ABCB4 (NM_018850) Human Tagged ORF Clone

CAT#: RG212987

ABCB4 (tGFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 (ABCB4), transcript variant C

Product Images

Circular map for RG212987

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ABCB4
• Synonyms: ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3; PFIC-3; PGY3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212987 representing NM_018850
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTTGAGGCGGCAAAGAACGGAACAGCCTGGCGCCCCACGAGCGCGGAGGGCGACTTTGAACTGG
  GCATCAGCAGCAAACAAAAAAGGAAAAAAACGAAGACAGTGAAAATGATTGGAGTATTAACATTGTTTCG
  ATACTCCGATTGGCAGGATAAATTGTTTATGTCGCTGGGTACCATCATGGCCATAGCTCACGGATCAGGT
  CTCCCCCTCATGATGATAGTATTTGGAGAGATGACTGACAAATTTGTTGATACTGCAGGAAACTTCTCCT
  TTCCAGTGAACTTTTCCTTGTCGCTGCTAAATCCAGGCAAAATTCTGGAAGAAGAAATGACTAGATATGC
  ATATTACTACTCAGGATTGGGTGCTGGAGTTCTTGTTGCTGCCTATATACAAGTTTCATTTTGGACTTTG
  GCAGCTGGTCGACAGATCAGGAAAATTAGGCAGAAGTTTTTTCATGCTATTCTACGACAGGAAATAGGAT
  GGTTTGACATCAACGACACCACTGAACTCAATACGCGGCTAACAGATGACATCTCCAAAATCAGTGAAGG
  AATTGGTGACAAGGTTGGAATGTTCTTTCAAGCAGTAGCCACGTTTTTTGCAGGATTCATAGTGGGATTC
  ATCAGAGGATGGAAGCTCACCCTTGTGATAATGGCCATCAGCCCTATTCTAGGACTCTCTGCAGCCGTTT
  GGGCAAAGATACTCTCGGCATTTAGTGACAAAGAACTAGCTGCTTATGCAAAAGCAGGCGCCGTGGCAGA
  AGAGGCTCTGGGGGCCATCAGGACTGTGATAGCTTTCGGGGGCCAGAACAAAGAGCTGGAAAGGTATCAG
  AAACATTTAGAAAATGCCAAAGAGATTGGAATTAAAAAAGCTATTTCAGCAAACATTTCCATGGGTATTG
  CCTTCCTGTTAATATATGCATCATATGCACTGGCCTTCTGGTATGGATCCACTCTAGTCATATCAAAAGA
  ATATACTATTGGAAATGCAATGACAGTTTTTTTTTCAATCCTAATTGGAGCTTTCAGTGTTGGCCAGGCT
  GCCCCATGTATTGATGCTTTTGCCAATGCAAGAGGAGCAGCATATGTGATCTTTGATATTATTGATAATA
  ATCCTAAAATTGACAGTTTTTCAGAGAGAGGACACAAACCAGACAGCATCAAAGGGAATTTGGAGTTCAA
  TGATGTTCACTTTTCTTACCCTTCTCGAGCTAACGTCAAGATCTTGAAGGGCCTCAACCTGAAGGTGCAG
  AGTGGGCAGACGGTGGCCCTGGTTGGAAGTAGTGGCTGTGGGAAGAGCACAACGGTCCAGCTGATACAGA
  GGCTCTATGACCCTGATGAGGGCACAATTAACATTGATGGGCAGGATATTAGGAACTTTAATGTAAACTA
  TCTGAGGGAAATCATTGGTGTGGTGAGTCAGGAGCCGGTGCTGTTTTCCACCACAATTGCTGAAAATATT
  TGTTATGGCCGTGGAAATGTAACCATGGATGAGATAAAGAAAGCTGTCAAAGAGGCCAACGCCTATGAGT
  TTATCATGAAATTACCACAGAAATTTGACACCCTGGTTGGAGAGAGAGGGGCCCAGCTGAGTGGTGGGCA
  GAAGCAGAGGATCGCCATTGCACGTGCCCTGGTTCGCAACCCCAAGATCCTTCTGCTGGATGAGGCCACG
  TCAGCATTGGACACAGAAAGTGAAGCTGAGGTACAGGCAGCTCTGGATAAGGCCAGAGAAGGCCGGACCA
  CCATTGTGATAGCACACCGACTGTCTACGGTCCGAAATGCAGATGTCATCGCTGGGTTTGAGGATGGAGT
  AATTGTGGAGCAAGGAAGCCACAGCGAACTGATGAAGAAGGAAGGGGTGTACTTCAAACTTGTCAACATG
  CAGACATCAGGAAGCCAGATCCAGTCAGAAGAATTTGAACTAAATGATGAAAAGGCTGCCACTAGAATGG
  CCCCAAATGGCTGGAAATCTCGCCTATTTAGGCATTCTACTCAGAAAAACCTTAAAAATTCACAAATGTG
  TCAGAAGAGCCTTGATGTGGAAACCGATGGACTTGAAGCAAATGTGCCACCAGTGTCCTTTCTGAAGGTC
  CTGAAACTGAATAAAACAGAATGGCCCTACTTTGTCGTGGGAACAGTATGTGCCATTGCCAATGGGGGGC
  TTCAGCCGGCATTTTCAGTCATATTCTCAGAGATCATAGCGATTTTTGGACCAGGCGATGATGCAGTGAA
  GCAGCAGAAGTGCAACATATTCTCTTTGATTTTCTTATTTCTGGGAATTATTTCTTTTTTTACTTTCTTC
  CTTCAGGGTTTCACGTTTGGGAAAGCTGGCGAGATCCTCACCAGAAGACTGCGGTCAATGGCTTTTAAAG
  CAATGCTAAGACAGGACATGAGCTGGTTTGATGACCATAAAAACAGTACTGGTGCACTTTCTACAAGACT
  TGCCACAGATGCTGCCCAAGTCCAAGGAGCCACAGGAACCAGGTTGGCTTTAATTGCACAGAATATAGCT
  AACCTTGGAACTGGTATTATCATATCATTTATCTACGGTTGGCAGTTAACCCTATTGCTATTAGCAGTTG
  TTCCAATTATTGCTGTGTCAGGAATTGTTGAAATGAAATTGTTGGCTGGAAATGCCAAAAGAGATAAAAA
  AGAACTGGAAGCTGCTGGAAAGATTGCAACAGAGGCAATAGAAAATATTAGGACAGTTGTGTCTTTGACC
  CAGGAAAGAAAATTTGAATCAATGTATGTTGAAAAATTGTATGGACCTTACAGGGTGTTTTCTGCAATTG
  TATTTGGTGCAGTGGCTCTAGGACATGCCAGTTCATTTGCTCCAGACTATGCTAAAGCTAAGCTGTCTGC
  AGCCCACTTATTCATGCTGTTTGAAAGACAACCTCTGATTGACAGCTACAGTGAAGAGGGGCTGAAGCCT
  GATAAATTTGAAGGAAATATAACATTTAATGAAGTCGTGTTCAACTATCCCACCCGAGCAAACGTGCCAG
  TGCTTCAGGGGCTGAGCCTGGAGGTGAAGAAAGGCCAGACACTAGCCCTGGTGGGCAGCAGTGGCTGTGG
  GAAGAGCACGGTGGTCCAGCTCCTGGAGCGGTTCTACGACCCCTTGGCGGGGACAGTGCTTCTCGATGGT
  CAAGAAGCAAAGAAACTCAATGTCCAGTGGCTCAGAGCTCAACTCGGAATCGTGTCTCAGGAGCCTATCC
  TATTTGACTGCAGCATTGCCGAGAATATTGCCTATGGAGACAACAGCCGGGTTGTATCACAGGATGAAAT
  TGTGAGTGCAGCCAAAGCTGCCAACATACATCCTTTCATCGAGACGTTACCCCACAAATATGAAACAAGA
  GTGGGAGATAAGGGGACTCAGCTCTCAGGAGGTCAAAAACAGAGGATTGCTATTGCCCGAGCCCTCATCA
  GACAACCTCAAATCCTCCTGTTGGATGAAGCTACATCAGCTCTGGATACTGAAAGTGAAAAGGTTGTCCA
  AGAAGCCCTGGACAAAGCCAGAGAAGGCCGCACCTGCATTGTGATTGCTCACCGCCTGTCCACCATCCAG
  AATGCAGACTTAATAGTGGTGTTTCAGAATGGGAGAGTCAAGGAGCATGGCACGCATCAGCAGCTGCTGG
  CACAGAAAGGCATCTATTTTTCAATGGTCAGTGTCCAGGCTGGGACACAGAACTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212987 representing NM_018850
  Red=Cloning site Green=Tags(s)
  
