SPHKAP (NM_030623) Human Tagged ORF Clone

CAT#: RG215958

SPHKAP (tGFP-tagged) - Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 2

Product Images

Circular map for RG215958

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SPHKAP
• Synonyms: SKIP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215958 representing NM_030623
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGCAACTCCCTGCTCTCGGTACCAAGCAACTTGGAGTCATCACGGATGTATGACGTTTTGGAAC
  CGCAGCAGGGCAGAGGCTGTGGCAGCTCAGGAAGCGGCCCGGGGAACTCCATCACAGCCTGTAAGAAGGT
  TCTTCGCAGCAATAGCCTGCTGGAGTCAACAGACTACTGGTTGCAGAATCAGAGGATGCCCTGCCAAATT
  GGTTTTGTAGAAGACAAGTCTGAAAACTGTGCTTCTGTCTGCTTTGTGAATCTTGGTGTGAACAAGGATG
  AATGCAGCACAGAGCACCTGCAACAGAAACTGGTCAACGTTTCACCAGATCTTCCAAAACTTATCAGTTC
  CATGAATGTCCAACAACCAAAAGAAAATGAAATTGTTGTCCTAAGTGGGTTAGCCTCTGGAAATCTCCAG
  GCAGATTTTGAAGTTTCACAGTGCCCTTGGCTGCCAGATATCTGCTTGGTCCAATGTGCAAGAGGGAACA
  GACCAAACAGTACCAACTGCATCATCTTTGAAATCAACAAATTTCTGATTGGTCTGGAACTGGTGCAGGA
  GCGACAGCTCCACCTGGAAACAAACATCTTGAAACTGGAGGATGACACGAACTGTTCCTTATCTTCAATC
  GAGGAAGACTTTCTCACCGCTTCTGAGCACTTGGAGGAGGAAAGCGAGGTGGATGAATCTAGGAACGATT
  ATGAAAATATAAATGTCTCAGCCAATGTTTTGGAAAGTAAACAGCTAAAGGGAGCCACCCAGGTGGAATG
  GAATTGCAACAAGGAAAAGTGGCTTTATGCTTTGGAAGACAAATACATCAACAAATATCCCACACCATTG
  ATTAAAACAGAACGATCTCCAGAAAACCTAACAAAGAACACAGCCTTGCAGAGTCTAGATCCCTCAGCCA
  AGCCATCACAGTGGAAAAGAGAAGCTGTGGGGAATGGGAGACAAGCCACACATTATTATCATTCAGAAGC
  TTTTAAAGGTCAAATGGAAAAATCACAGGCACTGTATATTCCAAAAGATGCTTATTTCTCCATGATGGAT
  AAAGATGTACCTTCTGCATGTGCTGTGGCAGAGCAGAGAAGCAACCTAAACCCAGGAGACCATGAAGACA
  CAAGAAACGCTCTCCCTCCTAGACAAGATGGAGAAGTCACCACTGGCAAGTATGCTACAAATTTAGCAGA
  ATCCGTGCTGCAGGATGCATTTATTAGATTATCTCAATCTCAGTCCACATTACCCCAGGAATCTGCAGTC
  AGTGTTTCTGTAGGAAGTTCTCTGCTTCCCAGTTGCTATTCCACAAAAGATACAGTGGTTTCTCGGTCAT
  GGAATGAGCTCCCCAAAATCGTCGTTGTTCAGAGTCCAGATGGCAGTGATGCTGCCCCACAGCCAGGCAT
  CTCCTCCTGGCCTGAGATGGAAGTCTCTGTTGAAACCTCAAGCATCCCCTCTGGAGAGAACTCCAGCAGA
  CAACCCCAGAGTGCTCTAGAAGTGGCGTTAGCTTGTGCAGCCACTGTGATTGGAACTATTTCCAGTCCAC
  AGGCCACAGAAAGACTCAAAATGGAGCAAGTGGTCTCGAACTTTCCCCCAGGGAGCAGTGGTGCACTGCA
  AACTCAAGCACCCCAAGGACTCAAGGAACCTTCCATCAATGAGTACTCCTTTCCATCTGCTTTGTGTGGC
  ATGACTCAGGTGGCCAGTGCCGTGGCTGTCTGTGGTCTGGGTGAAAGAGAAGAGGTGACATGCTCAGTGG
  CTCCAAGTGGTAGCCTCCCGCCTGCAGCTGAGGCTTCTGAAGCCATGCCCCCACTTTGTGGTTTAGCAAG
  CATGGAGCTTGGCAAGGAAGCCATTGCCAAGGGATTGCTCAAGGAGGCTGCTCTGGTTTTAACAAGGCCT
  AATACCTACAGCAGCATTGGAGACTTTCTGGACTCCATGAACAGGAGAATCATGGAAACTGCTTCAAAGT
  CTCAGACCCTGTGCTCAGAAAATGTCGTCAGGAATGAACTGGCACATACCCTGTCCAATGTTATCCTGAG
  GCATTCCATTGATGAAGTTCACCACAAAAATATGATAATCGACCCCAATGACAACAGGCATTCATCTGAA
