UPB1 (NM_016327) Human Tagged ORF Clone

CAT#: RG223365

UPB1 (tGFP-tagged) - Human ureidopropionase, beta (UPB1)

Product Images

Circular map for RG223365

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UPB1
• Synonyms: BUP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223365 representing NM_016327
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGCTGAGTGGAAGTCGCTGGAGGAATGCTTGGAGAAGCACCTGCCGCTCCCCGACTTGCAGG
  AAGTGAAGCGCGTTCTCTATGGCAAGGAACTCAGGAAGCTTGATCTGCCCAGGGAAGCTTTCGAAGCTGC
  CTCCAGAGAAGACTTTGAACTGCAGGGATATGCCTTTGAAGCAGCGGAGGAGCAGCTGAGACGACCCCGC
  ATTGTGCACGTGGGGCTGGTTCAGAACAGAATCCCCCTCCCCGCAAATGCCCCTGTGGCAGAACAGGTCT
  CTGCCCTTCATAGACGCATAAAGGCTATCGTAGAGGTGGCTGCAATGTGTGGAGTCAACATCATCTGTTT
  CCAGGAAGCATGGACTATGCCCTTTGCCTTCTGTACGAGAGAGAAGCTTCCTTGGACAGAATTTGCTGAG
  TCAGCAGAGGATGGGCCCACCACCAGATTCTGTCAGAAGCTGGCGAAGAACCATGACATGGTGGTGGTGT
  CTCCCATCCTGGAACGAGACAGCGAGCATGGGGATGTTTTGTGGAATACAGCCGTGGTGATCTCCAATTC
  CGGAGCAGTCCTGGGAAAGACCAGGAAAAACCACATCCCCAGAGTGGGTGATTTCAACGAGTCAACTTAC
  TACATGGAGGGAAACCTGGGCCACCCCGTGTTCCAGACGCAGTTCGGAAGGATCGCGGTGAACATTTGCT
  ACGGGCGGCACCACCCCCTCAACTGGCTTATGTACAGCATCAACGGGGCTGAGATCATCTTCAACCCCTC
  GGCCACGATAGGAGCACTCAGCGAGTCCCTGTGGCCCATCGAGGCCAGAAACGCAGCCATTGCCAATCAC
  TGCTTCACCTGCGCCATCAATCGAGTGGGCACCGAGCACTTCCCGAACGAGTTTACCTCGGGAGATGGAA
  AGAAAGCTCACCAGGACTTTGGCTACTTTTATGGCTCGAGCTATGTGGCAGCCCCTGACAGCAGCCGGAC
  TCCTGGGCTGTCCCGTAGCCGGGATGGACTGCTAGTTGCTAAGCTCGACCTAAACCTCTGCCAGCAGGTG
  AATGATGTCTGGAACTTCAAGATGACGGGCAGGTATGAGATGTACGCACGGGAGCTCGCCGAAGCTGTCA
  AGTCCAACTACAGCCCCACCATCGTGAAAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223365 representing NM_016327
  Red=Cloning site Green=Tags(s)
  
  MAGAEWKSLEECLEKHLPLPDLQEVKRVLYGKELRKLDLPREAFEAASREDFELQGYAFEAAEEQLRRPR
  IVHVGLVQNRIPLPANAPVAEQVSALHRRIKAIVEVAAMCGVNIICFQEAWTMPFAFCTREKLPWTEFAE
  SAEDGPTTRFCQKLAKNHDMVVVSPILERDSEHGDVLWNTAVVISNSGAVLGKTRKNHIPRVGDFNESTY
  YMEGNLGHPVFQTQFGRIAVNICYGRHHPLNWLMYSINGAEIIFNPSATIGALSESLWPIEARNAAIANH
  CFTCAINRVGTEHFPNEFTSGDGKKAHQDFGYFYGSSYVAAPDSSRTPGLSRSRDGLLVAKLDLNLCQQV
  NDVWNFKMTGRYEMYARELAEAVKSNYSPTIVKE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016327
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016327.3
• RefSeq Size: 2167 bp
• RefSeq ORF: 1155 bp
• Locus ID: 51733
• UniProt ID: Q9UBR1
• Cytogenetics: 22q11.23
• Domains: CN_hydrolase
• Protein Pathways: beta-Alanine metabolism, Drug metabolism - other enzymes, Metabolic pathways, Pantothenate and CoA biosynthesis, Pyrimidine metabolism
• Gene Summary: This gene encodes a protein that belongs to the CN hydrolase family. Beta-ureidopropionase catalyzes the last step in the pyrimidine degradation pathway. The pyrimidine bases uracil and thymine are degraded via the consecutive action of dihydropyrimidine dehydrogenase (DHPDH), dihydropyrimidinase (DHP) and beta-ureidopropionase (UP) to beta-alanine and beta-aminoisobutyric acid, respectively. UP deficiencies are associated with N-carbamyl-beta-amino aciduria and may lead to abnormalities in neurological activity. [provided by RefSeq, Jul 2008]