  MDLEAAKNGTAWRPTSAEGDFELGISSKQKRKKTKTVKMIGVLTLFRYSDWQDKLFMSLGTIMAIAHGSG
  LPLMMIVFGEMTDKFVDTAGNFSFPVNFSLSLLNPGKILEEEMTRYAYYYSGLGAGVLVAAYIQVSFWTL
  AAGRQIRKIRQKFFHAILRQEIGWFDINDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGF
  IRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQ
  KHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQA
  APCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFNDVHFSYPSRANVKILKGLNLKVQ
  SGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENI
  CYGRGNVTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEAT
  SALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELMKKEGVYFKLVNM
  QTSGSQIQSEEFELNDEKAATRMAPNGWKSRLFRHSTQKNLKNSQMCQKSLDVETDGLEANVPPVSFLKV
  LKLNKTEWPYFVVGTVCAIANGGLQPAFSVIFSEIIAIFGPGDDAVKQQKCNIFSLIFLFLGIISFFTFF
  LQGFTFGKAGEILTRRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIA
  NLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLT
  QERKFESMYVEKLYGPYRVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEGLKP
  DKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVLLDG
  QEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETR
  VGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQ
  NADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTQNL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018850
• ORF Size: 3696 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018850.2, NP_061338.1
• RefSeq Size: 3826 bp
• RefSeq ORF: 3699 bp
• Locus ID: 5244
• UniProt ID: P21439
• Cytogenetics: 7q21.12
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq, Jul 2008]