  ATTCTGGACACCTTAATGGAAAGTACAAATCAACTGCTTTTAGATGTGATATGCTTCACGTTCAAGAAGA
  TGAGTCATATTGTACGGCTTGGTGAATGTCCTGCTGTCCTTTCTAAGGAGACCATCAGAAGGAGGGAGAC
  AGAACCAAGCTGCCAGCCATCTGATCCGGGTGCTAGTCAAGCTTGGACAAAAGCCACTGAATCCTCCAGC
  AGCTCTCCACTTAGCAATTCACACAACACGAGTCTTGTCATCAACAATCTTGTGGATGGCATGTATTCAA
  AACAAGACAAGGGTGGAGTGAGGCCAGGCCTCTTCAAGAACCCCACGCTGCAGTCACAATTATCACGTAG
  TCACAGAGTGCCCGATTCTTCAACTGCTACAACATCCTCCAAGGAAATATATCTGAAAGGAATAGCAGGA
  GAGGATACAAAAAGCCCTCATCACAGTGAGAATGAATGCAGAGCCTCTTCCGAAGGACAAAGGTCCCCAA
  CGGTCAGCCAGTCCAGAAGTGGTTCCCAGGAGGCTGAGGAGAGTATCCACCCAAACACCCAAGAAAAGTA
  CAACTGTGCCACATCTCGCATCAACGAAGTTCAAGTCAACCTGTCCTTGTTAGGGGATGACCTGCTGCTT
  CCTGCTCAATCCACGCTTCAAACAAAGCATCCAGACATCTACTGCATTACAGACTTTGCGGAAGAATTAG
  CAGACACGGTCGTCTCCATGGCAACTGAAATTGCAGCGATTTGCCTTGACAACTCCAGTGGAAAACAACC
  CTGGTTTTGTGCATGGAAAAGAGGGAGTGAGTTTCTGATGACACCCAACGTACCCTGCCGATCCTTGAAG
  AGGAAGAAAGAGAGCCAGGGGAGCGGGACCGCTGTGAGGAAACACAAGCCTCCCCGGCTCAGTGAGATCA
  AGAGGAAGACGGACGAGCACCCTGAGCTTAAAGAAAAGCTGATGAACAGGGTTGTGGATGAGTCCATGAA
  CCTTGAAGATGTCCCAGATTCTGTCAATCTTTTTGCCAATGAAGTGGCAGCCAAGATCATGAACCTAACG
  GAGTTCTCTATGGTGGACGGCATGTGGCAGGCGCAGGGCTATCCCCGGAATCGGTTACTGAGTGGCGACA
  GGTGGAGCCGGCTGAAGGCCTCCAGCTGCGAAAGCATTCCTGAGGAAGACTCCGAGGCCAGGGCCTATGT
  CAACAGCCTGGGCTTAATGAGCACGCTGAGCCAGCCGGTCAGCAGGGCCAGCTCTGTCTCCAAGCAGTCG
  AGCTGTGAGAGCATCACCGATGAGTTTTCCAGGTTCATGGTGAACCAGATGGAAAATGAAGGGAGAGGAT
  TTGAGTTACTGCTGGATTACTATGCTGGCAAGAACGCCAGCAGCATTCTGAACTCAGCCATGCAACAGGC
  GTGCCGGAAAAGTGACCACCTCAGTGTGAGGCCTAGCTGTCCCTCTAAGCAGTCCAGCACAGAGAGCATC
  ACTGAGGAGTTCTACAGGTACATGCTGAGGGACATCGAAAGAGACAGCAGAGAAAGTGCCTCCTCCAGAC
  GGAGCAGCCAGATTTGGACAGCCGGCCTGCTGTCTCCTTCTCTGCGATCCCCAGTGTGCCACAGACAGTC
  GTCCATGCCAGACAGCAGATCCCCATGCTCCAGGCTGACAGTGAATGTGCCCATCAAAGCCAACTCTTTA
  GATGGCTTTGCTCAGAACTGCCCACAAGATTTCCTAAGCGTGCAGCCGGTCAGTAGCGCGTCCTCATCCG
  GTCTCTGCAAATCTGACTCTTGCTTGTATCGGAGAGGTGGGACTGACCACATCACCAACATGTTAATTCA
  TGAAACGTGGGCTAGCTCCATTGAGGCTCTCATGCGCAAGAACAAAATCATTGTGGATGATGCAGAGGAA
  GCTGACACTGAGCCTGTTTCTGGTGGCTCTCCCTCGCAAGCAGAGAAGTGTGCAAATAGATTAGCTGCGA
  GCAGGATGTGCAGTGGGCCAACTCTGCTTGTTCAGGAGTCTCTCGATTGCCCGAGGAAAGACTCTGTTAC
  CGAATGTAAACAGCCCCCAGTGTCATCTTTGAGCAAAACTGCTTCTCTTACAAACCACAGCCCTTTAGAT
  TCTAAAAAAGAAACTTCCTCGTGCCAGGACCCTGTACCAATAAACCACAAAAGGCGATCACTTTGCTCGA
  GGGAAGTGCCTTTGATTCAGATTGAAACAGATCAGAGAGAAGCCTGTGCTGGGGAACCTGAACCCTTCCT
  TTCCAAAAGCAGCCTCCTAGAGGAAGCAGAAGGGCATTCGAATGACAAAAACATCCCAGATGTGGTGAGA
  GGTGGAGACACAGCCGTGAGCGCTTGTCAAATCCATAGTGACAGCCTTGATACCAGAGATGTACCAGAGG
