PER3 (NM_016831) Human Tagged ORF Clone

CAT#: RG217739

PER3 (tGFP-tagged) - Human period homolog 3 (Drosophila) (PER3)

Product Images

Circular map for RG217739

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PER3
• Synonyms: FASPS3; GIG13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217739 representing NM_016831
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGCGGGGAAGCTCCTGGCCCCGGGAGACGGGGGGCTAAGGACGAGGCCCTGGGCGAAGAATCGG
  GGGAGCGGTGGAGCCCCGAGTTCCATCTGCAGAGGAAATTGGCGGACAGCAGCCACAGTGAACAGCAAGA
  TCGAAACAGAGTTTCTGAAGAACTTATCATGGTTGTCCAAGAAATGAAAAAATACTTCCCCTCGGAGAGA
  CGCAATAAACCAAGCACTCTAGATGCCCTCAACTATGCTCTCCGCTGTGTCCACAGCGTTCAAGCAAACA
  GTGAGTTTTTCCAGATTCTCAGTCAGAATGGAGCACCTCAGGCAGATGTGAGCATGTACAGTCTTGAGGA
  GCTGGCCACTATCGCTTCAGAACACACTTCCAAAAACACAGATACCTTTGTGGCAGTATTTTCATTTCTG
  TCTGGAAGGTTAGTGCACATTTCTGAACAGGCTGCTTTGATCCTGAATCGTAAGAAAGATGTCCTGGCGT
  CTTCTCACTTTGTTGACCTGCTTGCACCTCAAGACATGAGGGTATTCTACGCGCACACTGCCAGAGCTCA
  GCTTCCTTTCTGGAACAACTGGACCCAAAGAGCTGCACGGTATGAATGTGCTCCGGTGAAACCTTTTTTC
  TGCAGGATCCGTGGAGGTGAAGACAGAAAGCAAGAGAAGTGTCACTCCCCATTCCGGATCATCCCCTATC
  TGATTCATGTACATCACCCTGCCCAGCCAGAATTGGAATCGGAACCTTGCTGTCTCACTGTGGTTGAAAA
  GATTCACTCTGGTTATGAAGCTCCTCGGATCCCAGTGAATAAAAGAATCTTCACCACCACACACACCCCA
  GGGTGTGTTTTTCTTGAAGTAGATGAAAAAGCAGTGCCTTTGCTGGGTTACCTACCTCAGGACCTGATTG
  GAACATCGATCCTAAGCTACCTGCACCCTGAAGATCGTTCTCTGATGGTTGCCATACACCAAAAAGTTTT
  GAAGTATGCAGGGCATCCTCCCTTTGAACATTCTCCCATTCGATTTTGTACTCAAAACGGAGACTACATC
  ATACTGGATTCCAGTTGGTCCAGCTTTGTGAATCCCTGGAGCCGGAAGATTTCTTTCATCATTGGTCGGC
  ATAAAGTTCGAACGAGCCCACTAAATGAGGATGTTTTTGCTACCAAAATTAAAAAGATGAACGATAATGA
  CAAAGACATAACAGAATTACAAGAACAAATTTACAAACTTCTCTTACAGCCAGTTCACGTGAGCGTGTCC
  AGCGGCTACGGGAGCCTGGGGAGCAGCGGGTCGCAGGAGCAGCTTGTCAGCATCGCCTCCTCCAGTGAGG
  CCAGTGGGCACCGTGTGGAGGAGACGAAGGCGGAGCAGATGACCTTGCAGCAGGTCTATGCCAGTGTGAA
  CAAAATTAAAAATCTGGGTCAGCAGCTCTACATTGAGTCAATGACCAAATCATCATTCAAGCCAGTGACG
  GGGACACGCACAGAACCGAATGGTGGTGGTGAATGTAAGACCTTTACTTCCTTCCACCAAACACTGAAAA
  ACAATAGTGTGTACACTGAGCCCTGTGAGGATTTGAGGAACGATGAGCACAGCCCATCCTATCAACAGAT
  CAACTGTATCGACAGTGTCATCAGATACCTGAAGAGCTACAACATTCCAGCTTTGAAAAGAAAGTGTATC
