCCCGGGTC
  ATTCACGTAGTCACCTCAGAGATGGACAACTATGAGCCAGGAGTCTACACAGAGAAGGTTCTGGAAGCC
  ACTAAGCTGCTCTCCAACACAGTCATGCCACGTTTTACTGAGCAAGTAGAAGCAGCCGTGGAAGCCCTC
  AGCTCGGACCCTGCCCAGCCCATGGATGAGAATGAGTTTATCGATGCTTCCCGCCTGGTATATGATGGC
  ATCCGGGACATCAGGAAAGCAGTGCTGATGATAAGGACCCCTGAGGAGTTGGATGACTCTGACTTTGAG
  ACAGAAGATTTTGATGTCAGAAGCAGGACGAGCGTCCAGACAGAAGACGATCAGCTGATAGCTGGCCAG
  AGTGCCCGGGCGATCATGGCTCAGCTTCCCCAGGAGCAAAAAGCGAAGATTGCGGAACAGGTGGCCAGC
  TTCCAGGAAGAAAAGAGCAAGCTGGATGCTGAAGTGTCCAAATGGGACGACAGTGGCAATGACATCATT
  GTGCTGGCCAAGCAGATGTGCATGATTATGATGGAGATGACAGACTTTACCCGAGGTAAAGGACCACTC
  AAAAATACATCGGATGTCATCAGTGCTGCCAAGAAAATTGCTGAGGCAGGATCCAGGATGGACAAGCTT
  GGCCGCACCATTGCAGACCATTGCCCCGACTCGGCTTGCAAGCAGGACCTGCTGGCCTACCTGCAACGC
  ATCGCCCTCTACTGCCACCAGCTGAACATCTGCAGCAAGGTCAAGGCCGAGGTGCAGAATCTCGGCGGG
  GAGCTTGTTGTCTCTGGGAACTGTGACACCTGCGGGGCACTGCAAGGGCTGAAAGGCTGGCCTCCTCCC
  CTTTGCTGGCCACTCACTGGGTGGACAGCGCCATGTCCC
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239664
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MTAVHAGNINFKWDPKSLEIRTLAVERLLEPLVTQVTTLVNTNSKGPSNKKRGRSKKAHVLAASVEQAT
  ENFLEKGDKIAKESQFLKEELVAAVEDVRKQGDLMKAAAGEFADDPCSSVKRGNMVRAARALLSAVTRL
  LILADMADVYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKALKPEVDKLNIMAAKRQQELKDVGHRDQM
  AAARGILQKNVPILYTASQACLQHPDVAAYKANRDLIYKQLQQAVTGISNAAQATASDDASQHQGGGGG
  ELAYALNNFDKQIIVDPLSFSEERFRPSLEERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQ
  DLLSEYMGNAGRKERSDALNSAIDKMTKKTRDLRRQLRKAVMDHVSDSFLETNVPLLVLIEAAKNGNEK
  EVKEYAQVFREHANKLIEVANLACSISNNEEGVKLVRMSASQLEALCPQVINAALALAAKPQSKLAQEN
  MDLFKEQWEKQVRVLTDAVDDITSIDDFLAVSENHILEDVNKCVIALQEKDVDGLDRTAGAIRGRAARV
  IHVVTSEMDNYEPGVYTEKVLEATKLLSNTVMPRFTEQVEAAVEALSSDPAQPMDENEFIDASRLVYDG
  IRDIRKAVLMIRTPEELDDSDFETEDFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQVAS
  FQEEKSKLDAEVSKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKL
  GRTIADHCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELVVSGNCDTCGALQGLKGWPPP
  LCWPLTGWTAPCP
  SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL
  SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF
  PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP
  MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290307
• ORF Size: 2523 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001290307.2, NP_001277236.1
• RefSeq Size: 3862 bp
• RefSeq ORF: 2526 bp
• Locus ID: 1495
• UniProt ID: P35221
• Cytogenetics: 5q31.2
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Endometrial cancer, Leukocyte transendothelial migration, Pathways in cancer, Tight junction
• MW: 93.2 kDa
• Gene Summary: This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016]