BORIS (CTCFL) (NM_001269054) Human Tagged ORF Clone

CAT#: RG233995

CTCFL (tGFP-tagged) - Homo sapiens CCCTC-binding factor (zinc finger protein)-like (CTCFL), transcript variant 15

Product Images

Circular map for RG233995

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BORIS
• Synonyms: BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233995 representing NM_001269054
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACCTCTTCTAGAATGTCAAGTTTTAATCGTCATATGAAAACTCACACCAGTGAGAAGCCTCACC
  TGTGTCACCTCTGCCTGAAAACCTTCCGTACGGTCACTCTGCTGCGGAACCATGTTAACACCCACACAGG
  AACCAGGCCCTACAAGTGTAACGACTGCAACATGGCATTTGTCACCAGTGGAGAACTCGTCCGACACAGG
  CGCTATAAACATACTCATGAGAAACCCTTTAAATGTTCCATGTGCAAGTATGCCAGTGTGGAGGCAAGTA
  AATTGAAGCGCCATGTCCGATCCCACACTGGGGAGCGCCCCTTTCAGTGTTGCCAGTGCAGCTATGCCAG
  CAGAGATACCTACAAGCTGAAACGCCACATGAGAACGCACTCAGGTGAGAAGCCTTACGAATGCCACATC
  TGCCACACCCGCTTCACCCAGAGCGGGACCATGAAAATACATATTCTGCAGAAACACGGCGAAAATGTCC
  CCAAATACCAGTGTCCCCATTGTGCCACCATCATTGCACGGAAAAGCGACCTACGTGTGCATATGCGCAA
  CTTGCATGCTTACAGCGCTGCAGAGCTGAAATGCCGCTACTGTTCTGCTGTCTTCCATGAACGCTATGCC
  CTCATTCAGCACCAGAAAACTCATAAGAATGAGAAGAGGTTCAAGTGCAAACACTGCAGTTATGCCTGCA
  AGCAGGAACGTCATATGACCGCTCACATTCGTACCCACACTGGAGAGAAACCATTCACCTGCCTTTCTTG
  CAATAAATGTTTCCGACAGAAGCAACTTCTAAACGCTCACTTCAGGAAATACCACGATGCAAATTTCATC
  CCGACTGTTTACAAATGCTCCAAGTGTGGCAAAGGCTTTTCCCGCTGGATTAACCTGCACAGACATTCGG
  AGAAGTGTGGATCAGGGGAAGCAAAGTCGGCTGCTTCAGGAAAGGGAAGAAGAACAAGAAAGAGGAAGCA
  GACCATCCTGAAGGAAGCCACAAAGGGTCAGAAGGAAGCTGCGAAGGGATGGAAGGAAGCCGCGAACGGA
  GACGGTGTGATCTCAGCTCACCGCAACCTCTGCCTCCTGGGTTCAAGTGATTCTCATGCCTCAGTCTCCG
  GAGCTGGGATTACAGATGCCCGCCACCACGCCTGGCTAATTGTTCTATTATTTTTAGTAGAGATGGGGTT
  TTACCATGTCTCTCACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233995 representing NM_001269054
  Red=Cloning site Green=Tags(s)
  
  MFTSSRMSSFNRHMKTHTSEKPHLCHLCLKTFRTVTLLRNHVNTHTGTRPYKCNDCNMAFVTSGELVRHR
  RYKHTHEKPFKCSMCKYASVEASKLKRHVRSHTGERPFQCCQCSYASRDTYKLKRHMRTHSGEKPYECHI
  CHTRFTQSGTMKIHILQKHGENVPKYQCPHCATIIARKSDLRVHMRNLHAYSAAELKCRYCSAVFHERYA
  LIQHQKTHKNEKRFKCKHCSYACKQERHMTAHIRTHTGEKPFTCLSCNKCFRQKQLLNAHFRKYHDANFI
  PTVYKCSKCGKGFSRWINLHRHSEKCGSGEAKSAASGKGRRTRKRKQTILKEATKGQKEAAKGWKEAANG
  DGVISAHRNLCLLGSSDSHASVSGAGITDARHHAWLIVLLFLVEMGFYHVSHS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001269054
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001269054.1, NP_001255983.1
• RefSeq Size: 2077 bp
• RefSeq ORF: 1212 bp
• Locus ID: 140690
• UniProt ID: Q8NI51
• Cytogenetics: 20q13.31
• Protein Families: Transcription Factors
• Gene Summary: CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]