  TCCTGTACAAATACAACTTCTTCCTCCTCAGAAGAAGACAAACAGAACCACAAGGCAGATGATGTCCAAG
  CCTTACAAGCTGGTTTGCAAATCCCAGCCATACCTAAATCAGAAATGCCAACAAATGGACGGTCCATAGA
  CACAGGAGGAGGAGCTCCACAGATCCTGTCCACGGCGATGCTGAGCTTGGGGTCGGGCATAAGCCAATGC
  GGTTACAGCAGCACCATTGTCCATGTCCCACCCCCAGAGACAGCCAGGGATGCTACCCTCTTCTGTGAGC
  CCTGGACCCTGAACATGCAGCCAGCCCCTTTGACCTCGGAAGAATTTAAACACGTGGGGCTCACAGCGGC
  TGTTCTGTCAGCGCACACCCAGAAGGAAGAGCAGAATTATGTTGATAAATTCCGAGAAAAGATCCTGTCA
  TCACCCTACAGCTCCTATCTTCAGCAAGAAAGCAGGAGCAAAGCTAAATATTCATATTTTCAAGGAGATT
  CTACTTCCAAGCAGACGCGGTCGGCCGGCTGCAGGAAAGGGAAGCACAAGCGGAAGAAGCTGCCGGAGCC
  GCCAGACAGCAGCAGCTCGAACACCGGCTCTGGTCCCCGCAGGGGAGCGCATCAGAACGCACAGCCCTGC
  TGCCCCTCCGCGGCCTCCTCTCCGCACACCTCGAGCCCGACCTTCCCACCTGCCGCCATGGTGCCCAGCC
  AGGCCCCTTACCTCGTCCCAGCTTTTCCCCTCCCAGCCGCGACCTCACCCGGAAGAGAATACGCAGCCCC
  CGGAACTGCACCGGAAGGCCTGCATGGGCTGCCCTTGTCCGAGGGCTTGCAGCCTTACCCAGCTTTCCCT
  TTTCCTTACTTGGATACTTTTATGACCGTTTTCCTGCCTGACCCCCCTGTCTGTCCTCTGTTGTCGCCAT
  CGTTTTTGCCATGTCCATTCCTGGGGGCGACAGCCTCTTCTGCGATATCACCCTCAATGTCGTCAGCAAT
  GAGTCCAACTCTGGACCCACCCCCTTCAGTCACCAGCCAAAGGAGAGAGGAGGAAAAGTGGGAGGCACAA
  AGCGAGGGGCACCCGTTCATTACTTCGAGAAGCAGCTCACCCTTGCAGTTAAACTTACTTCAGGAAGAGA
  TGCCCAGACCCTCTGAATCTCCAGATCAGATGAGAAGGAACACGTGCCCACAAACTGAGTATTGTGTTAC
  AGGCAACAATGGCAGTGAGAGCAGTCCTGCTACTACCGGTGCACTGTCCACGGGGTCACCTCCCAGGGAG
  AATCCATCCCATCCTACTGCCAGCGCTCTGTCCACAGGATCGCCTCCCATGAAGAATCCATCCCATCCTA
  CTGCCAGCGCTCTGTCCACAGGATCGCCTCCCATGAAGAATCCATCCCATCCTACTGCCAGCACACTGTC
  CATGGGATTGCCTCCCAGCAGGACTCCATCCCATCCTACTGCCACTGTTCTGTCCACGGGGTCACCTCCC
  AGCGAATCCCCATCCAGAACTGGTTCAGCAGCATCAGGAAGCAGCGACAGCAGTATATACCTTACTAGTA
  GTGTTTATTCTTCTAAAATCTCCCAAAATGGGCAGCAATCTCAGGACGTACAGAAAAAAGAAACATTTCC
  TAATGTCGCCGAAGAGCCCATCTGGAGAATGATACGGCAGACACCTGAGCGCATTCTCATGACATACCAG
  GTACCTGAGAGGGTTAAAGAAGTTGTACTAAAAGAAGACCTGGAAAAGCTAGAAAGTATGAGGCAGCAGC
  AGCCCCAGTTTTCTCATGGGCAAAAGGAGGAGCTGGCTAAGGTGTATAATTGGATTCAAAGCCAGACTGT
  CACTCAAGAAATCGACATTCAAGCCTGTGTCACTTGTGAAAATGAAGATTCAGCTGATGGTGCGGCCACA
  TCCTGTGGTCAGGTTCTGGTAGAAGACAGCTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217739 representing NM_016831
  Red=Cloning site Green=Tags(s)
  
