BORIS (CTCFL) (NM_001269048) Human Tagged ORF Clone

CAT#: RG234234

CTCFL (tGFP-tagged) - Homo sapiens CCCTC-binding factor (zinc finger protein)-like (CTCFL), transcript variant 10

Product Images

Circular map for RG234234

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BORIS
• Synonyms: BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234234 representing NM_001269048
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCACTGAGATCTCTGTCCTTTCTGAGCAATTCACCAAGATCAAAGAACTCGAGTTGATGCCGG
  AAAAAGGCCTGAAGGAGGAGGAAAAAGACGGAGTGTGCAGAGAGAAAGACCATCGGAGCCCTAGTGAGTT
  GGAGGCCGAGCGTACCTCTGGGGCCTTCCAGGACAGCGTCCTGGAGGAAGAAGTGGAGCTGGTGCTGGCC
  CCCTCGGAGGAGAGCGAGAAGTACATCCTGACCCTGCAGACGGTGCACTTCACTTCTGAAGCTGTGGAGT
  TGCAGGATATGAGCTTGCTGAGCATACAGCAGCAAGAAGGGGTGCAGGTGGTGGTGCAACAGCCTGGCCC
  TGGGTTGCTGTGGCTTGAGGAAGGGCCCCGGCAGAGCCTGCAGCAGTGTGTGGCCATTAGTATCCAGCAA
  GAGCTGTACTCCCCGCAAGAGATGGAGGTGTTGCAGTTCCACGCTCTAGAGGAGAATGTGATGGTGGCCA
  GTGAAGACAGTAAGTTAGCGGTGAGCCTGGCTGAAACTACTGGACTGATCAAGCTCGAGGAAGAGCAGGA
  GAAGAACCAGTTATTGGCTGAAAGAACAAAGGAGCAGCTCTTTTTTGTGGAAACAATGTCAGGAGATGAA
  AGAAGTGACGAAATTGTTCTCACAGTTTCAAATTCAAATGTGGAAGAACAAGAGGATCAACCTACAGCTG
  GTCAAGCAGATGCTGAAAAGGCCAAATCTACAAAAAATCAAAGAAAGACAAAGGGAGCAAAAGGAACCTT
  CCACTGTGATGTCTGCATGTTCACCTCTTCTAGAATGTCAAGTTTTAATCGTCATATGAAAACTCACACC
  AGTGAGAAGCCTCACCTGTGTCACCTCTGCCTGAAAACCTTCCGTACGGTCACTCTGCTGCGGAACCATG
  TTAACACCCACACAGGAACCAGGCCCTACAAGTGTAACGACTGCAACATGGCATTTGTCACCAGTGGAGA
  ACTCGTCCGACACAGGCGCTATAAACATACTCATGAGAAACCCTTTAAATGTTCCATGTGCAAGTATGCC
  AGTGTGGAGGAACGTCATATGACCGCTCACATTCGTACCCACACTGGAGAGAAACCATTCACCTGCCTTT
  CTTGCAATAAATGTTTCCGACAGAAGCAACTTCTAAACGCTCACTTCAGGAAATACCACGATGCAAATTT
  CATCCCGACTGTTTACAAATGCTCCAAGTGTGGCAAAGGCTTTTCCCGCTGGATTCTCTGGGTTGGGAAC
  TCGGAAGTGGCTGAACTGGGTGGTCCTGGCTCAGGGCCACTCCTGAGGCTGCAGTCAGGATGTCCGCCAG
  GGCTGCATCATCCGAAGGCTGGACTGGGGCCAGAGGATCCACTTCCAGGACAGCTCCGCCACACAACTGC
  TGGCACCGGCCTCAGTTCCTTGCTACAGGGACCTCTCTGCAGGGCTGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234234 representing NM_001269048
  Red=Cloning site Green=Tags(s)
  
  MAATEISVLSEQFTKIKELELMPEKGLKEEEKDGVCREKDHRSPSELEAERTSGAFQDSVLEEEVELVLA
  PSEESEKYILTLQTVHFTSEAVELQDMSLLSIQQQEGVQVVVQQPGPGLLWLEEGPRQSLQQCVAISIQQ
  ELYSPQEMEVLQFHALEENVMVASEDSKLAVSLAETTGLIKLEEEQEKNQLLAERTKEQLFFVETMSGDE
  RSDEIVLTVSNSNVEEQEDQPTAGQADAEKAKSTKNQRKTKGAKGTFHCDVCMFTSSRMSSFNRHMKTHT
  SEKPHLCHLCLKTFRTVTLLRNHVNTHTGTRPYKCNDCNMAFVTSGELVRHRRYKHTHEKPFKCSMCKYA
  SVEERHMTAHIRTHTGEKPFTCLSCNKCFRQKQLLNAHFRKYHDANFIPTVYKCSKCGKGFSRWILWVGN
  SEVAELGGPGSGPLLRLQSGCPPGLHHPKAGLGPEDPLPGQLRHTTAGTGLSSLLQGPLCRAA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001269048
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001269048.1, NP_001255977.1
• RefSeq Size: 2995 bp
• RefSeq ORF: 1452 bp
• Locus ID: 140690
• UniProt ID: Q8NI51
• Cytogenetics: 20q13.31
• Protein Families: Transcription Factors
• Gene Summary: CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]