GGT5 (NM_001302464) Human Tagged ORF Clone

CAT#: RG238758

GGT5 (tGFP-tagged) - Human gamma-glutamyltransferase 5 (GGT5), transcript variant 4

Product Images

Circular map for RG238758

Specifications

Product Data

• Tag: TurboGFP
• Symbol: GGT5
• Synonyms: GGL; GGT-REL; GGT 5; GGTLA1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238758 representing NM_001302464.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCCGGGGCTACGGGGCCACGGTCAGCCTAGTCCTGCTGGGTCTGGGGCTGGCGCTGGCTGTCATT
  GTGCTGGCTGTGGTCCTCTCTCGACACCAGGCCCCATGTGGCCCCCAGGCCTTTGCCCACGCTGCTGTT
  GCCGCCGACTCCAAGGTCTGCTCGGATATTGGACGAGCCATCCTCCAGCAGCAGGGCTCACCCGTGGAT
  GCCACCATCGCGGCTCTGGTCTGCACCAGCGTCGTCAACCCTCAGAGCATGGGCCTGGGCGGAGGGGTC
  ATCTTCACCATCTACAATGTGACAACAGGGGCCCAGTGGATCGGGGTGCCCGGGGAGCTCCGTGGCTAT
  GCCGAGGCCCACCGCCGCCATGGCCGCCTGCCCTGGGCGCAGCTGTTCCAGCCCACCATCGCGCTGCTC
  CGAGGGGGGCATGTGGTGGCCCCTGTCCTCAGCCGTTTCCTGCACAACAGCATCCTGCGGCCTTCCTTG
  CAGGCGTCAACCCTGCGCCAGCTCTTCTTCAACGGGACAGAACCCCTGAGGCCTCAGGACCCACTCCCA
  TGGCCTGCACTGGCCACCACCCTGGAGACCGTGGCCACAGAGGGCGTGGAGGTCTTCTACACGGGGAGG
  CTGGGCCAGATGCTGGTGGAGGACATTGCCAAGGAAGGGAGCCAGCTGACGCTGCAGGACCTGGCCAAG
  TTCCAGCCCGAGGTGGTGGATGCCCTGGAGGTGCCCCTGGGGGACTATACCCTGTACTCACCACCGCCG
  CCTGCAGGGGGTGCCATTCTCAGCTTTATCCTCAACGTGCTAAGAGGGTTCAACTTCTCAACAGAGTCT
  ATGGCCAGGCCTGAAGGGAGGGTGAACGTGTACCACCACCTTGTAGAGACGCTCAAGTTTGCCAAGGGG
  CAGAGGTGGAGGCTGGGGGACCCTCGAAGCCACCCGAAGCTCCAGAATGCCTCCCGGGACCTGCTGGGG
  GAGACCCTGGCCCAGCTCATCCGCCAACAGATCGATGGCCGGGGGGACCACCAGCTCAGCCACTACAGC
  TTGGCCGAGGCCTGGGGCCACGGGACAGGCACGTCCCATGTGTCTGTGCTGGGGGAGGATGGCAGCGCC
  GTGGCTGCCACCAGCACCATCAACACACCCTTTGGAGCGATGGTGTATTCACCACGGACAGGCATCATC
  CTCAACAACGAGCTCCTGGACTTATGCGAGCGATGCCCCCGGGGTTCCGGCACCACCCCCTCACCTGTG
  AGTGGAGACAGGGTGGGTGGAGCTCCCGGAAGGTGCTGGCCCCCAGTTCCAGGCGAGCGTTCCCCATCC
  TCCATGGTGCCCTCCATCTTGATCAACAAAGCCCAGGGGTCGAAGCTAGTGATTGGCGGGGCTGGCGGG
  GAGCTCATCATCTCTGCTGTGGCCCAGGCCATCATGAGCAAGCTGTGGCTTGGCTTTGACCTGAGAGCG
  GCCATTGCAGCCCCCATCCTGCATGTCAACAGCAAGGGCTGTGTGGAGTACGAGCCCAACTTCAGCCAG
  AGACGGTGTCTTGCTTTGTTGCCCTGGCTGTTCTCAAACTCCTGGCCTCAAGTGATCCTGCCACCTTGG
  CTCCCAAGTGGC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238758
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MARGYGATVSLVLLGLGLALAVIVLAVVLSRHQAPCGPQAFAHAAVAADSKVCSDIGRAILQQQGSPVD
  ATIAALVCTSVVNPQSMGLGGGVIFTIYNVTTGAQWIGVPGELRGYAEAHRRHGRLPWAQLFQPTIALL
  RGGHVVAPVLSRFLHNSILRPSLQASTLRQLFFNGTEPLRPQDPLPWPALATTLETVATEGVEVFYTGR
  LGQMLVEDIAKEGSQLTLQDLAKFQPEVVDALEVPLGDYTLYSPPPPAGGAILSFILNVLRGFNFSTES
  MARPEGRVNVYHHLVETLKFAKGQRWRLGDPRSHPKLQNASRDLLGETLAQLIRQQIDGRGDHQLSHYS
  LAEAWGHGTGTSHVSVLGEDGSAVAATSTINTPFGAMVYSPRTGIILNNELLDLCERCPRGSGTTPSPV
  SGDRVGGAPGRCWPPVPGERSPSSMVPSILINKAQGSKLVIGGAGGELIISAVAQAIMSKLWLGFDLRA
  AIAAPILHVNSKGCVEYEPNFSQRRCLALLPWLFSNSWPQVILPPWLPSG
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302464
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001302464.1, NP_001289393.1
• RefSeq Size: 2507 bp
• RefSeq ORF: 1602 bp
• Locus ID: 2687
• UniProt ID: P36269
• Cytogenetics: 22q11.23
• Protein Families: Protease, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Cyanoamino acid metabolism, Glutathione metabolism, Metabolic pathways, Selenoamino acid metabolism, Taurine and hypotaurine metabolism
• MW: 57.3 kDa
• Gene Summary: This gene is a member of the gamma-glutamyl transpeptidase gene family, and some reports indicate that it is capable of cleaving the gamma-glutamyl moiety of glutathione. The protein encoded by this gene is synthesized as a single, catalytically-inactive polypeptide, that is processed post-transcriptionally to form a heavy and light subunit, with the catalytic activity contained within the small subunit. The encoded enzyme is able to convert leukotriene C4 to leukotriene D4, but appears to have distinct substrate specificity compared to gamma-glutamyl transpeptidase. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]