TAP2 (NM_001290043) Human Tagged ORF Clone

CAT#: RG239305

TAP2 (tGFP-tagged) - Human transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2), transcript variant 1, A allele

Product Images

Circular map for RG239305

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TAP2
• Synonyms: ABC18; ABCB3; APT2; D6S217E; PSF-2; PSF2; RING11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239305 representing NM_001290043.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCGGCTCCCTGACCTGAGACCCTGGACCTCCCTGCTGCTGGTGGACGCGGCTTTACTGTGGCTGCTT
  CAGGGCCCTCTGGGGACTTTGCTTCCTCAAGGGCTGCCAGGACTATGGCTGGAGGGGACCCTGCGGCTG
  GGAGGGCTGTGGGGGCTGCTAAAGCTAAGAGGGCTGCTGGGATTTGTGGGGACACTGCTGCTCCCGCTC
  TGTCTGGCCACCCCCCTGACTGTCTCCCTGAGAGCCCTGGTCGCGGGGGCCTCACGTGCTCCCCCAGCC
  AGAGTCGCTTCAGCCCCTTGGAGCTGGCTGCTGGTGGGGTACGGGGCTGCGGGGCTCAGCTGGTCACTG
  TGGGCTGTTCTGAGCCCTCCTGGAGCCCAGGAGAAGGAGCAGGACCAGGTGAACAACAAAGTCTTGATG
  TGGAGGCTGCTGAAGCTCTCCAGGCCGGACCTGCCTCTCCTCGTTGCCGCCTTCTTCTTCCTTGTCCTT
  GCTGTTTTGGGTGAGACATTAATCCCTCACTATTCTGGTCGTGTGATTGACATCCTGGGAGGTGATTTT
  GACCCCCATGCCTTTGCCAGTGCCATCTTCTTCATGTGCCTCTTCTCCTTTGGCAGCTCACTGTCTGCA
  GGCTGCCGAGGAGGCTGCTTCACCTACACCATGTCTCGAATCAACTTGCGGATCCGGGAGCAGCTTTTC
  TCCTCCCTGCTGCGCCAGGACCTCGGTTTCTTCCAGGAGACTAAGACAGGGGAGCTGAACTCACGGCTG
  AGCTCGGATACCACCCTGATGAGTAACTGGCTTCCTTTAAATGCCAATGTGCTCTTGCGAAGCCTGGTG
  AAAGTGGTGGGGCTGTATGGCTTCATGCTCAGCATATCGCCTCGACTCACCCTCCTTTCTCTGCTGCAC
  ATGCCCTTCACAATAGCAGCGGAGAAGGTGTACAACACCCGCCATCAGGAAGTGCTTCGGGAGATCCAG
  GATGCAGTGGCCAGGGCGGGGCAGGTGGTGCGGGAAGCCGTTGGAGGGCTGCAGACCGTTCGCAGTTTT
  GGGGCCGAGGAGCATGAAGTCTGTCGCTATAAAGAGGCCCTTGAACAATGTCGGCAGCTGTATTGGCGG
  AGAGACCTGGAACGCGCCTTGTACCTGCTCGTAAGGAGGGTGCTGCACTTGGGGGTGCAGATGCTGATG
  CTGAGCTGTGGGCTGCAGCAGATGCAGGATGGGGAGCTCACCCAGGGCAGCCTGCTTTCCTTTATGATC
  TACCAGGAGAGCGTGGGGAGCTATGTGCAGACCCTGGTATACATATATGGGGATATGCTCAGCAACGTG
  GGAGCTGCAGAGAAGGTTTTCTCCTACATGGACCGACAGCCAAATCTGCCTTCACCTGGCACGCTTGCC
  CCCACCACTCTGCAGGGGGTTGTGAAATTCCAAGACGTCTCCTTTGCATATCCCAATCGCCCTGACAGG
  CCTGTGCTCAAGGGGCTGACGTTTACCCTACGTCCTGGTGAGGTGACGGCGCTGGTGGGACCCAATGGG
  TCTGGGAAGAGCACAGTGGCTGCCCTGCTGCAGAATCTGTACCAGCCCACAGGGGGACAGGTGCTGCTG
  GATGAAAAGCCCATCTCACAGTATGAACACTGCTACCTGCACAGCCAGGTGGTTTCAGTTGGGCAGGAG
  CCTGTGCTGTTCTCCGGTTCTGTGAGGAACAACATTGCTTATGGGCTGCAGAGCTGCGAAGATGATAAG
  GTGATGGCGGCTGCCCAGGCTGCCCACGCAGATGACTTCATCCAGGAAATGGAGCATGGAATATACACA
  GATGTAGGGGAGAAGGGAAGCCAGCTGGCTGCGGGACAGAAACAACGTCTGGCCATTGCCCGGGCCCTT
  GTACGAGACCCGCGGGTCCTCATCCTGGATGAGGCTACTAGTGCCCTAGATGTGCAGTGCGAGCAGGCC
  CTGCAGGACTGGAATTCCCGTGGGGATCGCACAGTGCTGGTGATTGCTCACAGGCTGCAGACAGTTCAG
  CGCGCCCACCAGATCCTGGTGCTCCAGGAGGGCAAGCTGCAGAAGCTTGCCCAGCTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239305
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRLPDLRPWTSLLLVDAALLWLLQGPLGTLLPQGLPGLWLEGTLRLGGLWGLLKLRGLLGFVGTLLLPL
  CLATPLTVSLRALVAGASRAPPARVASAPWSWLLVGYGAAGLSWSLWAVLSPPGAQEKEQDQVNNKVLM
  WRLLKLSRPDLPLLVAAFFFLVLAVLGETLIPHYSGRVIDILGGDFDPHAFASAIFFMCLFSFGSSLSA
  GCRGGCFTYTMSRINLRIREQLFSSLLRQDLGFFQETKTGELNSRLSSDTTLMSNWLPLNANVLLRSLV
  KVVGLYGFMLSISPRLTLLSLLHMPFTIAAEKVYNTRHQEVLREIQDAVARAGQVVREAVGGLQTVRSF
  GAEEHEVCRYKEALEQCRQLYWRRDLERALYLLVRRVLHLGVQMLMLSCGLQQMQDGELTQGSLLSFMI
  YQESVGSYVQTLVYIYGDMLSNVGAAEKVFSYMDRQPNLPSPGTLAPTTLQGVVKFQDVSFAYPNRPDR
  PVLKGLTFTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGGQVLLDEKPISQYEHCYLHSQVVSVGQE
  PVLFSGSVRNNIAYGLQSCEDDKVMAAAQAAHADDFIQEMEHGIYTDVGEKGSQLAAGQKQRLAIARAL
  VRDPRVLILDEATSALDVQCEQALQDWNSRGDRTVLVIAHRLQTVQRAHQILVLQEGKLQKLAQL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290043
• ORF Size: 2058 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001290043.2
• RefSeq Size: 5732 bp
• RefSeq ORF: 2061 bp
• Locus ID: 6891
• UniProt ID: Q03519
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters, Antigen processing and presentation, Primary immunodeficiency
• MW: 75.7 kDa
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules. [provided by RefSeq, Feb 2014]