TMEM147 (NM_001242598) Human Tagged ORF Clone

CAT#: RG236077

TMEM147 (tGFP-tagged) - Human transmembrane protein 147 (TMEM147), transcript variant 3

Product Images

Circular map for RG236077

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TMEM147
• Synonyms: NIFIE14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG236077 representing NM_001242598.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACCCTGTTTCACTTCGGGAACTGCTTCGCTCTTGCCTACTTCCCCTACTTCATCACCTACAAGTGC
  AGCGGCCTGTCCGAGTACAACGCCTTCTGGAAATGCGTCCAGGCTGGAGTCACCTACCTCTTTGTCCAA
  CTCTGCAAGATGCTGTTCTTGGCCACTTTCTTTCCCACCTGGGAAGGCGGCATCTATGACTTCATTGGG
  GTCCATTACATCGTCGCGTCTGCTCAGGTCTGGATGATAACACGCTATGATCTGTACCACACCTTCCGG
  CCAGCTGTCCTCCTGCTGATGTTCCTCAGTGTCTACAAGGCCTTTGTTATGGAGACCTTCGTCCACCTC
  TGCTCGCTGGGCAGTTGGGCAGCTCTACTGGCCCGAGCAGTGGTAACGGGGCTGCTGGCCCTCAGCACT
  TTGGCCCTGTATGTCGCCGTTGTCAATGTGCACTCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG236077
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MTLFHFGNCFALAYFPYFITYKCSGLSEYNAFWKCVQAGVTYLFVQLCKMLFLATFFPTWEGGIYDFIG
  VHYIVASAQVWMITRYDLYHTFRPAVLLLMFLSVYKAFVMETFVHLCSLGSWAALLARAVVTGLLALST
  LALYVAVVNVHS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242598
• ORF Size: 450 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001242598.2
• RefSeq Size: 687 bp
• RefSeq ORF: 453 bp
• Locus ID: 10430
• Cytogenetics: 19q13.12
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 17.5 kDa
• Gene Summary: Acts as a negative regulator of CHRM3 function, most likely by interfering with its trafficking to the cell membrane (PubMed:21056967). Negatively regulates CHRM3-mediated calcium mobilization and activation of RPS6KA1/p90RSK activity (PubMed:21056967).[UniProtKB/Swiss-Prot Function]