  MPRGEAPGPGRRGAKDEALGEESGERWSPEFHLQRKLADSSHSEQQDRNRVSEELIMVVQEMKKYFPSER
  RNKPSTLDALNYALRCVHSVQANSEFFQILSQNGAPQADVSMYSLEELATIASEHTSKNTDTFVAVFSFL
  SGRLVHISEQAALILNRKKDVLASSHFVDLLAPQDMRVFYAHTARAQLPFWNNWTQRAARYECAPVKPFF
  CRIRGGEDRKQEKCHSPFRIIPYLIHVHHPAQPELESEPCCLTVVEKIHSGYEAPRIPVNKRIFTTTHTP
  GCVFLEVDEKAVPLLGYLPQDLIGTSILSYLHPEDRSLMVAIHQKVLKYAGHPPFEHSPIRFCTQNGDYI
  ILDSSWSSFVNPWSRKISFIIGRHKVRTSPLNEDVFATKIKKMNDNDKDITELQEQIYKLLLQPVHVSVS
  SGYGSLGSSGSQEQLVSIASSSEASGHRVEETKAEQMTLQQVYASVNKIKNLGQQLYIESMTKSSFKPVT
  GTRTEPNGGGECKTFTSFHQTLKNNSVYTEPCEDLRNDEHSPSYQQINCIDSVIRYLKSYNIPALKRKCI
  SCTNTTSSSSEEDKQNHKADDVQALQAGLQIPAIPKSEMPTNGRSIDTGGGAPQILSTAMLSLGSGISQC
  GYSSTIVHVPPPETARDATLFCEPWTLNMQPAPLTSEEFKHVGLTAAVLSAHTQKEEQNYVDKFREKILS
  SPYSSYLQQESRSKAKYSYFQGDSTSKQTRSAGCRKGKHKRKKLPEPPDSSSSNTGSGPRRGAHQNAQPC
  CPSAASSPHTSSPTFPPAAMVPSQAPYLVPAFPLPAATSPGREYAAPGTAPEGLHGLPLSEGLQPYPAFP
  FPYLDTFMTVFLPDPPVCPLLSPSFLPCPFLGATASSAISPSMSSAMSPTLDPPPSVTSQRREEEKWEAQ
  SEGHPFITSRSSSPLQLNLLQEEMPRPSESPDQMRRNTCPQTEYCVTGNNGSESSPATTGALSTGSPPRE
  NPSHPTASALSTGSPPMKNPSHPTASALSTGSPPMKNPSHPTASTLSMGLPPSRTPSHPTATVLSTGSPP
  SESPSRTGSAASGSSDSSIYLTSSVYSSKISQNGQQSQDVQKKETFPNVAEEPIWRMIRQTPERILMTYQ
  VPERVKEVVLKEDLEKLESMRQQQPQFSHGQKEELAKVYNWIQSQTVTQEIDIQACVTCENEDSADGAAT
  SCGQVLVEDSC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016831
• ORF Size: 3603 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016831.4
• RefSeq Size: 6203 bp
• RefSeq ORF: 3606 bp
• Locus ID: 8863
• UniProt ID: P56645
• Cytogenetics: 1p36.23
• Domains: PAS
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]