Claudin 10 (CLDN10) (NM_182848) Human Tagged ORF Clone

CAT#: RG221281

CLDN10 (tGFP-tagged) - Human claudin 10 (CLDN10), transcript variant a

Product Images

Circular map for RG221281

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Claudin 10
• Synonyms: CPETRL3; HELIX; OSP-L; OSPL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221281 representing NM_182848
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGGGCGCAGATCTGGGCTCTGGTGTCTGGTGTCGGAGGGTTTGGAGCTCTCGTTGCTGCTACCA
  CGTCCAATGAGTGGAAAGTGACCACGCGAGCCTCCTCGGTGATAACAGCCACTTGGGTTTACCAGGGTCT
  GTGGATGAACTGCGCAGGTAACGCGTTGGGTTCTTTCCATTGCCGACCGCATTTTACTATCTTCAAAGTA
  GCAGGTTATATACAGGCATGTAGAGGACTTATGATCGCTGCTGTCAGCCTGGGCTTCTTTGGTTCCATAT
  TTGCGCTCTTTGGAATGAAGTGTACCAAAGTCGGAGGCTCCGATAAAGCCAAAGCTAAAATTGCTTGTTT
  GGCTGGGATTGTATTCATACTGTCAGGGCTGTGCTCAATGACTGGATGTTCCCTATATGCAAACAAAATC
  ACAACGGAATTCTTTGATCCTCTCTTTGTTGAGCAAAAGTATGAATTAGGAGCCGCTCTGTTTATTGGAT
  GGGCAGGAGCCTCACTGTGCATAATTGGTGGTGTCATATTTTGCTTTTCAATATCTGACAACAACAAAAC
  ACCCAGATACACATACAACGGGGCCACATCTGTCATGTCTTCTCGGACAAAGTATCATGGTGGAGAAGAT
  TTTAAAACAACAAACCCTTCAAAACAGTTTGATAAAAATGCTTATGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221281 representing NM_182848
  Red=Cloning site Green=Tags(s)
  
  MSRAQIWALVSGVGGFGALVAATTSNEWKVTTRASSVITATWVYQGLWMNCAGNALGSFHCRPHFTIFKV
  AGYIQACRGLMIAAVSLGFFGSIFALFGMKCTKVGGSDKAKAKIACLAGIVFILSGLCSMTGCSLYANKI
  TTEFFDPLFVEQKYELGAALFIGWAGASLCIIGGVIFCFSISDNNKTPRYTYNGATSVMSSRTKYHGGED
  FKTTNPSKQFDKNAYV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182848
• ORF Size: 678 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182848.3, NP_878268.1
• RefSeq Size: 2658 bp
• RefSeq ORF: 681 bp
• Locus ID: 9071
• UniProt ID: P78369
• Cytogenetics: 13q32.1
• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction
• Gene Summary: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The expression level of this gene is associated with recurrence of primary hepatocellular carcinoma. Six alternatively spliced transcript variants encoding different isoforms have been reported, but the transcript sequences of some variants are not determined.[provided by RefSeq, Jun 2010]