  CTGAAGCCTCCACAGAAGCCAGAGCCCCCGATGAGGCCCCCAACCCTCCAAGCAGCAGCGAGGAGAGCAC
  AGGCAGCTGGACCCAGCTTGCCAATGAGGAAGACAACCCAGATGACACAAGTAGCTTTCTCCAGCTCAGT
  GAGCGATCCATGAGTGAATTAGTAGAAGAAAAGAAGATTCTTAAAGGACAGTCAGAAAGCACAGAGGCAC
  CTGCATCTGGACCGCCTACGGGAACAGCCAGCCCCCAGAGGAGCCTGCTGGTGATCAACTTTGACCTGGA
  GCCAGAGTGTCCAGATGCCGAGCTCCGAGCCACTCTGCAGTGGATAGCTGCCTCTGAACTGGGGATTCCC
  ACCATCTACTTTAAGAAATCTCAGGAAAACAGAATTGAAAAGTTTCTAGATGTCGTGCAGCTGGTTCATC
  GGAAGTCCTGGAAAGTGGGTGATATCTTCCATGCAGTTGTCCAGTACTGCAAAATGCATGAGGAGCAGAA
  GGATGGGAGACTGAGTCTCTTTGACTGGCTCTTGGAACTGGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215958 representing NM_030623
  Red=Cloning site Green=Tags(s)
  
  MDGNSLLSVPSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQI
  GFVEDKSENCASVCFVNLGVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQ
  ADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSI
  EEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPL
  IKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMD
  KDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAV
  SVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSIPSGENSSR
  QPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCG
  MTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRP
  NTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSE
  ILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSS
  SSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAG
  EDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLL
  PAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLK
  RKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLT
  EFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQS
  SCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESI
  TEEFYRYMLRDIERDSRESASSRRSSQIWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSL
  DGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEE
  ADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLD
  SKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVR
  GGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLS
  ERSMSELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIP
  TIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCKMHEEQKDGRLSLFDWLLELG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030623
• ORF Size: 5013 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030623.2
• RefSeq Size: 6825 bp
• RefSeq ORF: 5016 bp
• Locus ID: 80309
• UniProt ID: Q2M3C7
• Cytogenetics: 2q36.3
• Protein Families: Druggable Genome
• Gene Summary: Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.[UniProtKB/Swiss-Prot Function]